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(-)-carvone + NADH + H+
? + NAD+
1% activity compared to cyclohexanone
-
-
?
(1S,3S)-3-methylcyclohexanol + NAD+
(rac)-3-methylcyclohexanone + NADH + H+
125% activity compared to cyclohexanol
163% activity compared to cyclohexanone
-
r
(2E)-2-methylpent-2-enal + NADPH + H+
(2E)-2-methylpent-2-en-1-ol + NADP+
(2E)-3,7-dimethylocta-2,6-dienal + NADPH + H+
(2E)-3,7-dimethylocta-2,6-dien-1-ol + NADP+
(2E)-but-2-en-1-ol + NADP+
(2E)-but-2-enal + NADPH + H+
-
-
-
r
(2E)-but-2-enal + NADPH + H+
(2E)-but-2-en-1-ol + NADP+
yield 96% after 12 h
-
-
r
(2E)-dec-2-enal + NADPH + H+
(2E)-dec-2-en-1-ol + NADP+
16.7% of the activity with (2E)-but-2-enal, yield 91% after 12 h
-
-
?
(2E)-hex-2-enal + NADPH + H+
(2E)-hex-2-en-1-ol + NADP+
41.7% of the activity with (2E)-but-2-enal, yield 98% after 12 h
-
-
?
(2E)-oct-2-enal + NADPH + H+
(2E)-oct-2-en-1-ol + NADP+
30.2% of the activity with (2E)-but-2-enal, yield 69% after 12 h
plus 27% oct-2-enyl ester
-
?
(6S)-5,6-dihydro-6-methyl-4H-thieno[2,3b]thiopyran-4-one-7,7-dioxide + NADH + H+
(4S,6S)-5,6-dihydro-4-hydroxy-6-methyl-4H-thieno[2,3b]thiopyran-7,7-dioxide + NAD+
-
-
-
-
-
(E)-hex-2-en-1-ol + NAD+
(E)-hex-2-en-1-one + NADH
(R)-1-indanol + NAD+
1-indanone + NADH + H+
-
-
-
r
(R)-1-indanol + NAD+
?
62% of activity compared to (S)-1-indanol
-
-
?
(R)-2-butanol + NAD+
2-butanone + NADH + H+
(R)-2-heptanol + NAD+
2-heptanone + NADH + H+
(R)-2-hexanol + NAD+
2-hexanone + NADH + H+
(R)-2-octanol + NAD+
2-octanone + NADH + H+
(R)-2-pentanol + NAD+
2-pentanone + NADH + H+
(R)-2-phenylpropanol + NAD+
(R)-2-phenylpropanal + NADH + H+
(R)-3-methylcyclohexanone + NADH + H+
? + NAD+
2% activity compared to cyclohexanone
-
-
?
(R)-alpha-tetralol + NAD+
?
58% of activity compared to (S)-1-indanol
-
-
?
(R)-alpha-tetralol + NAD+
alpha-tetralone + NADH + H+
-
-
-
r
(R,S)-2-methylbutan-1-ol + NAD+
(R,S)-2-methyl-butan-1-one + NADH + H+
(S)-(+)-1-indanol + NAD+
indanone + NADH + H+
-
-
-
-
?
(S)-1-indanol + NAD+
1-indanone + NADH + H+
(S)-1-indanol + NAD+
?
-
-
-
?
(S)-1-phenyl-2-propanol + NAD+
phenylacetone + NADH + H+
-
9% activity compared to cyclohexanone
-
r
(S)-1-phenylethanol + NAD+
1-phenylethanone + NADH + H+
-
-
-
-
r
(S)-2-butanol + NAD+
2-butanone + NADH
-
-
-
-
r
(S)-2-butanol + NAD+
2-butanone + NADH + H+
(S)-2-heptanol + NAD+
2-heptanone + NADH + H+
-
331% of the activity with 2-propanol
-
-
r
(S)-2-hexanol + NAD+
2-hexanone + NADH + H+
-
38% of the activity with 2-propanol
-
-
r
(S)-2-methylbutan-1-ol + NAD+
(S)-2-methyl-butanal + NADH + H+
(S)-2-octanol + NAD+
2-octanone + NADH + H+
(S)-2-pentanol + NAD+
2-pentanone + NADH + H+
(S)-2-pentanol + NAD+
?
11% of activity compared to (S)-1-indanol
-
-
?
(S)-2-phenylpropanol + NAD+
(S)-2-phenylpropanal + NADH + H+
-
156% of the activity with 2-phenylethanol
-
-
?
(S)-4-phenylbutan-2-ol + NAD+
benzylacetone + NADH + H+
-
3% activity compared to cyclohexanone
-
r
(S)-alpha-tetralol + NAD+
?
12% of activity compared to (S)-1-indanol
-
-
?
(S)-alpha-tetralol + NAD+
alpha-tetralone + NADH + H+
(S)-heptan-2-ol + NAD+
2-heptanone + NADH + H+
-
-
-
r
(S)-pentan-2-ol + NAD+
2-pentanone + NADH + H+
-
-
-
r
(S)-perillylalcohol + NAD+
(S)-perillaldehyde + NADH + H+
52% activity compared to cyclohexanol
-
-
?
(Z)-hex-2-en-1-ol + NAD+
(Z)-hex-2-en-1-one + NADH
-
9.7% of the activity with ethanol
-
-
?
1,1-dichloroacetone + NADH + H+
1,1-dichloropropan-2-ol + NADH
-
1078% of the activity with phenyl trifluoromethyl ketone
-
-
?
1,2-hexanediol + NAD+
?
-
20% of the activity with (S)-N-benzyl-3-pyrrolidinol
-
-
?
1,2-pentanediol + NAD+
?
-
12% of the activity with (S)-N-benzyl-3-pyrrolidinol
-
-
?
1,2-propanediol + NAD+
hydroxyacetone + NADH
-
-
-
-
?
1,2-propanediol + NAD+
hydroxyacetone + NADH + H+
-
-
-
-
?
1,3-butanediol + NAD+
?
-
39% of the activity with (S)-N-benzyl-3-pyrrolidinol
-
-
?
1,3-propanediol + 2 NAD+ + H2O
? + 2 NADH + 2 H+
1,3-propanediol + NAD+
? + NADH
-
7% of the activity with ethanol
-
-
?
1-(3-bromophenyl)ethanol + NAD+
1-(3-bromophenyl)ethanone + NADH + H+
315% of the activity with 1-phenylethanol
-
-
?
1-(3-chlorophenyl)ethanol + NAD+
1-(3-chlorophenyl)ethanone + NADH + H+
205% of the activity with 1-phenylethanol
-
-
?
1-(4'-chlorophenyl)ethanol + NAD+
1-(4'-chlorophenyl)ethanone + NADH + H+
-
26% of the activity with (S)-(-)-1-phenylethanol
-
-
?
1-(4'-fluorophenyl)ethanol + NAD+
1-(4'-fluorophenyl)ethanone + NADH + H+
-
45% of the activity with (S)-1-phenylethanol
-
-
r
1-(4-bromophenyl)ethanol + NAD+
1-(4-bromophenyl)ethanone + NADH + H+
167% of the activity with 1-phenylethanol
-
-
?
1-(4-chlorophenyl)ethanol + NAD+
1-(4-chlorophenyl)ethanone + NADH + H+
151% of the activity with 1-phenylethanol
-
-
?
1-(4-fluorophenyl)ethanol + NAD+
1-(4-fluorophenyl)ethanone + NADH + H+
1-(4-methylphenyl)ethanol + NAD+
1-(4-methylphenyl)ethanone + NADH + H+
189% of the activity with 1-phenylethanol
-
-
?
1-(p-tolyl)-ethanol + NAD+
1-(4-methylphenyl)ethanone + NADH + H+
19% activity compared to cyclohexanol
26% activity compared to cyclohexanone
-
?
1-butanal + NADH + H+
1-butanol + NAD+
-
-
-
-
r
1-butanol + 2 NAD+ + H2O
butanoic acid + 2 NADH + 2 H+
1-butanol + NAD+
butanal + NADH
1-butanol + NAD+
butanal + NADH + H+
1-chloro-5-acetylfuro[2,3-c]pyridine + NADH + H+
1-chloro-5-(1-hydroxyethyl)furo[2,3-c]pyridine + NAD+
-
-
-
-
r
1-decalone + NADH + H+
decahydronaphthalen-1-ol + NAD+
85% of the activity compared to 1-phenyl-1,2-propanedione
-
-
?
1-decanol + NAD+
decanal + NADH + H+
1-dodecanol + NAD+
dodecanal + NADH
1-heptanol + NAD+
1-heptanal + NADH + H+
-
-
-
-
r
1-heptanol + NAD+
heptanal + NADH + H+
1-heptanol + NAD+
heptanol + NADH + H+
-
56% of the activity with 2-propanol, in the reverse reaction 755% of the activity with phenyl trifluoromethyl ketone
-
-
r
1-hexanal + NADH + H+
1-hexanol + NAD+
-
-
-
-
r
1-hexanol + 2 NAD+ + H2O
hexanoic acid + 2 NADH + 2 H+
1-hexanol + NAD+
1-hexanal + NADH + H+
1-hexanol + NAD+
?
-
15% of the activity with (S)-N-benzyl-3-pyrrolidinol
-
-
?
1-hexanol + NAD+
hexanal + NADH + H+
1-hydroxy-2-butanone + NADH + H+
butane-1,2-diol + NAD+
-
59% of the activity with N-benzyl-3-pyrrolidinone
-
-
?
1-hydroxymethyl-6-methylpyrene + NAD+
1-formyl-6-methylpyrene + NADH + H+
-
-
-
r
1-hydroxymethyl-8-methylpyrene + NAD+
1-formyl-8-methylpyrene + NADH + H+
-
-
-
r
1-hydroxymethylpyrene + NAD+
1-formylpyrene + NADH + H+
-
-
-
r
1-indanol + NAD+
1-indanone + NADH + H+
1-indanone + NADH + H+
(S)-1-indanol + NAD+
1-indanone + NADH + H+
1-indanol + NAD+
-
-
-
-
r
1-nonanol + NAD+
nonanal + NADH + H+
1-octanol + 2 NAD+ + H2O
octanoic acid + 2 NADH + 2 H+
1-octanol + 2 NADP+ + H2O
octanoic acid + 2 NADPH + 2 H+
-
-
-
r
1-octanol + NAD+
1-octanal + NADH + H+
-
6% of activity with N-benzyl-3-pyrrolidinol
-
-
?
1-octanol + NAD+
octanal + NADH
1-octanol + NAD+
octanal + NADH + H+
1-pentanol + NAD+
1-pentanal + NADH + H+
1-pentanol + NAD+
pentanal + NADH + H*
-
8% of the activity with (S)-N-benzyl-3-pyrrolidinol
-
-
?
1-pentanol + NAD+
pentanal + NADH + H+
1-phenyl-1,2-ethanediol + NAD+
1-phenyl-2-propanone + NADH + H+
1% activity compared to cyclohexanol
-
-
r
1-phenyl-1,2-propandione + NADH + H+
1-phenyl-2-hydroxy-1-propanone + NAD+
-
146% of the activity with 2,2,2-trifluoroacetophenone
-
-
r
1-phenyl-1,2-propanedione + NADH
?
about 25% of the activity compared to isatin
-
-
?
1-phenyl-1,2-propanedione + NADH + H+
?
1-phenyl-1-propanol + NAD+
1-phenyl-1-propanone + NADH + H+
-
59% of the activity with (S)-(-)-1-phenylethanol
-
-
?
1-phenyl-1-propanol + NAD+
1-phenylpropanal + NADH + H+
-
59% of the activity with (S)-1-phenylethanol
-
-
r
1-phenyl-1-propanol + NAD+
?
-
31% of the activity with (S)-N-benzyl-3-pyrrolidinol
-
-
?
1-phenyl-2-butanone + NADH + H+
1-phenylbutan-2-ol + NAD+
1-phenyl-2-propanol + NAD+
1-phenyl-2-propanone + NADH + H+
1-phenyl-2-propanone + NADH + H+
(S)-1-phenyl-2-propanol + NAD+
-
-
-
-
-
1-phenyl-3-butanone + NADH + H+
1-phenylbutan-3-ol + NAD+
-
353% of the activity with phenyl trifluoromethyl ketone
-
-
?
1-phenylethanol + NAD+
1-phenylethanone + NADH
1-phenylethanol + NAD+
1-phenylethanone + NADH + H+
1-phenylethanol + NAD+
acetophenone + NADH + H+
-
1% activity compared to cyclohexanone
-
?
1-phenylethanol + NADP+
1-phenylethanone + NADPH + H+
-
-
-
?
1-propanol + NAD+
propanal + NADH
-
-
-
-
?
1-propanol + NAD+
propanal + NADH + H+
1-propanol + NAD+
propanaldehyde + NADH + H+
11-cis-retinal + NADH + H+
11-cis-retinol + NAD+
-
-
-
-
?
11-cis-retinol + NAD+
11-cis-retinal + NADH
12-hydroxydodecanoate + NAD+
12-oxododecanoic acid + NADH
12-hydroxylauric acid methyl ester + NAD+
12-oxo lauricacid methyl ester + NADH + H+
-
-
product is a key intermediate for biobased polyamide 12 production
-
?
12-oxolauric acid methyl ester + NADH + H+
12-hydroxylauric acid methyl ester + NAD+
-
-
-
-
?
13-cis-retinal + NADH + H+
13-cis-retinol + NAD+
-
-
-
-
?
13-cis-retinol + NAD+
13-cis-retinal + NADH
16-hydroxyhexadecanoate + NAD+
16-oxohexadecanoic acid + NADH
-
-
-
-
?
17beta-hydroxyetiocholan-3-one + NAD+
ethiocholan-3,17-dione + NADH
-
-
-
-
?
2',3',4',5',6'-pentafluoroacetophenone + NADH + H+
1-(2,3,4,5,6-pentafluorophenyl)ethanol + NAD+
45% of the activity compared to 1-phenyl-1,2-propanedione
-
-
?
2,2'-dichlorobenzil + NADH + H+
1,2-bis(2-chlorophenyl)-2-hydroxyethanone + NAD+
-
-
-
?
2,2,2-trichloroethanol + NAD+
trichloroacetaldehyde + NADH + H+
-
-
-
-
?
2,2,2-trifluoroacetophenone + NADH
2,2,2-trifluoro-1-phenylethanol + NAD+
about 35% of the activity compared to isatin
-
-
?
2,2,2-trifluoroacetophenone + NADH + H+
(R)-alpha-(trifluoromethyl)benzyl alcohol + NAD+
2,2,2-trifluoroacetophenone + NADH + H+
2,2,2-trifluoro-1-phenylethanol + NAD+
2,2,2-trifluoroacetophenone + NADPH + H+
(R)-2,2,2-trifluoro-1-phenylethanol + NADP+
180% of the activity with acetoin
-
-
r
2,2-dichloroacetophenone + NADH + H+
2,2-dichloro-1-phenylethanol + NAD+
2,3'-dichloroacetophenone + NADH + H+
1-(2,3-dichlorophenyl)ethanol + NAD+
-
67% of the activity with phenyl trifluoromethyl ketone
-
-
?
2,3'-dichloroacetophenone + NADH + H+
2-chloro-1-(3-chlorophenyl)ethanol + NAD+
-
-
-
-
r
2,3-butanediol + H2O
?
-
-
-
?
2,3-butanediol + NAD+
?
-
83% of the activity with (S)-N-benzyl-3-pyrrolidinol
-
-
?
2,3-butanediol + NAD+
acetoin + NADH + H+
2,3-butanedione + NADH + H+
?
-
activity is 1.6fold higher than with N-benzyl-3-pyrrolidinone
-
-
?
2,3-pentanedione + NADH
? + NADH
-
5.5% of the activity with acetaldehyde
-
-
?
2-(3,5-dimethylphenyl)propanal + NADH + H+
(S)-2-(3,5-dimethylphenyl)propanol + NAD+
2-(3-benzoylphenyl)propanal + NADH + H+
(S)-2-(3-benzoylphenyl)propanol + NAD+
2-(3-fluorobiphenyl-4-yl)propanal + NADH + H+
(S)-2-(3-(fluoro)biphenyl-4-yl)propanol + NAD+
2-(3-phenoxyphenyl)propanal + NADH + H+
(S)-2-((3-phenoxy)phenyl)propanol + NAD+
2-(4-isobutylphenyl)propanal + NADH + H+
(S)-2-(4-isobutylphenyl)propanol + NAD+
2-(4-trifluoromethylphenyl)propanal + NADH + H+
(S)-2-(4-trifluoromethyl)phenylpropanol + NAD+
-
18 h, 55% yield, 98% enantiomeric excess
-
-
?
2-(6-methoxynaphthalen-2-yl)propanal + NADH + H+
(S)-2-(6-methoxynaphthalen-2-yl)propan-1-ol + NAD+
-
18 h, 96% yield, 98% enantiomeric excess
-
-
?
2-(naphthalen-1-yl)propanal + NADH + H+
(S)-2-(naphthalen-1-yl)propanol + NAD+
-
18 h, 90% yield, 80% enantiomeric excess
-
-
?
2-(naphthalen-2-yl)propanal + NADH + H+
(S)-2-(naphthalen-2-yl)propanol + NAD+
-
18 h, 57% yield, 94% enantiomeric excess
-
-
?
2-acetylcyclohexanone + NADH + H+
2-acetylcyclohexanol + NAD+
-
-
-
-
r
2-acetylcyclopentanone + NADH + H+
2-acetylcyclopentanol + NAD+
-
-
-
-
r
2-acetylfuran + NADH + H+
? + NAD+
-
-
-
-
r
2-acetylpyridine + NADH + H+
(R)-1-(2-pyridyl)ethanol + NAD+
-
-
-
-
r
2-acetylpyrrole + NADH + H+
1-(1H-pyrrol-2-yl)ethanol + NAD+
-
70% of activity with N-benzyl-3-pyrrolidinone
-
-
?
2-acetylpyrrole + NADH + H+
? + NAD+
-
-
-
-
r
2-acetylthiazole + NADH + H+
? + NAD+
-
-
-
-
r
2-acetylthiophene + NADH + H+
? + NAD+
-
-
-
-
r
2-aminoethanol + NAD+
?
-
-
-
?
2-butanol + NAD+
2-butanone + NADH + H+
2-butanone + NADH + H+
2-butanol + NAD+
2-butanone + NADH + H+
butan-2-ol + NAD+
-
2.8fold higher activity compared to activity with N-benzyl-3-pyrrolidinone
-
-
?
2-butanone + NADPH + H+
butan-2-ol + NADP+
2-butene-1-ol + NAD+
? + NADH
2-chloroacetophenone + NADH + H+
1-(2-chlorophenyl)ethanol + NAD+
-
low activity
-
-
r
2-chlorocyclohexanone + NADH + H+
? + NAD+
3% activity compared to cyclohexanone
-
-
?
2-decalone + NADH + H+
? + NAD+
28% activity compared to cyclohexanone
-
-
?
2-decanol + NAD+
2-decanone + NADH + H+
weak activity
-
-
r
2-decanone + NADH
(S)-2-decanol + NAD+ + H+
-
-
92% enantiomeric excess
-
?
2-deoxy-D-ribose + NAD+
? + NADH
-
-
-
-
?
2-ethoxyethanol + NAD+
2-ethoxyacetaldehyde + NADH + H+
-
-
-
-
r
2-ethylhexan-1-ol + NAD+
2-ethylhexanal + NADH
Sporotrichum pulverulentum
-
weak activity
-
-
?
2-heptanol + NAD+
2-heptanone + NADH + H+
2-heptanol + NAD+
heptan-2-one + NADH + H+
62% of the activity with 1-phenylethanol
-
-
?
2-heptanone + NADH
(S)-2-heptanol + NAD+ + H+
-
-
79% enantiomeric excess
-
?
2-heptanone + NADH + H+
(R)-2-heptanol + NAD+
-
229% of the activity with phenyl trifluoromethyl ketone
99% enantiomeric excess
-
?
2-heptanone + NADPH + H+
heptan-2-ol + NADP+
2-hexanol + NAD+
2-hexanone + NADH + H+
2-hexanone + NADH
(S)-2-hexanol + NAD+ + H+
-
-
37% enantiomeric excess
-
?
2-hexanone + NADH + H+
2-hexanol + NAD+
-
-
-
-
r
2-hexanone + NADH + H+
?
-
activity is 1.6fold higher than with N-benzyl-3-pyrrolidinone
-
-
?
2-hexanone + NADH + H+
hexan-2-ol + NAD+
-
50.4fold higher activity compared to activity with N-benzyl-3-pyrrolidinone
-
-
?
2-hexanone + NADPH + H+
hexan-2-ol + NADP+
2-hydroxyacetophenone + NADH + H+
(S)-1-phenyl-1,2-ethanediol + NAD+
2-hydroxyacetophenone + NADPH + H+
(S)-1-phenyl-1,2-ethanediol + NADP+
-
-
-
?
2-hydroxymethylpyrene + NAD+
2-formylpyrene + NADH + H+
-
-
-
r
2-mercaptoethanol + NAD+
?
2-methoxybenzaldehyde + NADH + H+
2-methoxybenzylalcohol + NAD+
-
13% of the activity with 2,2,2-trifluoroacetophenone
-
-
?
2-methoxybenzyl alcohol + NAD+
2-methoxybenzaldehyde + NADH + H+
-
25% of the activity with (S)-(-)-1-phenylethanol
-
-
?
2-methoxyethanol + NAD+
2-methoxyacetaldehyde + NADH + H+
-
-
-
r
2-methyl-2,4-pentanediol + NAD+
?
1% activity compared to cyclohexanol
-
-
?
2-methylbutan-1-ol + NAD+
? + NADH
-
-
-
-
?
2-methylbutyraldehyde + NADH + H+
2-methylbutanol + NAD+
-
1.4% of the activity with acetaldehyde
-
-
?
2-methylcyclohexanone + NADH + H+
2-methylcyclohexanol + NAD+
13% of the activity compared to 1-phenyl-1,2-propanedione
-
-
?
2-methylcyclohexanone + NADH + H+
? + NAD+
46% activity compared to cyclohexanone
-
-
?
2-methylpropan-1-ol + NAD+
2-methyl-propan-1-one + NADH
2-methylpropan-1-ol + NAD+
2-methyl-propan-1-one + NADH + H+
2-methylpropionaldehyde + NADH + H+
2-methylpropanal + NAD+
-
3.3% of the activity with acetaldehyde
-
-
?
2-nitrobenzaldehyde + NADH + H+
(2-nitrophenyl)methanol + NAD+
-
33% of activity with N-benzyl-3-pyrrolidinone
-
-
?
2-nitrobenzaldehyde + NADH + H+
2-nitrobenzyl alcohol + NAD+
-
low activity
-
-
r
2-nonanol + NAD+
2-nonanone + NADH + H+
weak activity
-
-
r
2-nonanone + NADH
(S)-2-nonanol + NAD+ + H+
-
-
95% enantiomeric excess
-
?
2-octanol + NAD+
2-octanone + NADH + H+
2-octanone + NADH
(S)-2-octanol + NAD+ + H+
-
-
92% enantiomeric excess
-
?
2-octanone + NADH + H+
2-octanol + NAD+
-
-
-
-
r
2-octanone + NADPH + H+
octan-2-ol + NADP+
11.9% of the activity with 2-hydroxyacetophenone and NADPH
-
-
?
2-oxo-3-methylpentane + NADH
3-methylpentan-2-ol + NAD+
2-oxo-3-phenylpropane + NADH
2-hydroxy-3-phenylpropane + NAD+
2-oxo-4-methylpentane + NADH
4-methylpentan-2-ol + NAD+
-
-
-
-
?
2-oxo-butyric acid + NADH + H+
2-hydroxybutyric acid + NAD+
-
15% of activity with N-benzyl-3-pyrrolidinone
-
-
?
2-oxohexane + NADH
2-hydroxyhexane + NAD+
-
-
-
-
?
2-oxopentane + NADH
2-pentanol + NAD+
-
-
-
-
?
2-oxopentanoate + NADH + H+
2-hydroxypentanoate + NAD+
-
low activity
-
-
r
2-oxopropanal + NADH + H+
? + NAD+
328% of the activity with acetoin
-
-
r
2-pentanol + NAD+
2-pentanone + NADH + H+
2-pentanone + NADH
(S)-2-pentanol + NAD+ + H+
-
-
60% enantiomeric excess
-
?
2-pentanone + NADH
pentan-2-ol + NAD+
-
-
-
r
2-pentanone + NADH + H+
2-pentanol + NAD+
2-pentanone + NADPH + H+
pentan-2-ol + NADP+
11.9% of the activity with 2-hydroxyacetophenone and NADPH
-
-
?
2-phenylcyclohexanone + NADH + H+
? + NAD+
2% activity compared to cyclohexanone
-
-
?
2-phenylethanol + NAD+
2-phenylethanone + NADH + H+
57% activity compared to cyclohexanol
-
-
r
2-phenylethanol + NAD+
phenylacetaldehyde + NADH + H+
-
-
-
-
r
2-phenylpropanal + NADH + H+
(S)-2-phenylpropanol + NAD+
-
18 h, 74% yield, 98% enantiomeric excess
-
-
?
2-propanol + NAD(P)+
acetone + NAD(P)H
2-propanol + NAD+
2-propanone + NADH
2-propanol + NAD+
2-propanone + NADH + H+
2-propanol + NAD+
aceton + NADH + H+
-
-
irreversible, no measurable activity with acetone
-
ir
2-propanol + NAD+
acetone + NADH
-
-
-
-
?
2-propanol + NAD+
acetone + NADH + H+
2-propanol + NAD+
propanone + NADH + H+
-
-
-
-
r
2-[3-(2-phenyl-1,3-dioxolan-2-yl)phenyl]propanal + NADH + H+
(S)-2-(3-(2-phenyl-1,3-dioxolan-2-yl)phenyl)propanol + NAD+
-
18 h, 95% yield, 61% enantiomeric excess
-
-
?
3',4'-dimethoxyphenylacetone + NADH + H+
1-(3,4-dimethoxyphenyl)propan-2-ol + NAD+
-
24% of the activity with phenyl trifluoromethyl ketone
-
-
?
3'-bromoacetophenone + NADH + H+
1-(3-bromophenyl)ethanol + NAD+
-
151% of the activity with phenyl trifluoromethyl ketone
-
-
?
3'-chloroacetophenone + NADH + H+
1-(3-chlorophenyl)ethanol + NAD+
-
70% of the activity with phenyl trifluoromethyl ketone
-
-
?
3'-methoxyacetophenone + NADH + H+
1-(3-methoxyphenyl)ethanol + NAD+
-
51% of the activity with phenyl trifluoromethyl ketone
-
-
?
3,3,5-trimethylcyclohexanone + NADH + H+
? + NAD+
2% activity compared to cyclohexanone
-
-
?
3,4-dihydro-retinol + NAD+
3,4-dihydro-retinal
-
-
-
-
r
3,4-dimethylbenzyl alcohol + NAD+
3,4-dimethylbenzaldehyde + NADH + H+
45% activity compared to cyclohexanol
-
-
r
3,4-hexanedione + NADH + H+
?
-
77% of the activity with N-benzyl-3-pyrrolidinone
-
-
?
3,4-methylenedioxyphenyl acetone + NADH
(S)-(3,4-methylenedioxyphenyl)-2-propanol + NAD+ + H+
-
-
-
-
-
3,5-dimethylcyclohexanol + NAD+
3,5-dimethylcyclohexanone + NADH + H+
1% activity compared to cyclohexanol
-
-
r
3-acetylpyridine + NADH + H+
(R)-1-(3-pyridyl)ethanol + NAD+
-
-
-
-
r
3-acetylpyridine + NADH + H+
1-pyridin-3-ylethanol + NAD+
-
7.9fold higher activity compared to activity with N-benzyl-3-pyrrolidinone
-
-
?
3-aminobenzyl alcohol + NAD+
3-aminobenzaldehyde + NADH + H+
8% activity compared to cyclohexanol
-
-
r
3-bromobenzyl alcohol + NAD+
3-bromobenzaldehyde + NADH + H+
-
-
-
-
?
3-bromobenzylalcohol + NAD+
3-bromobenzaldehyde + NADH + H+
-
-
-
-
?
3-butene-1-ol + NAD+
? + NADH
-
-
-
-
?
3-chloro-2-butanone + NADH + H+
3-chlorobutan-2-ol + NAD+
-
151% of the activity with phenyl trifluoromethyl ketone
-
-
?
3-chlorobenzaldehyde + NADH + H+
3-chlorobenzyl alcohol + NAD+
-
-
-
-
r
3-heptanol + NAD+
3-heptanone + NADH + H+
-
93% of the activity with 2-propanol
-
-
r
3-methoxy-1-phenylpropan-1-one + NADH
3-methoxy-1-phenylpropan-1-ol + NAD+
-
-
-
-
?
3-methoxybenzaldehyde + NADH + H+
3-methoxybenzyl alcohol + NAD+
-
-
-
-
r
3-methoxybenzaldehyde + NADH + H+
3-methoxybenzylalcohol + NAD+
-
14% of the activity with 2,2,2-trifluoroacetophenone
-
-
?
3-methoxybenzyl alcohol + NAD+
3-methoxybenzaldehyde + NADH + H+
3-methoxybenzylalcohol + NAD+
3-methoxybenzaldehyde + NADH + H+
-
-
-
-
?
3-methyl-1-butanol + NAD+
? + NADH
-
-
-
-
?
3-methyl-2-cyclohexenone + NADH + H+
? + NAD+
1% activity compared to cyclohexanone
-
-
?
3-methylbutan-1-ol + NAD+
3-methyl-butan-1-one + NADH + H+
3-methylbutan-2-ol + NAD+
3-methylbutan-2-one + NADH
-
R-(-)-3-methylbutan-2-ol and S-(+)-3-methylbutan-2-ol
-
-
?
3-methylbutan-2-one + NADH
3-methylbutan-2-ol + NAD+
-
-
-
-
?
3-methylbutan-2-one + NADH + H+
3-methyl-2-butanol + NAD+
-
-
-
-
r
3-methylbutan-2-one + NADH + H+
3-methylbutan-2-ol + NAD+
-
-
-
-
?
3-methylbutanal + NAD+
? + NADH + H+
3-methylbutanol + NAD+
3-methylbutanone + NADH + H+
133% activity compared to cyclohexanol
-
-
r
3-methylbutanol + NAD+
? + NADH
3-methylbutyraldehyde + NADH + H+
3-methylbutanol + NAD+
-
2.9% of the activity with acetaldehyde
-
-
?
3-methylcyclohexanol + NAD+
3-methylcyclohexanone + NADH
-
-
-
-
r
3-methylcyclohexanol + NAD+
3-methylcyclohexanone + NADH + H+
14% of activity compared to (S)-1-indanol
-
-
?
3-methylcyclohexanol + NAD+
?
10% of the activity compared to isoborneol
-
-
?
3-methylcyclohexanone + NADH
3-methylcyclohexanol + NAD+
3-methylcyclohexanone + NADH + H+
3-methylcyclohexanol + NAD+
33% of the activity compared to 1-phenyl-1,2-propanedione
-
-
?
3-methylphenylethyl alcohol + NAD+
?
64% activity compared to cyclohexanol
-
-
?
3-nitroacetophenone + NADH + H+
1-(3-nitrophenyl)ethanol + NAD+
-
-
-
-
r
3-nitrobenzaldehyde + NADH + H+
3-nitrobenzyl alcohol + NAD+
3-oxo-5beta-androstan-17beta-ol + NADH
3beta,17beta-dihydroxy-5beta-androstane + NAD+
3-oxoheptane + NADH
3-hydroxyheptane + NAD+
-
-
-
-
?
3-pentanol + NAD+
3-pentanone + NADH + H+
3-pentanone + NADH
pentan-3-ol + NAD+
-
-
-
r
3-pentanone + NADPH + H+
pentan-3-ol + NADP+
7.1% of the activity with 2-hydroxyacetophenone and NADPH
-
-
?
3-penten-2-one + NADH + H+
? + NAD+
2% activity compared to cyclohexanone
-
-
?
3-phenyl-1-propanol + NAD+
3-phenyl-1-propanone + NADH
3-phenyl-2-propen-1-ol + NAD+
? + NADH
-
-
-
-
?
3-phenylpropanol + NAD+
3-phenylpropanal + NADH + H+
-
135% of the activity with 2-phenylethanol
-
-
?
3-phenylpropionaldehyde + NADH
3-phenylpropan-1-ol + NAD+
-
1218% of the activity with phenyl trifluoromethyl ketone
-
-
?
3-phenylpropionaldehyde + NADH + H+
3-phenylpropanol + NAD+
-
6.9fold higher activity compared to activity with N-benzyl-3-pyrrolidinone
-
-
?
3-pyridylcarbinol + NAD+
pyridine-3-carbaldehyde + NADH
-
-
-
-
?
3beta,12alpha-dihydroxy-5beta-cholanoic acid + NAD+
? + NADH
-
-
-
-
?
3beta,17beta-dihydroxy-5beta-androstane + NAD+
5beta-androstan-3,17dione + NADH
-
-
-
-
?
3beta,7alpha,12alpha-trihydroxy-5beta-cholanoic acid + NAD+
? + NADH
-
-
-
-
?
3beta,7alpha-dihydroxy-5beta-cholanoic acid + NAD+
? + NADH
-
-
-
-
?
3beta-hxdroxy-5alpha-cholanoate + NAD+
3-oxo-5alpha-cholanoate + NADH
-
-
-
-
?
3beta-hydroxy-5beta-androstan-17-one + NAD+
5beta-androstan-3,17-dione + NADH
3beta-hydroxy-5beta-cholanoate + NAD+
3-oxo-5beta-cholanoate + NADH
-
-
-
-
?
4'-bromoacetophenone + NADH + H+
1-(4-bromophenyl)ethanol + NAD+
-
77% of the activity with phenyl trifluoromethyl ketone
-
-
?
4'-chloroacetophenone + NADH + H+
1-(4-chlorophenyl)ethanol + NAD+
-
60% of the activity with phenyl trifluoromethyl ketone
-
-
?
4'-chlorobutyrophenone + NADH + H+
1-(4-chlorophenyl)butan-1-ol + NAD+
-
10% of the activity with 2,2,2-trifluoroacetophenone
-
-
?
4-acetylpyridine + NADH + H+
(R)-1-(4-pyridyl)ethanol + NAD+
-
-
-
-
r
4-acetylpyridine + NADH + H+
1-pyridin-4-ylethanol + NAD+
-
64.5fold higher activity compared to activity with N-benzyl-3-pyrrolidinone
-
-
?
4-bromobenzyl alcohol + NAD+
4-bromobenzaldehyde + NADH + H+
-
-
-
-
?
4-bromobenzylalcohol + NAD+
4-bromobenzaldehyde + NADH + H+
-
-
-
-
?
4-carboxybenzaldehyde + NADH + H+
4-carboxybenzyl alcohol + NAD+
-
-
-
-
?
4-carboxybenzaldehyde + NADH + H+
4-carboxybenzylalcohol + NAD+
-
-
-
-
?
4-chloroacetophenone + NADH
1-(4-chlorophenyl)ethanol + NAD+
4-chloroacetophenone + NADH + H+
1-(4-chlorophenyl)ethanol + NAD+
-
-
-
-
r
4-chlorobenzaldehyde + NADH + H+
4-chlorobenzyl alcohol + NAD+
-
-
-
-
r
4-ethylcyclohexanol + NAD+
4-ethylcyclohexanone + NADH + H+
60% activity compared to cyclohexanol
-
-
r
4-ethylcyclohexanone + NADH + H+
? + NAD+
22% activity compared to cyclohexanone
-
-
?
4-fluoroacetophenone + NADH + H+
1-(4-fluorophenyl)ethanol + NAD+
-
-
-
-
r
4-hydroxy-2-butanone + NADH + H+
2,4-dihydroxybutane + NAD+
-
26% of the activity with phenyl trifluoromethyl ketone
-
-
?
4-hydroxy-retinol + NAD+
4-oxo-retinal + NADH
-
-
-
-
r
4-hydroxymethylpyrene + NAD+
4-formylpyrene + NADH + H+
-
-
-
r
4-hydroxynonenal + NADH + H+
4-hydroxynonenol + NAD+
-
substrate of isozyme ADH4
-
-
r
4-methoxy-1-naphthaldehyde + NAD+
4-methoxy-1-naphthol + NADH + H+
-
fluorogenic substrate of class I and II isozymes
-
-
?
4-methoxy-1-naphthaldehyde + NAD+
4-methoxy-1-naphthyl alcohol + NADH + H+
-
substrate for class I ADH
-
-
?
4-methoxy-1-naphthaldehyde + NADH + H+
4-methoxynaphthalene-1-carbaldehyde + NAD+
-
substrate for class I ADH
-
-
r
4-methoxybenzaldehyde + NADH + H+
4-methoxybenzyl alcohol + NAD+
-
-
51% of the activity with butan-2-ol
-
?
4-methoxybenzaldehyde + NADH + H+
4-methoxybenzylalcohol + NAD+
-
13% of the activity with 2,2,2-trifluoroacetophenone
-
-
?
4-methoxybenzyl alcohol + NAD+
4-methoxybenzaldehyde + NADH + H+
4-methoxybenzylalcohol + NAD+
4-methoxybenzaldehyde + NADH + H+
-
-
-
-
r
4-methoxyphenylacetone + NADH
(2S)-1-(4-methoxyphenyl)propan-2-ol + NAD+ + H+
-
-
-
-
?
4-methyl-1-pentanol + NAD+
4-methyl-1-pentanal + NADH
4-methylcyclohexanol + NAD+
4-methylcyclohexanone + NADH + H+
56% activity compared to cyclohexanol
-
-
r
4-methylcyclohexanone + NADH + H+
4-methylcyclohexanol + NAD+
60% of the activity compared to 1-phenyl-1,2-propanedione
-
-
?
4-methylcyclohexanone + NADH + H+
? + NAD+
25% activity compared to cyclohexanone
-
-
?
4-methylpentan-2-one + NADH + H+
4-methyl-2-pentanol + NAD+
-
-
-
-
r
4-nitrobenzaldehyde + NADH + H+
4-nitrobenzyl alcohol + NAD+
4-nitrobenzaldehyde + NADH + H+
4-nitrobenzylalcohol + NAD+
-
-
-
-
?
4-nitrobenzyl alcohol + NAD+
4-nitrobenzaldehyde + NADH + H+
94% activity compared to benzyl alcohol
-
-
?
4-nitrosodimethylaniline + NAD+
? + NADH + H+
-
photometric assay substrate
-
-
?
4-phenylbutan-2-one + NADH
4-phenylbutan-2-ol + NAD+
-
-
-
-
?
5-acetyl-7-chlorofuro[2,3-c]pyridine + NADH + H+
5-(1-hydroxyethyl)-7-chlorofuro[2,3-c]pyridine + NAD+
-
-
-
-
r
5-acetylfuro[2,3-c]pyridine + NADH + H+
5-(1-hydroxyethyl)furo[2,3-c]pyridine + NAD+
-
-
-
-
r
5-hydroxymethylfurfural + NADH + H+
(furan-2,5-diyl)dimethanol + NAD+
mutant enzyme S109P/L116S/Y294C
-
-
?
5alpha-androstan-17beta-ol-3-one + NADH + H+
3beta,17beta-dihydroxy-5alpha-androstan + NAD+
5alpha-pregnan-3beta-ol-20-one + NAD+
5alpha-pregnan-3,20-dione + NADH
-
low activity
-
-
?
5beta-androstan-17beta-ol-3-one + NAD+
5beta-androstan-3,17-dione + NADH
5beta-androstan-3beta-ol-17-one + NAD+
5beta-androstan-3,17-dione + NADH
5beta-cholanic acid-3-one + NAD+
5beta-cholanic acid-3-ol + NAD+
-
-
-
-
?
5beta-cholanic acid-3-one + NADH
5beta-cholanic acid-3-ol + NAD+
-
low activity
-
-
?
5beta-pregnan-21-ol-3,20-dione hemisuccinate + NADH
5beta-pregnan-3,20,21-trione hemisuccinate + NADH
-
-
-
-
?
5beta-pregnan-3,20-dione + NADH
?
-
-
-
-
?
5beta-pregnan-3beta-ol-20-one + NAD+
5beta-pregnan-3,20-dione + NADH
-
-
-
-
?
6-benzyloxy-3,5-dioxo-hexanoic acid ethyl ester + NADH + H+
(3R,5S)-6-benzyloxy-3,5-dihydroxy-hexanoic acid ethyl ester + NAD+
-
-
-
-
-
6-methoxy-2-naphthaldehyde + NADH + H+
(6-methoxynaphthalen-2-yl)methanol + NAD+
-
substrate for class II ADH
-
-
r
6-methoxy-2-naphthaldehyde + NADH + H+
6-methoxy-2-naphthyl alcohol + NAD+
-
substrate for class II ADH
-
-
?
6-methoxy-2-naphthaldehyde + NADH + H+
6-methoxy-2-naphtol + NAD+
-
class II isozyme, reductive activity
-
-
?
7-cis-retinol + NAD+
7-cis-retinal + NADH
9-cis-retinol + NAD+
9-cis-retinal + NADH
a primary alcohol + NAD+
an aldehyde + NADH + H+
-
ADH3 is involved in multiple cellular pathways, as diverse as formaldehyde detoxification, retinoid metabolism and NO homeostasis, ADH3 is considered to play only a minor role in hepatic alcohol metabolism because ethanol concentrations rarely exceed 50 mM
-
-
?
acetaldehyde + NADH + H+
ethanol + NAD+
acetaldehyde + NADH + H+
ethanol + NAD+ + H+
acetaldehyde + NADPH + H+
ethanol + NADP+
acetoin + NAD+
diacetyl + NADH
18% of the activity with 2,3-butanediol
-
-
?
acetoin + NADH + H+
2,3-butanediol + NAD+
-
-
-
r
acetoin + NADH + H+
3-hydroxy-2-butanol + NAD+
-
-
-
-
r
acetoin + NADH + H+
butan-2,3-diol + NAD+
-
-
-
?
acetone + NAD+ + H2O
? + NADH + H+
about 5% of the activity with 1-octanol
-
-
r
acetone + NADH
isopropanol + NAD+
acetone + NADH + H+
2-propanol + NAD+
-
-
-
-
?
acetone + NADH + H+
propan-2-ol + NAD+
-
1.9fold higher activity compared to activity with N-benzyl-3-pyrrolidinone
-
-
?
acetone + NADPH + H+
propan-2-ol + NADP+
14.7% of the activity with 2-hydroxyacetophenone and NADPH
-
-
?
acetophenone + NADH
(R)-alpha-phenyl ethanol + NAD+
-
the enzyme exhibits high stereoselectivity in the desymmetrization of the prochiral ketone acetophenone, producing optically pure (R)-alpha-phenyl ethanol at high conversion
-
-
?
acetophenone + NADH + H+
(R)-1-phenylethanol + NAD+
-
6% of the activity with phenyl trifluoromethyl ketone, in the reverse reaction 87% of the activity with 2-propanol
99% enantiomeric excess
-
?
acetophenone + NADH + H+
(S)-(-)-1-phenylethanol + NAD+
-
more than 99% enantiomeric excess
-
-
r
acetophenone + NADH + H+
(S)-1-phenylethanol + NAD+
-
-
99% enantiomeric excess
-
r
acetophenone + NADH + H+
1-phenylethanol + NAD+
acetophenone + NADPH + H+
1-phenylethanol + NADP+
acetylacetone + 2 NADH + 2 H+
2,4-pentanediol + 2 NAD+
-
-
-
-
r
acetylacetone + NADH + H+
?
-
63% of the activity with N-benzyl-3-pyrrolidinone
-
-
?
acetylacetone + NADH + H+
acetophenone + NADH + H+
1% activity compared to cyclohexanone
1% activity compared to cyclohexanone
-
r
acetylpyrazine + NADH + H+
(R)-1-(pyrazyl)ethanol + NAD+
-
-
-
-
r
alcohol + NAD+
aldehyde or ketone + NADH
all-trans-retinal + NADH + H+
all-trans-retinol + NAD+
-
-
-
-
?
all-trans-retinol + NAD+
all-trans-retinal + NADH
allyl alcohol + NAD+
acrolein + NADH
allyl alcohol + NAD+
prop-2-en-1-al + NADH
allylalcohol + NAD+
prop-2-enal + NADH + H+
-
-
-
?
alpha-ethyl benzoylformate + NADH + H+
ethyl (R)-()-mandelate + NAD+
-
-
95% enantiomeric excess
-
r
alpha-methyl benzoylformate + NADH + H+
methyl (R)-mandelate + NAD+
-
-
92% enantiomeric excess
-
r
alpha-tetralone + NADH + H+
(S)-alpha-tetralol + NAD+
alpha-tetralone + NADH + H+
alpha-tetralol + NAD+
-
-
-
-
r
anisaldehyde + NADH
anisic alcohol + NADH
-
-
-
-
?
anisaldehyde + NADH + H+
anisic alcohol + NAD+
-
-
-
-
?
benzaldehyde + NADH
benzyl alcohol + NAD+
benzaldehyde + NADH + H+
benzyl alcohol + NAD+
benzaldehyde + NADPH + H+
benzyl alcohol + NADP+
benzil + NADH + H+
(R)-benzoin + NAD+
62% of the activity compared to 1-phenyl-1,2-propanedione. The enzyme catalyses the asymmetric reduction of benzil to (R)-benzoin with both excellent conversion (98%) and optical purity (98%) by way of an efficient in situ NADH-recycling system involving a second thermophilic ADH
-
-
?
benzoin + NADH + H+
1,2-diphenylethane-1,2-diol
-
13% of activity with N-benzyl-3-pyrrolidinone
-
-
?
benzoylformic acid + NADPH + H+
mandelate + NADP+
2.4% of the activity with 2-hydroxyacetophenone and NADPH
-
-
?
benzyl aclohol + NAD+
benzaldehyde + NADH + H+
-
-
-
-
?
benzyl alcohol + NAD+
benzaldehyde + NADH
benzyl alcohol + NAD+
benzaldehyde + NADH + H+
benzyl alcohol + NADP+
benzaldehyde + NADPH + H+
benzylacetone + NADH + H+
4-phenylbutan-2-ol + NAD+
benzylalcohol + NAD+
benzaldehyde + NADH + H+
benzyloxycarbonyl-3-pyrrolidinone + NADH + H+
(S)-benzyloxycarbonyl-3-pyrrolidinol + NAD+
-
production with 99.6% enantiomeric excess
-
-
?
butan-2,3-diol + NAD+
acetoin + NADH + H+
-
-
-
?
butan-2-ol + NAD+
butan-2-one + NADH
butan-2-ol+ NAD+
butanone + NADH + H+
-
-
83% of the activity with butan-2-ol
-
?
butan-2-one + NADH
butan-2-ol + NAD+
butanal + NADH
1-butanol + NAD+ + H+
-
% of the activity with phenyl trifluoromethyl ketone
-
-
ir
butanol + NAD+
1-butanal + NADH + H+
butanol + NAD+
butanal + NADH
-
-
-
-
?
butanol + NAD+
butyraldehyde + NADH
butanol + NAD+
butyraldehyde + NADH + H+
butanol + NAD+
n-butanal + NADH
-
-
-
?
butyraldehyde + NAD+
n-butanol + NADH + H+
-
-
-
-
r
butyraldehyde + NADH
n-butanol + NAD+
butyraldehyde + NADH + H+
butanol + NAD+
-
56% of the activity with acetaldehyde
-
-
?
butyraldehyde + NADH + H+
n-butanol + NAD+
butyraldehyde + NADPH + H+
butanol + NADP+
-
22% of the activity with NADH
-
-
?
capronaldehyde + NADH
1-hexanol + NAD+
chloroacetone + NADH + H+
1-chloro-2-propanol + NAD+
-
-
-
-
r
chloroacetone + NADH + H+
1-chloropropan-2-ol + NAD+
choline + NAD+
? + NADH + H+
3% activity compared to benzyl alcohol
-
-
?
cinnamaldehyde + NADH + H+
cinnamyl alcohol + NAD+
-
-
-
-
r
cinnamyl alcohol + NAD+
cinnamaldehyde + NADH
cinnamyl alcohol + NAD+
cinnamaldehyde + NADH + H+
-
-
-
-
r
cis-4-methylcyclohexanol + NAD+
4-methylcyclohexanone + NADH
-
-
-
-
?
cis-decahydro-1-naphthol + NAD+
?
11% of activity compared to (S)-1-indanol
-
-
?
crotyl alcohol + NAD+
crotonaldehyde + NADH
cycloheptanol + NAD+
cycloheptanone + NADH + H+
cycloheptanone + NADH
cycloheptanol + NAD+
cyclohexanol + NAD+
cyclohexanone + NADH
cyclohexanol + NAD+
cyclohexanone + NADH + H+
cyclohexanone + NADH
cyclohexanol + NAD+
cyclohexanone + NADH + H+
?
-
activity is 2.1fold higher than with N-benzyl-3-pyrrolidinone
-
-
?
cyclohexanone + NADH + H+
cyclohexanol + NAD+
cyclooctanone + NADH
cyclooctanol + NAD+
-
-
-
-
?
cyclopentanol + NAD+
? + NADH
cyclopentanol + NAD+
cyclopentanone + NADH + H+
cyclopentanone + NADH
cyclopentanol + NAD+
-
-
-
-
?
cyclopentanone + NADH + H+
? + NAD+
1% activity compared to cyclohexanone
-
-
?
cyclopetanone + NADH
cyclopentanol + NADH
-
-
-
-
?
D-arabinose + NADH + H+
D-arabitol + NAD+
66% of the activity with 2,3-butanediol
-
-
?
D-glucitol + NAD+
? + NADH
decahydro-2-naphthol + NAD+
?
37% activity compared to cyclohexanol
-
-
?
decyl aldehyde + NADH + H+
1-decanol + NAD+
21% activity compared to benzyl alcohol
-
-
?
diacetyl + NADH + H+
2,3-butandiol + NAD+
-
-
-
-
r
diacetyl + NADH + H+
acetoin + NAD+
150% of the activity with acetoin
-
-
?
diethylketone + NADH + H+
3-pentanol + NAD+
-
-
-
-
r
digitose + NAD+
? + NADH
-
-
-
-
?
dihydro-4,4-dimethyl-2,3-furandione + NADH + H+
?
-
13.1fold higher activity compared to activity with N-benzyl-3-pyrrolidinone
-
-
?
dihydroxyacetone + NADH + H+
glycerol + NAD+
36% of the activity with acetoin
-
-
?
dihydroxyacetone phosphate + NADH + H+
glycerol phosphate + NAD+
82% of the activity with acetoin
-
-
?
DL-glyceraldehyde + H+
glycerol + NAD+
31% of the activity with acetoin
-
-
?
docosanol + NAD+
? + NADH + H+
-
immobilized HLAD shows about 60% reaction rate and free HLAD shows about 20% reaction rate with docosanol compared to ethanol at pH 8.8 and 30°C
-
-
?
dodecanol + NAD+
dodecanal + NADH + H+
-
immobilized HLAD shows about 60% reaction rate and free HLAD shows about 35% reaction rate with dodecanol compared to ethanol at pH 8.8 and 30°C
-
-
?
ethanol + 2 NAD+ + H2O
acetic acid + 2 NADH + 2 H+
ethanol + 2 NADP+ + H2O
acetic acid + 2 NADPH + 2 H+
-
-
-
r
ethanol + 3-benzoylpyridine-adenine dinucleotide
acetaldehyde + ?
-
rapid equilibrium bi bi mechanism
-
-
-
ethanol + beta-NAD+
acetaldehyde + NADH + H+
-
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH
ethanol + NAD+
acetaldehyde + NADH + H+
ethanol + NAD+
aldehyde + NADH + H+
-
immobilized HLAD shows 100% reaction rate and free HLAD shows about 55% reaction rate with ethanol at pH 8.8 and 30°C
-
-
?
ethanol + NADP+
acetaldehyde + NADPH + H+
ethyl 2-oxopropanoate + NADPH + H+
(R)-ethyl 2-hydroxypropanoate + NADP+
294& of the activity with acetoin
-
-
r
ethyl 3-methyl-2-oxobutyrate + NADH + H+
?
1-phenyl-1,2-propanedione and ethyl 3-methyl-2-oxobutyrate are the best substrate in the reduction reaction
-
-
?
ethyl 3-methyl-2-oxobutyrate + NADH + H+
ethyl-2-hydroxy-3-methylbutyrate + NAD+
-
33% of the activity with phenyl trifluoromethyl ketone
-
-
?
ethyl 3-oxobutanoate + NADH
ethyl 3-hydroxybutanoate + NAD+
-
-
-
-
?
ethyl 3-oxobutanoate + NADH + H+
ethyl 3-hydroxybutanoate + NAD+
-
309% of the activity with phenyl trifluoromethyl ketone
-
-
?
ethyl 3-oxobutyrate + NADH + H+
ethyl (S)-3-hydroxybutyrate + NAD+
-
-
-
-
-
ethyl 3-oxohexanoate + NADH
ethyl 3-hydroxyhexanoate + NAD+
-
-
-
-
?
ethyl 3-oxopentanoate + NADH
ethyl 3-hydroxypentanoate + NAD+
-
-
-
-
?
ethyl 3-phenyl-3-oxopropanoate + NADH
ethyl 3-phenyl-3-hydroxypropanoate + NAD+
-
-
-
-
?
ethyl 4-bromo-3-oxobutanoate + NADH + H+
ethyl 4-bromo-3-hydroxybutanoate
-
511% of the activity with phenyl trifluoromethyl ketone
-
-
?
ethyl 4-chloro-3-oxobutanoate + NADH + H+
ethyl 4-chloro-3-hydroxybutanoate + NAD+
-
809% of the activity with phenyl trifluoromethyl ketone
-
-
?
ethyl 4-chloroacetoacetate + NADH + H+
?
-
6.8fold higher activity compared to activity with N-benzyl-3-pyrrolidinone
-
-
?
ethyl 4-chloroacetoacetate + NADH + H+
ethyl 4-chloro-3-hydroxybutanoate + NAD+
-
-
-
-
r
ethyl 4-chloroacetoacetate + NADPH
?
activity is 1.48fold higher than with 2-hydroxyacetophenone and NADPH
-
-
?
ethyl acetoacetate + NADH + H+
?
-
42.4fold higher activity compared to activity with N-benzyl-3-pyrrolidinone
-
-
?
ethyl acetoacetate + NADH + H+
ethyl 3-hydroxybutanoate + NAD+
-
-
-
-
r
ethyl benzoylformate + NADH
?
about 30% of the activity compared to isatin
-
-
?
ethyl benzoylformate + NADH
ethyl (R)-mandelate + NAD+
-
enantiomeric excess of 99.9%
-
-
?
ethyl benzoylformate + NADH + H+
?
23% of the activity compared to 1-phenyl-1,2-propanedione
-
-
?
ethyl benzoylformate + NADH + H+
ethyl (R)-(-)-mandelate + NAD+
-
95% enantiomeric excess
-
-
?
ethyl benzoylformate + NADH + H+
ethyl (R)-mandelate + NAD+
ethyl oxo(phenyl)acetate + NADH + H+
ethyl hydroxy(phenyl)acetate + NAD+
-
100% of the activity with 2,2,2-trifluoroacetophenone
-
-
r
ethyl pyruvate + NADH
ethyl 2-hydroxypropanoate + NAD+
-
-
-
-
?
ethyl pyruvate + NADH + H+
ethyl 2-hydroxypropanoate + NAD+
-
488% of the activity with phenyl trifluoromethyl ketone
-
-
?
ethyl pyruvate + NADH + H+
ethyl 2-hydroxypropionate + NAD+
16% of the activity compared to 1-phenyl-1,2-propanedione
-
-
?
ethyl pyruvate + NADH + H+
ethyl lactate + NAD+
-
-
-
-
r
ethyl trifluoroacetoacetate + NADPH + H+
1-ethoxy-2,2,2-trifluoroethanol + NADP+
4.3% of the activity with 2-hydroxyacetophenone and NADPH
-
-
?
ethylene glycol + NAD+
? + NADH
ethylenglycol + NAD+
? + NADH
-
-
-
-
r
formaldehyde + NADH + H+
methanol + NAD+
furfural + NADH
furfuryl alcohol + NADH
-
activity with enzyme form ADH I, no activity with enzyme form ADH II
-
r
furfural + NADH + H+
furfuryl alcohol + NADH
mutant enzyme S109P/L116S/Y294C
-
-
?
furfuryl alcohol + NAD+
furfural + NADH
glutaraldehyde + NADH + H+
?
-
70.8fold higher activity compared to activity with N-benzyl-3-pyrrolidinone
-
-
?
glycerol + 2 NAD+ + H2O
? + 2 NADH + 2 H+
about 45% of the activity with ethanol
-
-
r
glycerol + NAD+
dihydroxyacetone + NADH
glycerol + NAD+
dihydroxyacetone + NADH + H+
-
-
-
-
?
heptaldehyde + NADH + H+
? + NAD+
33% activity compared to benzyl alcohol
-
-
?
heptan-2-ol + NAD+
heptan-2-one + NADH
-
-
-
-
?
heptan-4-ol + NAD+
heptan-4-one + NADH
-
-
-
-
?
hexadecanol + NAD+
hexadecanal + NADH
-
very low activity
-
-
?
hexadecanol + NAD+
hexadecanal + NADH + H+
-
immobilized HLAD shows about 60% reaction rate and free HLAD shows about 15% reaction rate with hexadecanol compared to ethanol at pH 8.8 and 30°C
-
-
?
hexaldehyde + NADH + H+
1-hexanol + NAD+
7% activity compared to benzyl alcohol
-
-
?
hexan-1-ol + NAD+
n-hexanal + NADH
hexan-2-ol + NAD+
2-hexanone + NADH
-
-
-
-
?
hexanal + NADH + H+
1-hexanone + NAD+
-
49% of the activity with acetaldehyde
-
-
?
hexanal + NADH + H+
hexan-1-ol + NAD+
16% of the activity with acetoin
-
-
?
hexanal + NADH + H+
n-hexanol + NAD+
-
22.3fold higher activity compared to activity with N-benzyl-3-pyrrolidinone
-
-
?
hexanal + NADPH + H+
1-hexanone + NADP+
-
24% of the activity with NADH
-
-
?
hexanol + NAD+
hexaldehyde + NADH
-
-
-
-
?
hexanol + NAD+
hexanal + NADH
-
-
-
-
?
hexanol + NAD+
n-hexanal + NADH
hydrocinnamaldehyde + NADH + H+
hydrocinnamyl alcohol + NAD+
12% activity compared to benzyl alcohol
-
-
r
hydrocinnamyl alcohol + NAD+
hydrocinnamaldehyde + NADH + H+
31% activity compared to benzyl alcohol
-
-
r
hydroxyacetone + NADH + H+
propane-1,2-diol + NAD+
-
27% of the activity with N-benzyl-3-pyrrolidinone
-
-
?
isatin + NADH + H+
? + NAD+
iso-propanol + NAD+
isopropanal + NADH + H+
isoamylalcohol + 2 NAD+ + H2O
? + 2 NADH + 2 H+
about 5% of the activity with 1-octanol
-
-
r
isoborneol + NAD+
?
isoborneol is the best substrate in the oxidation reaction
-
-
?
isobutyl alcohol + NAD+
? + NADH
-
-
-
-
?
isobutyraldehyde + NADH + H+
isobutanol + NAD+
-
-
-
-
r
isobutyramide + NAD+
?
-
-
-
-
r
isopentanol + NAD+
? + NADH + H+
low affinity
-
-
?
isopentenyl alcohol + NAD+
isopentanone + NADH
-
-
-
-
?
isopropanol + 2 NAD+ + H2O
? + 2 NADH + 2 H+
isopropanol + NAD+
2-propanone + NADH + H+
-
-
-
r
isopropanol + NAD+
acetone + NADH + H+
levulinic acid + NADH + H+
?
-
11% of activity with N-benzyl-3-pyrrolidinone
-
-
?
m-chlorophenacyl chloride + NADH + H+
2-chloro-1-(3-chlorophenyl)ethanol + NAD+
-
4.6fold higher activity compared to activity with N-benzyl-3-pyrrolidinone
-
-
?
m-nitrobenzaldehyde + NAD+
m-nitrobenzyl alcohol + NADH + H+
-
substrate of class IV ADH
-
-
?
m-nitrobenzaldehyde + NADH + H+
m-nitrobenzyl alcohol + NAD+
methanol + 2 NAD+ + H2o
formic acid + 2 NADH + 2 H+
about 25% of the activity with 1-octanol
-
-
r
methanol + NAD+
formaldehyde + NADH + H+
methyl 3-oxobutanoate + NADH + H+
methyl 3-hydroxybutanoate + NAD+
-
130% of the activity with phenyl trifluoromethyl ketone
-
-
?
methyl 4-bromo-3-oxobutanoate + NADH + H+
methyl 4-bromo-3-hydroxybutanoate + NAD+
-
164% of the activity with phenyl trifluoromethyl ketone
-
-
?
methyl acetoacetate + NADH + H+
methyl 3-hydroxybutanoate + NAD+
-
-
-
-
r
methyl benzoylformate + NADH
?
methyl benzoylformate + NADH + H+
methyl (R)-(-)-mandelate + NAD+
-
92% enantiomeric excess
-
-
?
methyl benzoylformate + NADH + H+
methyl (S)-mandelate + NAD+
-
6 h, 100% conversion, 17% enantiomeric excess
-
r
methyl glyoxal + NADH + H+
?
-
3.5fold higher activity compared to activity with N-benzyl-3-pyrrolidinone
-
-
?
methyl o-chlorobenzoylformate + NADH
?
about 55% of the activity compared to isatin
-
-
?
methyl o-chlorobenzoylformate + NADH + H+
methyl (R)-o-chloromandelate + NAD+
-
6 h, 99% conversion, 72% enantiomeric excess
-
r
methyl oxo(phenyl)acetate + NADH + H+
methyl hydroxy(phenyl)acetate + NAD+
-
57% of the activity with 2,2,2-trifluoroacetophenone
-
-
r
methyl pyruvate + NADH + H+
methyl 2-hydroxypropanoate + NAD+
-
18.9fold higher activity compared to activity with N-benzyl-3-pyrrolidinone
-
-
?
methyl pyruvate + NADH + H+
methyl lactate + NAD+
-
-
-
-
r
methylbenzoylformate + NADH + H+
?
-
13% of the activity with 2,2,2-trifluoroacetophenone
-
-
?
methylcrotonyl alcohol + NAD+
methylcrotonaldehyde + NADH
-
-
-
-
?
methylglyoxal + NADH
? + NADH
-
11.9% of the activity with acetaldehyde
-
-
?
N-benzyl-3-piperidone + NADH + H+
N-benzylpiperidin-3-ol + NAD+
-
2.3fold higher activity compared to activity with N-benzyl-3-pyrrolidinone
-
-
?
N-benzyl-3-pyrrolidinol + NAD+
1-benzylpyrrolidin-3-one + NADH + H+
-
33% of the activity with (S)-N-benzyl-3-pyrrolidinol
-
-
?
N-benzyl-3-pyrrolidinone + NADH + H+
(R)-N-benzyl-3-pyrrolidinol + NAD+
-
(R)-stereoselectivity of the reduction carried out with the heat-treated cells
-
-
r
N-benzyl-3-pyrrolidinone + NADH + H+
(S)-N-benzyl-3-pyrrolidinol + NAD+
N-benzyl-4-piperidone + NADH + H+
N-benzylpiperidin-4-ol + NAD+
-
10.7fold higher activity compared to activity with N-benzyl-3-pyrrolidinone
-
-
?
n-butanal + NADH + H+
n-butanol + NAD+
-
-
-
-
?
n-butanol + NAD+
butanal + NADH
-
low activity
-
-
?
n-butanol + NAD+
butylaldehyde + NADH + H+
n-butanol + NAD+
butyraldehyde + NADH
-
-
-
-
?
n-butanol + NAD+
butyraldehyde + NADH + H+
-
-
-
-
r
n-butanol + NAD+
n-butanal + NADH
n-butanol + NAD+
n-butanal + NADH + H+
-
-
-
-
?
n-butanol + NADH + H+
butyraldehyde + NAD+
-
-
-
-
r
n-butylaldehyde + NADH + H+
butanal + NAD+
-
activity is 1.8fold higher than with N-benzyl-3-pyrrolidinone
-
-
?
n-butyraldehyde + NADH + H+
n-butanol + NAD+
-
7.3fold higher activity compared to activity with N-benzyl-3-pyrrolidinone
-
-
?
n-decanol + NAD+
n-decanal + NADH
-
-
-
-
r
n-hexanol + NAD+
n-hexanal + NADH
-
-
-
-
r
n-hexylaldehyde + NADH + H+
hexanal + NAD+
-
activity is 1.6fold higher than with N-benzyl-3-pyrrolidinone
-
-
?
n-octylaldehyde + NADH + H+
n-octanol + NAD+
-
activity is 1.7fold higher than with N-benzyl-3-pyrrolidinone
-
-
?
n-pentanol + NAD+
pentanal + NADH + H+
-
-
-
-
?
n-pentylaldehyde + NADH + H+
n-pentanol + NAD+
-
-
-
-
r
n-propanol + NAD+
n-propanal + NADH
-
-
-
-
r
n-propanol + NAD+
propanal + NADH
n-propanol + NAD+
propanal + NADH + H+
n-propanol + NAD+
propionaldehyde + NADH
-
-
-
-
?
N-tert-butoxycarbonyl-3-pyrrolidinone + NADH + H+
(S)-N-tert-butoxycarbonyl-3-pyrrolidinol + NAD+
-
production of (S)-N-tert-butoxycarbonyl-3-pyrrolidinol with 99.6% enantiomeric excess
-
-
?
n-valeraldehyde + NADH + H+
n-pentanol + NAD+
-
activity is 2.8fold higher than with N-benzyl-3-pyrrolidinone
-
-
?
nonyl aldehyde + NADH + H+
1-nonanol + NAD+
25% activity compared to benzyl alcohol
-
-
?
nonylphenol polyethoxylate 9 + NAD+
? + NADH
7% activity compared to benzyl alcohol
-
-
?
octan-1-ol + NAD+
n-octanal + NADH
octan-2-ol + NAD+
octan-2-one + NADH
octanal + NAD+ + H2O
octanoic acid + NADH + H+
octanal + NADH + H+
octanol + NAD+
octanoic acid + 2 NADH + 2 H+
1-octanol + 2 NAD+ + H2o
-
-
-
r
octanol + NAD+
octanal + NADH
octanol + NAD+
octanal + NADH + H+
octyl aldehyde + NADH + H+
1-octanol + NAD+
29% activity compared to benzyl alcohol
-
-
?
octylphenol polyethoxylate 2 + NAD+
? + NADH
59% activity compared to benzyl alcohol
-
-
?
octylphenol polyethoxylate 8 + NAD+
? + NADH
7% activity compared to benzyl alcohol
-
-
?
oxalacetic acid + NADH + H+
?
-
14% of activity with N-benzyl-3-pyrrolidinone
-
-
?
oxaloacetate + NADH + H+
? + NAD+
-
-
-
-
r
p-chlorobenzaldehyde + NADH + H+
p-chlorobenzyl alcohol + NAD+
-
1.2fold higher activity compared to activity with N-benzyl-3-pyrrolidinone
-
-
?
p-methoxybenzyl alcohol + NAD+
p-methoxybenzaldehyde + NADH + H+
p-nitrobenzaldehyde + NADH
p-nitrobenzyl alcohol + NAD+
-
-
-
-
?
p-nitrobenzaldehyde + NADH + H+
p-nitrobenzyl alcohol + NAD+
-
substrate of isozyme ADH4
-
-
r
p-nitrobenzyloxycarbonyl-3-pyrrolidinone + NADH + H+
(S)-N-p-nitrobenzyloxycarbonyl-3-pyrrolidinol + NAD+
-
production with 90.7% enantiomeric excess
-
-
?
p-nitrophenyl octanoate + H2O
p-nitrophenol + octanoate
-
acid-assisted nucleophilic catalysis involving the ammonium ion of Lys and the thiolate of Cys in the acyl-oxygen cleavage
-
?
pentan-1-ol + NAD+
pentanal + NADH + H+
-
-
76% of the activity with butan-2-ol
-
?
pentan-2-ol + NAD+
2-pentanone + NADH
pentan-2-ol + NAD+
pentan-2-one + NADH + H+
pentan-3-ol + NAD+
3-pentanone + NADH
pentan-3-ol + NAD+
3-pentanone + NADH + H+
-
-
-
-
r
pentanal + NADH
1-pentanol + NAD+ + H+
-
132% of the activity with phenyl trifluoromethyl ketone, in the reverse reaction 5% of the activity with 2-propanol
-
-
?
pentanol + NAD+
1-pentanal + NADH + H+
about 10% of the activity with ethanol or 1-propanol
-
-
?
pentanol + NAD+
n-pentanal + NADH
pentanol + NAD+
pentanal + NADH + H+
pentanol + NAD+
pentanone + NADH
pentanol + NAD+
valeraldehyde + NADH + H+
-
240% activity compared to cyclohexanone
-
r
phenyl trifluoromethyl ketone + NADH + H+
(S)-1-phenyltrifluoroethanol + NAD+
-
-
more than 99% enantiomeric excess
-
?
phenylacetaldehyde + NADH
phenylethanol + NAD+
phenylalaninol + NAD+
? + NADH
-
-
-
-
?
phenylethanol + NAD+
phenylacetaldehyde + NADH
-
R-(+)-phenylethanol and S-(-)-phenylethanol
-
-
?
phenylethanol + NAD+
phenylacetaldehyde + NADH + H+
-
15% of activity with N-benzyl-3-pyrrolidinol
-
-
?
phenylglyoxylic acid + NADH + H+
hydroxy(phenyl)acetic acid + NAD+
-
enzyme covalently immobilized to magnetic Fe3O4 nanoparticles via glutaraldehyde retains 48.77% activity of its original activity
-
-
?
phytol + NAD+
phytenal + NADH + H+
-
-
-
-
?
polyoxyethylene 8 decyl ether + NAD+
? + NADH
12% activity compared to benzyl alcohol
-
-
?
primary or secondary alcohol + NAD+
aldehyde or ketone + NADH
prop-2-en-1-ol + NAD+
? + NADH
-
140.6% of the activity with ethanol
-
-
?
prop-2-en-1-ol+ NAD+
prop-2-enal + NADH + H+
-
-
85% of the activity with butan-2-ol
-
?
propan-1,2-diol + NAD+
? + NADH
propan-1-ol + NAD+
propanal + NADH + H+
propan-1-ol+ NAD+
propanal + NADH + H+
-
-
68% of the activity with butan-2-ol
-
r
propan-2-ol + NAD+
acetone + NADH
propan-2-ol + NAD+
acetone + NADH + H+
propan-2-ol+ NAD+
acetone + NADH + H+
-
-
79% of the activity with butan-2-ol
-
?
propanal + NADH + H+
propan-1-ol + NAD+
-
-
38% of the activity with butan-2-ol
-
r
propanol + NADH
propionaldehyde + NADH
propionaldehyde + NADH
propanol + NAD+
propionaldehyde + NADH + H+
1-propanol + NAD+
-
-
-
-
r
propionaldehyde + NADH + H+
?
-
activity is 1.1fold higher than with N-benzyl-3-pyrrolidinone
-
-
?
propiophenone + NADH + H+
1-phenylpropan-1-ol + NAD+
-
27% of activity with N-benzyl-3-pyrrolidinone
-
-
?
pyridine 2-aldehyde + NADH + H+
? + NAD+
-
-
-
-
r
pyridine 3-aldehyde + NADH + H+
? + NAD+
-
-
-
-
r
pyridine 4-aldehyde + NADH + H+
? + NAD+
-
-
-
-
r
pyridine-3-aldehyde + NADH + H+
pyridin-3-yl ethanol + NAD+
-
82% of activity with N-benzyl-3-pyrrolidinone
-
-
?
pyridine-4-aldehyde + NADH + H+
pyridin-4-yl methanol + NAD+
-
4.4fold higher activity compared to activity with N-benzyl-3-pyrrolidinone
-
-
?
pyruvaldehyde + NADH + H+
lactaldehyde + NAD+
81% of the activity with acetoin
-
-
?
R-(+)-1-phenylethanol + NAD+
1-phenylethanone + NADH
-
-
-
-
-
R-(+)-cis-verbenol + NAD+
? + NADH
-
-
-
-
?
R-(+)-trans-bicyclo(2.2.1)-heptanol + NAD+
R-(+)-trans-bicyclo(2.2.1)-heptanal + NADH
-
R-(+)-trans-bicyclo(2.2.1)-heptanol and S-(-)-trans-bicyclo(2.2.1)-heptanol
-
-
?
rac-3-methylcyclohexanone + NADH + H+
(1S,3S)-3-methylcyclohexanol + NAD+
-
-
-
-
?
retinal + NADH + H+
retinol + NAD+
-
substrate of isozyme ADH4
-
-
r
retinol + NAD+
retinal + NADH
-
-
-
-
?
S-(-)-1-phenylethanol + NAD+
1-phenylethanone + NADH
-
-
-
-
-
S-(-)-cis-bicyclo(2.2.1)-heptanol + NAD+
S-(-)-cis-bicyclo(2.2.1)-heptanal + NADH
-
-
-
-
?
secondary alcohol + NAD+
aldehyde + NADH
-
-
-
-
?
tert-butyl 3-oxobutanoate + NADH + H+
tert butyl 3-hydroxybutanoate + NAD+
-
568% of the activity with phenyl trifluoromethyl ketone
-
-
?
tert-butyl acetoacetate + NADH + H+
?
-
-
-
-
?
tetracosanol + NAD+
? + NADH + H+
-
immobilized HLAD shows about 55% reaction rate and free HLAD shows about 15% reaction rate with tetracosanol compared to ethanol at pH 8.8 and 30°C
-
-
?
tetrahydro-4H-pyran-4-one + NADH + H+
? + NAD+
10% activity compared to cyclohexanone
-
-
?
tetralin-1-ol + NAD+
3,4-dihydronaphthalen-1(2H)-one + NADH + H+
51% of the activity compared to isoborneol
-
-
?
trans-4-(N,N-dimethylamino)-cinnamaldehyde + NADH
trans-4-(N,N-dimethylamino)-cinnamyl alcohol + NAD+
-
-
-
-
?
trans-4-methylcyclohexanol + NAD+
4-methylcyclohexanone + NADH
-
-
-
-
?
trans-cinnamaldehyde + NADH + H+
cinnamyl alcohol + NAD+
trans-cinnamyl alcohol + NAD+
(2E)-3-phenylprop-2-enal + NADH + H+
-
activity is 3.9fold higher than with (S)-(-)-1-phenylethanol
-
-
?
Tris + NAD+
? + NADH
-
-
-
-
r
Triton X-100 + NAD+
? + NADH
7% activity compared to benzyl alcohol
-
-
?
Triton X-114 + NAD+
? + NADH
3% activity compared to benzyl alcohol
-
-
?
Triton X-165 + NAD+
? + NADH
24% activity compared to benzyl alcohol
-
-
?
Triton X-35 + NAD+
? + NADH
43% activity compared to benzyl alcohol
-
-
?
Triton X-405 + NAD+
? + NADH
19% activity compared to benzyl alcohol
-
-
?
Triton X-45 + NAD+
? + NADH
7% activity compared to benzyl alcohol
-
-
?
tryptophol + NAD+
? + NADH
-
-
-
-
?
valeraldehyde + NADH
1-pentanol + NAD+
vanillyl alcohol + NAD+
vanillin + NADH
-
-
-
-
?
additional information
?
-
(2E)-2-methylpent-2-enal + NADPH + H+

(2E)-2-methylpent-2-en-1-ol + NADP+
41.5% of the activity with (2E)-but-2-enal, yield 95% after 12 h
-
-
?
(2E)-2-methylpent-2-enal + NADPH + H+
(2E)-2-methylpent-2-en-1-ol + NADP+
41.5% of the activity with (2E)-but-2-enal, yield 95% after 12 h
-
-
?
(2E)-3,7-dimethylocta-2,6-dienal + NADPH + H+

(2E)-3,7-dimethylocta-2,6-dien-1-ol + NADP+
37.4% of the activity with (2E)-but-2-enal, yield 87% after 12 h
-
-
?
(2E)-3,7-dimethylocta-2,6-dienal + NADPH + H+
(2E)-3,7-dimethylocta-2,6-dien-1-ol + NADP+
37.4% of the activity with (2E)-but-2-enal, yield 87% after 12 h
-
-
?
(E)-hex-2-en-1-ol + NAD+

(E)-hex-2-en-1-one + NADH
-
43.2% of the activity with ethanol
-
-
?
(E)-hex-2-en-1-ol + NAD+
(E)-hex-2-en-1-one + NADH
-
-
-
-
?
(R)-2-butanol + NAD+

2-butanone + NADH + H+
-
55% of activity with N-benzyl-3-pyrrolidinol
-
-
?
(R)-2-butanol + NAD+
2-butanone + NADH + H+
-
55% of activity with N-benzyl-3-pyrrolidinol
-
-
?
(R)-2-butanol + NAD+
2-butanone + NADH + H+
-
50% of the activity with 2-propanol
-
-
ir
(R)-2-butanol + NAD+
2-butanone + NADH + H+
-
50% of the activity with 2-propanol
-
-
ir
(R)-2-butanol + NAD+
2-butanone + NADH + H+
-
-
-
?
(R)-2-butanol + NAD+
2-butanone + NADH + H+
-
-
-
?
(R)-2-butanol + NAD+
2-butanone + NADH + H+
-
-
-
-
?
(R)-2-butanol + NAD+
2-butanone + NADH + H+
-
-
-
-
r
(R)-2-heptanol + NAD+

2-heptanone + NADH + H+
-
2469% of the activity with 2-propanol
-
-
r
(R)-2-heptanol + NAD+
2-heptanone + NADH + H+
-
2469% of the activity with 2-propanol
-
-
r
(R)-2-hexanol + NAD+

2-hexanone + NADH + H+
-
1906% of the activity with 2-propanol
-
-
r
(R)-2-hexanol + NAD+
2-hexanone + NADH + H+
-
1906% of the activity with 2-propanol
-
-
r
(R)-2-octanol + NAD+

2-octanone + NADH + H+
-
-
-
-
-
(R)-2-octanol + NAD+
2-octanone + NADH + H+
-
2406% of the activity with 2-propanol
-
-
r
(R)-2-octanol + NAD+
2-octanone + NADH + H+
-
2406% of the activity with 2-propanol
-
-
r
(R)-2-pentanol + NAD+

2-pentanone + NADH + H+
-
-
-
-
-
(R)-2-pentanol + NAD+
2-pentanone + NADH + H+
-
75% of the activity with 2-propanol
-
-
r
(R)-2-pentanol + NAD+
2-pentanone + NADH + H+
-
-
-
-
r
(R)-2-phenylpropanol + NAD+

(R)-2-phenylpropanal + NADH + H+
-
63% of the activity with 2-phenylethanol
-
-
?
(R)-2-phenylpropanol + NAD+
(R)-2-phenylpropanal + NADH + H+
-
63% of the activity with 2-phenylethanol
-
-
?
(R,S)-2-methylbutan-1-ol + NAD+

(R,S)-2-methyl-butan-1-one + NADH + H+
-
-
-
?
(R,S)-2-methylbutan-1-ol + NAD+
(R,S)-2-methyl-butan-1-one + NADH + H+
-
-
-
?
(R,S)-2-methylbutan-1-ol + NAD+
(R,S)-2-methyl-butan-1-one + NADH + H+
-
-
-
-
?
(S)-1-indanol + NAD+

1-indanone + NADH + H+
-
-
-
r
(S)-1-indanol + NAD+
1-indanone + NADH + H+
-
3900% of the activity with (S)-1-phenylethanol
-
-
r
(S)-2-butanol + NAD+

2-butanone + NADH + H+
-
275% of the activity with 2-propanol
-
-
ir
(S)-2-butanol + NAD+
2-butanone + NADH + H+
-
-
-
?
(S)-2-butanol + NAD+
2-butanone + NADH + H+
-
-
-
?
(S)-2-butanol + NAD+
2-butanone + NADH + H+
-
-
-
-
?
(S)-2-butanol + NAD+
2-butanone + NADH + H+
-
-
-
-
r
(S)-2-methylbutan-1-ol + NAD+

(S)-2-methyl-butanal + NADH + H+
-
-
-
?
(S)-2-methylbutan-1-ol + NAD+
(S)-2-methyl-butanal + NADH + H+
-
-
-
-
?
(S)-2-methylbutan-1-ol + NAD+
(S)-2-methyl-butanal + NADH + H+
-
-
-
-
-
(S)-2-octanol + NAD+

2-octanone + NADH + H+
-
-
-
-
-
(S)-2-octanol + NAD+
2-octanone + NADH + H+
-
81% of the activity with 2-propanol
-
-
r
(S)-2-pentanol + NAD+

2-pentanone + NADH + H+
-
-
-
-
-
(S)-2-pentanol + NAD+
2-pentanone + NADH + H+
-
25% of the activity with 2-propanol
-
-
r
(S)-2-pentanol + NAD+
2-pentanone + NADH + H+
-
-
-
-
r
(S)-alpha-tetralol + NAD+

alpha-tetralone + NADH + H+
-
-
-
r
(S)-alpha-tetralol + NAD+
alpha-tetralone + NADH + H+
-
-
-
r
(S)-alpha-tetralol + NAD+
alpha-tetralone + NADH + H+
-
2700% of the activity with (S)-1-phenylethanol
-
-
r
1,2-butanediol + NAD+

?
-
35% of the activity with (S)-N-benzyl-3-pyrrolidinol
-
-
?
1,2-butanediol + NAD+
?
low activity
-
-
?
1,2-butanediol + NAD+
?
low activity
-
-
?
1,2-propanediol + NAD+

?
-
-
-
-
?
1,2-propanediol + NAD+
?
-
-
-
-
?
1,3-propanediol + 2 NAD+ + H2O

? + 2 NADH + 2 H+
about 45% of the activity with ethanol
-
-
r
1,3-propanediol + 2 NAD+ + H2O
? + 2 NADH + 2 H+
about 5% of the activity with 1-octanol
-
-
r
1,3-propanediol + 2 NAD+ + H2O
? + 2 NADH + 2 H+
about 45% of the activity with ethanol
-
-
r
1,3-propanediol + 2 NAD+ + H2O
? + 2 NADH + 2 H+
about 5% of the activity with 1-octanol
-
-
r
1,3-propanediol + NAD+

?
-
-
-
-
?
1,3-propanediol + NAD+
?
-
-
-
-
?
1-(4-fluorophenyl)ethanol + NAD+

1-(4-fluorophenyl)ethanone + NADH + H+
119% of the activity with 1-phenylethanol
-
-
?
1-(4-fluorophenyl)ethanol + NAD+
1-(4-fluorophenyl)ethanone + NADH + H+
-
45% of the activity with (S)-(-)-1-phenylethanol
-
-
?
1-butanol + 2 NAD+ + H2O

butanoic acid + 2 NADH + 2 H+
about 45% of the activity with ethanol
-
-
r
1-butanol + 2 NAD+ + H2O
butanoic acid + 2 NADH + 2 H+
about 50% of the activity with 1-octanol
-
-
r
1-butanol + 2 NAD+ + H2O
butanoic acid + 2 NADH + 2 H+
about 45% of the activity with ethanol
-
-
r
1-butanol + 2 NAD+ + H2O
butanoic acid + 2 NADH + 2 H+
about 50% of the activity with 1-octanol
-
-
r
1-butanol + NAD+

butanal + NADH
-
111% of the activity with 2-phenylethanol
-
-
?
1-butanol + NAD+
butanal + NADH
-
111% of the activity with 2-phenylethanol
-
-
?
1-butanol + NAD+
butanal + NADH
-
-
-
-
?
1-butanol + NAD+

butanal + NADH + H+
-
-
-
-
?
1-butanol + NAD+
butanal + NADH + H+
-
-
-
-
?
1-butanol + NAD+
butanal + NADH + H+
-
-
-
r
1-butanol + NAD+
butanal + NADH + H+
-
-
-
r
1-butanol + NAD+
butanal + NADH + H+
-
-
-
?
1-butanol + NAD+
butanal + NADH + H+
-
-
-
?
1-butanol + NAD+
butanal + NADH + H+
-
-
-
-
?
1-butanol + NAD+
butanal + NADH + H+
-
-
-
-
?
1-butanol + NAD+
butanal + NADH + H+
-
15.7% of the activity with ethanol
-
-
?
1-butanol + NAD+
butanal + NADH + H+
about 80% of activity with ethanol, ADH1
-
-
?
1-butanol + NAD+
butanal + NADH + H+
about 80% of activity with ethanol, ADH1
-
-
?
1-butanol + NAD+
butanal + NADH + H+
-
-
-
-
r
1-butanol + NAD+
butanal + NADH + H+
-
-
-
-
r
1-butanol + NAD+
butanal + NADH + H+
-
-
-
r
1-butanol + NAD+
butanal + NADH + H+
-
-
-
r
1-butanol + NAD+
butanal + NADH + H+
30% activity compared to benzyl alcohol
-
-
?
1-butanol + NAD+
butanal + NADH + H+
30% activity compared to benzyl alcohol
-
-
?
1-butanol + NAD+
butanal + NADH + H+
-
-
-
-
r
1-butanol + NAD+
butanal + NADH + H+
67.7% activity compared to ethanol
-
-
?
1-butanol + NAD+
butanal + NADH + H+
142% activity compared to cyclohexanol
-
-
r
1-butanol + NAD+
butanal + NADH + H+
142% activity compared to cyclohexanol
-
-
r
1-decanol + NAD+

decanal + NADH + H+
-
-
-
r
1-decanol + NAD+
decanal + NADH + H+
-
-
-
r
1-decanol + NAD+
decanal + NADH + H+
13% activity compared to benzyl alcohol
-
-
?
1-decanol + NAD+
decanal + NADH + H+
13% activity compared to benzyl alcohol
-
-
?
1-dodecanol + NAD+

dodecanal + NADH
-
68% of the activity with 2-phenylethanol
-
-
?
1-dodecanol + NAD+
dodecanal + NADH
-
68% of the activity with 2-phenylethanol
-
-
?
1-heptanol + NAD+

heptanal + NADH + H+
-
-
-
r
1-heptanol + NAD+
heptanal + NADH + H+
-
-
-
r
1-heptanol + NAD+
heptanal + NADH + H+
-
-
-
?
1-heptanol + NAD+
heptanal + NADH + H+
-
-
-
?
1-heptanol + NAD+
heptanal + NADH + H+
25% activity compared to benzyl alcohol
-
-
?
1-heptanol + NAD+
heptanal + NADH + H+
25% activity compared to benzyl alcohol
-
-
?
1-heptanol + NAD+
heptanal + NADH + H+
80% activity compared to cyclohexanol
-
-
r
1-heptanol + NAD+
heptanal + NADH + H+
80% activity compared to cyclohexanol
-
-
r
1-hexanol + 2 NAD+ + H2O

hexanoic acid + 2 NADH + 2 H+
about 55% of the activity with 1-octanol
-
-
r
1-hexanol + 2 NAD+ + H2O
hexanoic acid + 2 NADH + 2 H+
about 70% of the activity with ethanol
-
-
r
1-hexanol + 2 NAD+ + H2O
hexanoic acid + 2 NADH + 2 H+
about 70% of the activity with ethanol
-
-
r
1-hexanol + 2 NAD+ + H2O
hexanoic acid + 2 NADH + 2 H+
about 55% of the activity with 1-octanol
-
-
r
1-hexanol + NAD+

1-hexanal + NADH + H+
-
-
-
-
?
1-hexanol + NAD+
1-hexanal + NADH + H+
-
-
-
-
r
1-hexanol + NAD+

hexanal + NADH + H+
-
-
-
r
1-hexanol + NAD+
hexanal + NADH + H+
-
-
-
?
1-hexanol + NAD+
hexanal + NADH + H+
-
-
-
?
1-hexanol + NAD+
hexanal + NADH + H+
-
44% of the activity with 2-propanol, in the reverse reaction 1029% of the activity with phenyl trifluoromethyl ketone
-
-
r
1-hexanol + NAD+
hexanal + NADH + H+
about 90% of activity with ethanol, ADH1
-
-
?
1-hexanol + NAD+
hexanal + NADH + H+
about 90% of activity with ethanol, ADH1
-
-
?
1-hexanol + NAD+
hexanal + NADH + H+
61% activity compared to benzyl alcohol
-
-
?
1-hexanol + NAD+
hexanal + NADH + H+
61% activity compared to benzyl alcohol
-
-
?
1-hexanol + NAD+
hexanal + NADH + H+
-
-
-
?
1-hexanol + NAD+
hexanal + NADH + H+
15.3% activity compared to ethanol
-
-
?
1-hexanol + NAD+
hexanal + NADH + H+
109% activity compared to cyclohexanol
-
-
r
1-hexanol + NAD+
hexanal + NADH + H+
109% activity compared to cyclohexanol
-
-
r
1-indanol + NAD+

1-indanone + NADH + H+
26% of the activity compared to isoborneol
-
-
?
1-indanol + NAD+
1-indanone + NADH + H+
26% of the activity compared to isoborneol
-
-
?
1-indanol + NAD+
1-indanone + NADH + H+
-
1% activity compared to cyclohexanone
-
r
1-indanol + NAD+
1-indanone + NADH + H+
-
-
-
-
?
1-indanone + NADH + H+

(S)-1-indanol + NAD+
-
6 h, 50°C, 6% conversion, 25% enantiomeric excess
-
r
1-indanone + NADH + H+
(S)-1-indanol + NAD+
-
6 h, 50°C, 6% conversion, 25% enantiomeric excess
-
r
1-nonanol + NAD+

nonanal + NADH + H+
-
-
-
r
1-nonanol + NAD+
nonanal + NADH + H+
12% activity compared to benzyl alcohol
-
-
?
1-octanol + 2 NAD+ + H2O

octanoic acid + 2 NADH + 2 H+
best substrate
-
-
r
1-octanol + 2 NAD+ + H2O
octanoic acid + 2 NADH + 2 H+
about 30% of the activity with ethanol
-
-
r
1-octanol + 2 NAD+ + H2O
octanoic acid + 2 NADH + 2 H+
best substrate
-
-
r
1-octanol + NAD+

octanal + NADH
-
101% of the activity with 2-phenylethanol
-
-
?
1-octanol + NAD+
octanal + NADH
-
101% of the activity with 2-phenylethanol
-
-
?
1-octanol + NAD+

octanal + NADH + H+
-
-
-
r
1-octanol + NAD+
octanal + NADH + H+
-
-
-
?
1-octanol + NAD+
octanal + NADH + H+
-
-
-
?
1-octanol + NAD+
octanal + NADH + H+
substrate for isozyme ADH3
-
-
r
1-octanol + NAD+
octanal + NADH + H+
-
33% of the activity with 2-propanol, in the reverse reaction 435% of the activity with phenyl trifluoromethyl ketone
-
-
r
1-octanol + NAD+
octanal + NADH + H+
about 85% of activity with ethanol, ADH1
-
-
?
1-octanol + NAD+
octanal + NADH + H+
-
-
-
r
1-octanol + NAD+
octanal + NADH + H+
11% activity compared to benzyl alcohol
-
-
?
1-octanol + NAD+
octanal + NADH + H+
57% activity compared to cyclohexanol
-
-
r
1-pentanol + NAD+

1-pentanal + NADH + H+
-
-
-
-
?
1-pentanol + NAD+
1-pentanal + NADH + H+
-
-
-
-
r
1-pentanol + NAD+

pentanal + NADH + H+
-
-
-
r
1-pentanol + NAD+
pentanal + NADH + H+
-
-
-
?
1-pentanol + NAD+
pentanal + NADH + H+
about 85% of activity with ethanol, ADH1
-
-
?
1-pentanol + NAD+
pentanal + NADH + H+
-
-
-
r
1-pentanol + NAD+
pentanal + NADH + H+
152% activity compared to benzyl alcohol
-
-
?
1-pentanol + NAD+
pentanal + NADH + H+
19.1% activity compared to ethanol
-
-
?
1-pentanol + NAD+
pentanal + NADH + H+
121% activity compared to cyclohexanol
-
-
r
1-phenyl-1,2-propanedione + NADH + H+

?
1-phenyl-1,2-propanedione and ethyl 3-methyl-2-oxobutyrate are the best substrate in the reduction reaction
-
-
?
1-phenyl-1,2-propanedione + NADH + H+
?
1-phenyl-1,2-propanedione and ethyl 3-methyl-2-oxobutyrate are the best substrate in the reduction reaction
-
-
?
1-phenyl-1,2-propanedione + NADH + H+
?
-
activity is 1.46fold higher than with 2,2,2-trifluoroacetophenone
-
-
?
1-phenyl-2-butanone + NADH + H+

1-phenylbutan-2-ol + NAD+
-
1.5fold higher activity compared to activity with N-benzyl-3-pyrrolidinone
-
-
?
1-phenyl-2-butanone + NADH + H+
1-phenylbutan-2-ol + NAD+
-
1.5fold higher activity compared to activity with N-benzyl-3-pyrrolidinone
-
-
?
1-phenyl-2-butanone + NADH + H+
1-phenylbutan-2-ol + NAD+
-
12% of the activity with phenyl trifluoromethyl ketone
-
-
?
1-phenyl-2-propanol + NAD+

1-phenyl-2-propanone + NADH + H+
39% of the activity with 1-phenylethanol
-
-
?
1-phenyl-2-propanol + NAD+
1-phenyl-2-propanone + NADH + H+
3% activity compared to cyclohexanol
-
-
r
1-phenyl-2-propanol + NAD+
1-phenyl-2-propanone + NADH + H+
-
16% of the activity with (S)-(-)-1-phenylethanol
-
-
?
1-phenylethanol + NAD+

1-phenylethanone + NADH
-
46% of the activity with 2-phenylethanol
-
-
?
1-phenylethanol + NAD+
1-phenylethanone + NADH
-
-
-
?
1-phenylethanol + NAD+

1-phenylethanone + NADH + H+
-
-
-
?
1-phenylethanol + NAD+
1-phenylethanone + NADH + H+
3% activity compared to cyclohexanol
-
-
r
1-propanol + NAD+

propanal + NADH + H+
-
-
-
-
?
1-propanol + NAD+
propanal + NADH + H+
-
-
-
-
?
1-propanol + NAD+
propanal + NADH + H+
-
-
-
r
1-propanol + NAD+
propanal + NADH + H+
the enzyme shows a preference for short-chain alcohols ethanol and 1-propanol
-
-
?
1-propanol + NAD+
propanal + NADH + H+
the enzyme shows a preference for short-chain alcohols ethanol and 1-propanol
-
-
?
1-propanol + NAD+
propanal + NADH + H+
-
-
-
-
?
1-propanol + NAD+
propanal + NADH + H+
about 75% of activity with ethanol, ADH1
-
-
?
1-propanol + NAD+
propanal + NADH + H+
-
-
-
-
?
1-propanol + NAD+
propanal + NADH + H+
-
-
-
-
r
1-propanol + NAD+
propanal + NADH + H+
-
-
-
-
?
1-propanol + NAD+
propanal + NADH + H+
153% activity compared to cyclohexanol
-
-
r
1-propanol + NAD+

propanaldehyde + NADH + H+
-
-
-
-
r
1-propanol + NAD+
propanaldehyde + NADH + H+
-
-
-
r
11-cis-retinol + NAD+

11-cis-retinal + NADH
-
-
-
?
11-cis-retinol + NAD+
11-cis-retinal + NADH
-
-
-
-
?
11-cis-retinol + NAD+
11-cis-retinal + NADH
-
-
-
-
?
12-hydroxydodecanoate + NAD+

12-oxododecanoic acid + NADH
-
-
-
-
?
12-hydroxydodecanoate + NAD+
12-oxododecanoic acid + NADH
-
-
-
-
?
12-hydroxydodecanoate + NAD+
12-oxododecanoic acid + NADH
-
-
-
-
?
12-hydroxydodecanoate + NAD+
12-oxododecanoic acid + NADH
-
oxidized at pH 10, not oxidized at pH 7.5
-
-
-
12-hydroxydodecanoate + NAD+
12-oxododecanoic acid + NADH
-
-
-
-
?
13-cis-retinol + NAD+

13-cis-retinal + NADH
-
-
-
?
13-cis-retinol + NAD+
13-cis-retinal + NADH
-
no activity with isozyme ADH1
-
-
?
13-cis-retinol + NAD+
13-cis-retinal + NADH
-
no activity with isozyme ADH1
-
-
?
2,2,2-trifluoroacetophenone + NADH + H+

(R)-alpha-(trifluoromethyl)benzyl alcohol + NAD+
-
-
93% enantiomeric excess
-
r
2,2,2-trifluoroacetophenone + NADH + H+
(R)-alpha-(trifluoromethyl)benzyl alcohol + NAD+
-
93% enantiomeric excess
-
-
?
2,2,2-trifluoroacetophenone + NADH + H+

2,2,2-trifluoro-1-phenylethanol + NAD+
35% of the activity compared to 1-phenyl-1,2-propanedione
-
-
?
2,2,2-trifluoroacetophenone + NADH + H+
2,2,2-trifluoro-1-phenylethanol + NAD+
-
-
-
-
r
2,2-dichloroacetophenone + NADH + H+

2,2-dichloro-1-phenylethanol + NAD+
30% of the activity compared to 1-phenyl-1,2-propanedione
-
-
?
2,2-dichloroacetophenone + NADH + H+
2,2-dichloro-1-phenylethanol + NAD+
-
32% of the activity with 2,2,2-trifluoroacetophenone
-
-
?
2,3-butanediol + NAD+

acetoin + NADH + H+
-
-
-
?
2,3-butanediol + NAD+
acetoin + NADH + H+
272% of the activity with 1-phenylethanol
-
-
r
2,4-pentanediol + NAD+

?
-
21% of the activity with (S)-N-benzyl-3-pyrrolidinol
-
-
?
2,4-pentanediol + NAD+
?
4% activity compared to cyclohexanol
-
-
?
2-(3,5-dimethylphenyl)propanal + NADH + H+

(S)-2-(3,5-dimethylphenyl)propanol + NAD+
-
18 h, 99% yield, 90% enantiomeric excess
-
-
?
2-(3,5-dimethylphenyl)propanal + NADH + H+
(S)-2-(3,5-dimethylphenyl)propanol + NAD+
18 h, 99% yield, 90% enantiomeric excess
-
-
?
2-(3-benzoylphenyl)propanal + NADH + H+

(S)-2-(3-benzoylphenyl)propanol + NAD+
-
18 h, 85% yield, 95% enantiomeric excess
-
-
?
2-(3-benzoylphenyl)propanal + NADH + H+
(S)-2-(3-benzoylphenyl)propanol + NAD+
18 h, 85% yield, 95% enantiomeric excess
-
-
?
2-(3-fluorobiphenyl-4-yl)propanal + NADH + H+

(S)-2-(3-(fluoro)biphenyl-4-yl)propanol + NAD+
-
18 h, 77% yield, 97% enantiomeric excess
-
-
?
2-(3-fluorobiphenyl-4-yl)propanal + NADH + H+
(S)-2-(3-(fluoro)biphenyl-4-yl)propanol + NAD+
18 h, 77% yield, 97% enantiomeric excess
-
-
?
2-(3-phenoxyphenyl)propanal + NADH + H+

(S)-2-((3-phenoxy)phenyl)propanol + NAD+
-
18 h, 85% yield, 95% enantiomeric excess
-
-
?
2-(3-phenoxyphenyl)propanal + NADH + H+
(S)-2-((3-phenoxy)phenyl)propanol + NAD+
18 h, 85% yield, 95% enantiomeric excess
-
-
?
2-(4-isobutylphenyl)propanal + NADH + H+

(S)-2-(4-isobutylphenyl)propanol + NAD+
-
18 h, 92% yield, 99% enantiomeric excess
-
-
?
2-(4-isobutylphenyl)propanal + NADH + H+
(S)-2-(4-isobutylphenyl)propanol + NAD+
18 h, 92% yield, 99% enantiomeric excess
-
-
?
2-butanol + NAD+

2-butanone + NADH + H+
-
-
-
-
?
2-butanol + NAD+
2-butanone + NADH + H+
-
-
-
-
?
2-butanol + NAD+
2-butanone + NADH + H+
-
-
-
-
?
2-butanol + NAD+
2-butanone + NADH + H+
-
activity is 3.7fold higher than with (S)-N-benzyl-3-pyrrolidinol
-
-
r
2-butanol + NAD+
2-butanone + NADH + H+
weak activity
-
-
r
2-butanol + NAD+
2-butanone + NADH + H+
about 25% of activity with ethanol, ADH1
-
-
?
2-butanol + NAD+
2-butanone + NADH + H+
39% activity compared to cyclohexanol
4% activity compared to cyclohexanone
-
r
2-butanone + NADH + H+

2-butanol + NAD+
-
-
-
-
r
2-butanone + NADH + H+
2-butanol + NAD+
-
40% of the activity with N-benzyl-3-pyrrolidinone
-
-
r
2-butanone + NADPH + H+

butan-2-ol + NADP+
12.1% of the activity with 2-hydroxyacetophenone and NADPH
-
-
?
2-butanone + NADPH + H+
butan-2-ol + NADP+
12.1% of the activity with 2-hydroxyacetophenone and NADPH
-
-
?
2-butene-1-ol + NAD+

? + NADH
-
-
-
-
?
2-butene-1-ol + NAD+
? + NADH
-
-
-
-
?
2-heptanol + NAD+

2-heptanone + NADH + H+
weak activity
-
-
r
2-heptanol + NAD+
2-heptanone + NADH + H+
63% activity compared to cyclohexanol
5% activity compared to cyclohexanone
-
r
2-heptanone + NADPH + H+

heptan-2-ol + NADP+
6.8% of the activity with 2-hydroxyacetophenone and NADPH
-
-
?
2-heptanone + NADPH + H+
heptan-2-ol + NADP+
6.8% of the activity with 2-hydroxyacetophenone and NADPH
-
-
?
2-hexanol + NAD+

2-hexanone + NADH + H+
-
activity is 11.2fold higher than with (S)-N-benzyl-3-pyrrolidinol
-
-
?
2-hexanol + NAD+
2-hexanone + NADH + H+
weak activity
-
-
r
2-hexanol + NAD+
2-hexanone + NADH + H+
64% activity compared to cyclohexanol
4% activity compared to cyclohexanone
-
r
2-hexanone + NADPH + H+

hexan-2-ol + NADP+
5.9% of the activity with 2-hydroxyacetophenone and NADPH
-
-
?
2-hexanone + NADPH + H+
hexan-2-ol + NADP+
5.9% of the activity with 2-hydroxyacetophenone and NADPH
-
-
?
2-hydroxyacetophenone + NADH + H+

(S)-1-phenyl-1,2-ethanediol + NAD+
2.4% of the activity with NADPH
-
-
?
2-hydroxyacetophenone + NADH + H+
(S)-1-phenyl-1,2-ethanediol + NAD+
2.4% of the activity with NADPH
-
-
?
2-mercaptoethanol + NAD+

?
-
-
-
-
?
2-mercaptoethanol + NAD+
?
11% activity compared to cyclohexanol
-
-
?
2-methylpropan-1-ol + NAD+

2-methyl-propan-1-one + NADH
-
-
-
-
?
2-methylpropan-1-ol + NAD+
2-methyl-propan-1-one + NADH
Sporotrichum pulverulentum
-
-
-
-
?
2-methylpropan-1-ol + NAD+
2-methyl-propan-1-one + NADH
-
-
-
-
-
2-methylpropan-1-ol + NAD+
2-methyl-propan-1-one + NADH
-
-
-
-
-
2-methylpropan-1-ol + NAD+
2-methyl-propan-1-one + NADH
-
-
-
-
?
2-methylpropan-1-ol + NAD+
2-methyl-propan-1-one + NADH
-
weak activity
-
-
?
2-methylpropan-1-ol + NAD+

2-methyl-propan-1-one + NADH + H+
-
-
-
?
2-methylpropan-1-ol + NAD+
2-methyl-propan-1-one + NADH + H+
-
-
-
-
?
2-octanol + NAD+

2-octanone + NADH + H+
weak activity
-
-
r
2-octanol + NAD+
2-octanone + NADH + H+
low activity
-
-
r
2-octanol + NAD+
2-octanone + NADH + H+
43% activity compared to cyclohexanol
4% activity compared to cyclohexanone
-
r
2-oxo-3-methylpentane + NADH

3-methylpentan-2-ol + NAD+
-
-
-
-
?
2-oxo-3-methylpentane + NADH
3-methylpentan-2-ol + NAD+
-
-
-
-
?
2-oxo-3-phenylpropane + NADH

2-hydroxy-3-phenylpropane + NAD+
-
-
-
-
?
2-oxo-3-phenylpropane + NADH
2-hydroxy-3-phenylpropane + NAD+
-
-
-
-
?
2-pentanol + NAD+

2-pentanone + NADH + H+
-
-
-
-
?
2-pentanol + NAD+
2-pentanone + NADH + H+
-
45% of activity with N-benzyl-3-pyrrolidinol
-
-
?
2-pentanol + NAD+
2-pentanone + NADH + H+
-
45% of activity with N-benzyl-3-pyrrolidinol
-
-
?
2-pentanol + NAD+
2-pentanone + NADH + H+
-
activity is 9fold higher than with (S)-N-benzyl-3-pyrrolidinol
-
-
?
2-pentanol + NAD+
2-pentanone + NADH + H+
weak activity
-
-
r
2-pentanol + NAD+
2-pentanone + NADH + H+
low activity
-
-
r
2-pentanol + NAD+
2-pentanone + NADH + H+
5.7% activity compared to ethanol
-
-
?
2-pentanone + NADH + H+

2-pentanol + NAD+
-
-
-
-
r
2-pentanone + NADH + H+
2-pentanol + NAD+
6% activity compared to cyclohexanol
-
-
r
2-propanol + NAD(P)+

acetone + NAD(P)H
-
-
-
-
?
2-propanol + NAD(P)+
acetone + NAD(P)H
-
-
-
-
?
2-propanol + NAD+

2-propanone + NADH
-
54% of the activity with 2-phenylethanol
-
-
?
2-propanol + NAD+
2-propanone + NADH
-
-
-
-
?
2-propanol + NAD+
2-propanone + NADH
-
-
-
?
2-propanol + NAD+

2-propanone + NADH + H+
-
activity is 3.1fold higher than with (S)-N-benzyl-3-pyrrolidinol
-
-
?
2-propanol + NAD+
2-propanone + NADH + H+
-
-
-
?
2-propanol + NAD+
2-propanone + NADH + H+
-
-
-
-
?
2-propanol + NAD+
2-propanone + NADH + H+
-
-
-
r
2-propanol + NAD+
2-propanone + NADH + H+
-
-
-
-
?
2-propanol + NAD+
2-propanone + NADH + H+
-
-
-
-
?
2-propanol + NAD+
2-propanone + NADH + H+
73.5% activity compared to ethanol
-
-
?
2-propanol + NAD+
2-propanone + NADH + H+
6% activity compared to cyclohexanol
-
-
r
2-propanol + NAD+

acetone + NADH + H+
-
-
-
-
?
2-propanol + NAD+
acetone + NADH + H+
-
-
-
-
?
2-propanol + NAD+
acetone + NADH + H+
-
-
-
r
2-propanol + NAD+
acetone + NADH + H+
-
-
-
r
2-propanol + NAD+
acetone + NADH + H+
-
-
-
-
ir
2-propanol + NAD+
acetone + NADH + H+
about 50% of activity with ethanol, ADH1
-
-
?
2-propanol + NAD+
acetone + NADH + H+
about 50% of activity with ethanol, ADH1
-
-
?
2-propanol + NAD+
acetone + NADH + H+
-
-
-
?
2-propanol + NAD+
acetone + NADH + H+
-
-
-
?
2-propanol + NAD+
acetone + NADH + H+
-
-
-
-
?
2-propanol + NAD+
acetone + NADH + H+
-
-
-
-
?
3-methoxybenzyl alcohol + NAD+

3-methoxybenzaldehyde + NADH + H+
-
-
-
-
?
3-methoxybenzyl alcohol + NAD+
3-methoxybenzaldehyde + NADH + H+
-
13% of the activity with (S)-(-)-1-phenylethanol
-
-
?
3-methylbutan-1-ol + NAD+

3-methyl-butan-1-one + NADH + H+
-
-
-
?
3-methylbutan-1-ol + NAD+
3-methyl-butan-1-one + NADH + H+
-
-
-
-
?
3-methylbutanal + NAD+

? + NADH + H+
about 10% of the activity with ethanol or 1-propanol
-
-
?
3-methylbutanal + NAD+
? + NADH + H+
about 10% of the activity with ethanol or 1-propanol
-
-
?
3-methylbutanol + NAD+

? + NADH
-
-
-
-
?
3-methylbutanol + NAD+
? + NADH
-
-
-
-
?
3-methylcyclohexanone + NADH

3-methylcyclohexanol + NAD+
about 10% of the activity compared to isatin
-
-
?
3-methylcyclohexanone + NADH
3-methylcyclohexanol + NAD+
about 10% of the activity compared to isatin
-
-
?
3-methylcyclohexanone + NADH
3-methylcyclohexanol + NAD+
-
-
-
-
r
3-nitrobenzaldehyde + NADH + H+

3-nitrobenzyl alcohol + NAD+
-
-
-
-
r
3-nitrobenzaldehyde + NADH + H+
3-nitrobenzyl alcohol + NAD+
-
class IV isozyme, reductive activity
-
-
?
3-oxo-5beta-androstan-17beta-ol + NADH

3beta,17beta-dihydroxy-5beta-androstane + NAD+
-
-
-
-
?
3-oxo-5beta-androstan-17beta-ol + NADH
3beta,17beta-dihydroxy-5beta-androstane + NAD+
-
-
-
-
?
3-pentanol + NAD+

3-pentanone + NADH + H+
-
-
-
-
r
3-pentanol + NAD+
3-pentanone + NADH + H+
2% activity compared to cyclohexanol
2% activity compared to cyclohexanone
-
r
3-phenyl-1-propanol + NAD+

3-phenyl-1-propanone + NADH
-
-
-
-
-
3-phenyl-1-propanol + NAD+
3-phenyl-1-propanone + NADH
-
-
-
-
?
3beta-hydroxy-5beta-androstan-17-one + NAD+

5beta-androstan-3,17-dione + NADH
-
-
-
-
?
3beta-hydroxy-5beta-androstan-17-one + NAD+
5beta-androstan-3,17-dione + NADH
-
catalyzed by isoenzyme BB-ADH, no activity with isoenzyme AA-ADH and TT-ADH
-
-
?
3beta-hydroxy-5beta-androstan-17-one + NAD+
5beta-androstan-3,17-dione + NADH
-
-
-
-
-
4-chloroacetophenone + NADH

1-(4-chlorophenyl)ethanol + NAD+
-
-
-
-
r
4-chloroacetophenone + NADH
1-(4-chlorophenyl)ethanol + NAD+
-
-
-
-
r
4-chloroacetophenone + NADH
1-(4-chlorophenyl)ethanol + NAD+
-
-
-
-
r
4-methoxybenzyl alcohol + NAD+

4-methoxybenzaldehyde + NADH + H+
-
-
-
-
?
4-methoxybenzyl alcohol + NAD+
4-methoxybenzaldehyde + NADH + H+
-
-
-
-
r
4-methoxybenzyl alcohol + NAD+
4-methoxybenzaldehyde + NADH + H+
-
99% of the activity with (S)-(-)-1-phenylethanol
-
-
?
4-methoxybenzyl alcohol + NAD+
4-methoxybenzaldehyde + NADH + H+
-
99% of the activity with (S)-1-phenylethanol
-
-
r
4-methyl-1-pentanol + NAD+

4-methyl-1-pentanal + NADH
-
-
-
-
-
4-methyl-1-pentanol + NAD+
4-methyl-1-pentanal + NADH
-
-
-
-
?
4-nitrobenzaldehyde + NADH + H+

4-nitrobenzyl alcohol + NAD+
-
-
-
-
r
4-nitrobenzaldehyde + NADH + H+
4-nitrobenzyl alcohol + NAD+
-
-
-
-
r
4-nitrobenzaldehyde + NADH + H+
4-nitrobenzyl alcohol + NAD+
-
-
-
-
?
5alpha-androstan-17beta-ol-3-one + NADH + H+

3beta,17beta-dihydroxy-5alpha-androstan + NAD+
-
-
-
-
-
5alpha-androstan-17beta-ol-3-one + NADH + H+
3beta,17beta-dihydroxy-5alpha-androstan + NAD+
-
-
-
-
?
5beta-androstan-17beta-ol-3-one + NAD+

5beta-androstan-3,17-dione + NADH
-
-
-
-
?
5beta-androstan-17beta-ol-3-one + NAD+
5beta-androstan-3,17-dione + NADH
-
low activity
-
-
?
5beta-androstan-3beta-ol-17-one + NAD+

5beta-androstan-3,17-dione + NADH
-
-
-
-
?
5beta-androstan-3beta-ol-17-one + NAD+
5beta-androstan-3,17-dione + NADH
-
-
-
-
?
7-cis-retinol + NAD+

7-cis-retinal + NADH
-
-
-
-
?
7-cis-retinol + NAD+
7-cis-retinal + NADH
-
-
-
-
?
9-cis-retinol + NAD+

9-cis-retinal + NADH
-
-
-
?
9-cis-retinol + NAD+
9-cis-retinal + NADH
-
-
-
-
?
9-cis-retinol + NAD+
9-cis-retinal + NADH
-
-
-
-
?
acetaldehyde + NADH + H+

ethanol + NAD+
-
-
-
-
r
acetaldehyde + NADH + H+
ethanol + NAD+
-
-
-
-
r
acetaldehyde + NADH + H+
ethanol + NAD+
-
-
-
-
r
acetaldehyde + NADH + H+
ethanol + NAD+
-
-
-
-
acetaldehyde + NADH + H+
ethanol + NAD+
-
-
-
-
acetaldehyde + NADH + H+
ethanol + NAD+
-
-
-
-
r
acetaldehyde + NADH + H+
ethanol + NAD+
-
the reduction of acetaldehyde is 4.9fold faster than the oxidation of ethanol
-
-
r
acetaldehyde + NADH + H+
ethanol + NAD+
-
-
-
r
acetaldehyde + NADH + H+
ethanol + NAD+
-
-
-
-
?
acetaldehyde + NADH + H+
ethanol + NAD+
-
-
-
-
?
acetaldehyde + NADH + H+
ethanol + NAD+
-
-
-
r
acetaldehyde + NADH + H+
ethanol + NAD+
-
-
-
r
acetaldehyde + NADH + H+
ethanol + NAD+
-
-
-
r
acetaldehyde + NADH + H+
ethanol + NAD+
-
-
-
-
r
acetaldehyde + NADH + H+
ethanol + NAD+
-
-
100% of the activity with butan-2-ol
-
r
acetaldehyde + NADH + H+
ethanol + NAD+
-
-
-
r
acetaldehyde + NADH + H+
ethanol + NAD+
-
-
-
r
acetaldehyde + NADH + H+
ethanol + NAD+
-
-
-
r
acetaldehyde + NADH + H+
ethanol + NAD+
-
-
-
r
acetaldehyde + NADH + H+
ethanol + NAD+
-
-
-
r
acetaldehyde + NADH + H+
ethanol + NAD+
-
-
-
-
?
acetaldehyde + NADH + H+
ethanol + NAD+
-
reduced by isoenzyme A2 and C2, no activity with isoenzyme B2
-
-
-
acetaldehyde + NADH + H+
ethanol + NAD+
-
-
-
-
r
acetaldehyde + NADH + H+
ethanol + NAD+
-
-
-
-
r
acetaldehyde + NADH + H+
ethanol + NAD+
-
-
-
r
acetaldehyde + NADH + H+
ethanol + NAD+
best substrate
-
-
r
acetaldehyde + NADH + H+
ethanol + NAD+
-
-
-
r
acetaldehyde + NADH + H+
ethanol + NAD+
best substrate
-
-
r
acetaldehyde + NADH + H+
ethanol + NAD+
-
-
-
r
acetaldehyde + NADH + H+
ethanol + NAD+
-
-
-
-
-
acetaldehyde + NADH + H+
ethanol + NAD+
-
-
-
-
r
acetaldehyde + NADH + H+
ethanol + NAD+
-
-
-
r
acetaldehyde + NADH + H+
ethanol + NAD+
-
-
-
-
r
acetaldehyde + NADH + H+
ethanol + NAD+
-
-
-
r
acetaldehyde + NADH + H+
ethanol + NAD+
-
-
-
-
r
acetaldehyde + NADH + H+
ethanol + NAD+
-
-
-
r
acetaldehyde + NADH + H+
ethanol + NAD+
-
cells with an extra copy of ADH1 display chronological life-span extension. Antioxidant enzymes are induced in 2xADH1 cells. Strains carrying an extra ADH1 copy show extended replicative life span and increased Sir2p activity
-
-
?
acetaldehyde + NADH + H+
ethanol + NAD+
mutant enzyme S109P/L116S/Y294C
-
-
?
acetaldehyde + NADH + H+
ethanol + NAD+
Sporotrichum pulverulentum
-
-
-
r
acetaldehyde + NADH + H+
ethanol + NAD+
-
-
-
?
acetaldehyde + NADH + H+
ethanol + NAD+
-
-
-
?
acetaldehyde + NADH + H+
ethanol + NAD+
-
reduced at the highest rate of all aldehydes tested
-
-
acetaldehyde + NADH + H+
ethanol + NAD+
-
-
-
-
r
acetaldehyde + NADH + H+

ethanol + NAD+ + H+
-
-
-
r
acetaldehyde + NADH + H+
ethanol + NAD+ + H+
-
-
-
?
acetaldehyde + NADH + H+
ethanol + NAD+ + H+
-
-
-
?
acetaldehyde + NADPH + H+

ethanol + NADP+
-
16% of the activity with NADH
-
-
r
acetaldehyde + NADPH + H+
ethanol + NADP+
-
-
-
r
acetaldehyde + NADPH + H+
ethanol + NADP+
-
NADPH is not a cofactor for wild-type, but for mutant P704L/H734R
-
-
r
acetaldehyde + NADPH + H+
ethanol + NADP+
-
-
-
r
acetaldehyde + NADPH + H+
ethanol + NADP+
-
NADPH is not a cofactor for wild-type, but for mutant P704L/H734R
-
-
r
acetaldehyde + NADPH + H+
ethanol + NADP+
-
-
-
?
acetaldehyde + NADPH + H+
ethanol + NADP+
-
-
-
?
acetone + NADH

isopropanol + NAD+
-
-
-
r
acetone + NADH
isopropanol + NAD+
-
-
-
r
acetophenone + NADH + H+

1-phenylethanol + NAD+
-
-
-
-
r
acetophenone + NADH + H+
1-phenylethanol + NAD+
-
-
-
-
r
acetophenone + NADH + H+
1-phenylethanol + NAD+
-
1.8fold higher activity compared to activity with N-benzyl-3-pyrrolidinone
-
-
?
acetophenone + NADH + H+
1-phenylethanol + NAD+
-
40% of the activity with N-benzyl-3-pyrrolidinone
-
-
?
acetophenone + NADH + H+
1-phenylethanol + NAD+
-
-
-
-
r
acetophenone + NADH + H+
1-phenylethanol + NAD+
-
-
-
-
r
acetophenone + NADH + H+
1-phenylethanol + NAD+
-
-
-
-
r
acetophenone + NADH + H+
1-phenylethanol + NAD+
-
-
-
?
acetophenone + NADH + H+
1-phenylethanol + NAD+
-
-
-
?
acetophenone + NADPH + H+

1-phenylethanol + NADP+
5.1% of the activity with 2-hydroxyacetophenone and NADPH
-
-
?
acetophenone + NADPH + H+
1-phenylethanol + NADP+
5.1% of the activity with 2-hydroxyacetophenone and NADPH
-
-
?
alcohol + NAD+

aldehyde or ketone + NADH
-
medium chain sec-alcohols or (omega-1)-ketones, no activity with primary alcohols or aldehydes
-
-
r
alcohol + NAD+
aldehyde or ketone + NADH
-
medium chain sec-alcohols or (omega-1)-ketones, no activity with primary alcohols or aldehydes
-
-
r
all-trans-retinol + NAD+

all-trans-retinal + NADH
-
-
-
?
all-trans-retinol + NAD+
all-trans-retinal + NADH
-
-
-
-
?
all-trans-retinol + NAD+
all-trans-retinal + NADH
-
-
-
-
r
all-trans-retinol + NAD+
all-trans-retinal + NADH
-
ADH4 might be involved in biosynthesis of retinoic acid
-
-
r
all-trans-retinol + NAD+
all-trans-retinal + NADH
-
-
-
-
?
all-trans-retinol + NAD+
all-trans-retinal + NADH
-
-
-
-
?
allyl alcohol + NAD+

acrolein + NADH
-
-
-
-
?
allyl alcohol + NAD+
acrolein + NADH
-
-
product is toxic in mouse hepatocytes due to cell protein carbonylation following exposure to crotyl alcohol
-
?
allyl alcohol + NAD+
acrolein + NADH
-
-
-
-
r
allyl alcohol + NAD+

prop-2-en-1-al + NADH
-
-
-
-
?
allyl alcohol + NAD+
prop-2-en-1-al + NADH
-
-
-
-
?
allyl alcohol + NAD+
prop-2-en-1-al + NADH
-
no activity
-
-
-
allyl alcohol + NAD+
prop-2-en-1-al + NADH
-
-
-
-
?
allyl alcohol + NAD+
prop-2-en-1-al + NADH
-
-
-
-
?
allyl alcohol + NAD+
prop-2-en-1-al + NADH
-
-
-
-
?
alpha-tetralone + NADH + H+

(S)-alpha-tetralol + NAD+
-
-
99% enantiomeric excess
-
r
alpha-tetralone + NADH + H+
(S)-alpha-tetralol + NAD+
-
more than 99% enantiomeric excess
-
-
?
benzaldehyde + NADH

benzyl alcohol + NAD+
-
-
-
-
?
benzaldehyde + NADH
benzyl alcohol + NAD+
-
-
-
-
?
benzaldehyde + NADH
benzyl alcohol + NAD+
-
reduced by isoenzyme A2 and C2, no activity with isoenzyme B2
-
-
r
benzaldehyde + NADH
benzyl alcohol + NAD+
-
-
-
?
benzaldehyde + NADH + H+

benzyl alcohol + NAD+
-
-
-
-
r
benzaldehyde + NADH + H+
benzyl alcohol + NAD+
-
-
-
-
r
benzaldehyde + NADH + H+
benzyl alcohol + NAD+
-
1.2% of the activity with acetaldehyde
-
-
?
benzaldehyde + NADH + H+
benzyl alcohol + NAD+
-
21% of activity with N-benzyl-3-pyrrolidinone
-
-
?
benzaldehyde + NADH + H+
benzyl alcohol + NAD+
-
74% of the activity with N-benzyl-3-pyrrolidinone
-
-
?
benzaldehyde + NADH + H+
benzyl alcohol + NAD+
-
-
93% of the activity with butan-2-ol
-
r
benzaldehyde + NADH + H+
benzyl alcohol + NAD+
3% activity compared to benzyl alcohol
-
-
r
benzaldehyde + NADH + H+
benzyl alcohol + NAD+
-
-
-
-
?
benzaldehyde + NADH + H+
benzyl alcohol + NAD+
-
-
-
-
?
benzaldehyde + NADH + H+
benzyl alcohol + NAD+
-
14% of the activity with 2,2,2-trifluoroacetophenone
-
-
?
benzaldehyde + NADPH + H+

benzyl alcohol + NADP+
107% of the activity with (2E)-but-2-enal, yield 99% after 12 h
-
-
r
benzaldehyde + NADPH + H+
benzyl alcohol + NADP+
107% of the activity with (2E)-but-2-enal, yield 99% after 12 h
-
-
r
benzyl alcohol + NAD+

benzaldehyde + NADH
-
-
-
-
?
benzyl alcohol + NAD+
benzaldehyde + NADH
-
-
-
-
?
benzyl alcohol + NAD+
benzaldehyde + NADH
-
-
-
-
?
benzyl alcohol + NAD+
benzaldehyde + NADH
-
-
-
-
?
benzyl alcohol + NAD+
benzaldehyde + NADH
-
-
-
-
?
benzyl alcohol + NAD+
benzaldehyde + NADH
-
-
-
r
benzyl alcohol + NAD+
benzaldehyde + NADH
-
oxidized by isoenzyme A2 and C2 no activity with isoenzyme B2
-
-
?
benzyl alcohol + NAD+
benzaldehyde + NADH
-
oxidation with isoenzyme ADH-1 and ADH-3, no activity with isoenzyme ADH-2
-
-
?
benzyl alcohol + NAD+
benzaldehyde + NADH
-
-
-
-
?
benzyl alcohol + NAD+
benzaldehyde + NADH
-
no activity
-
-
-
benzyl alcohol + NAD+

benzaldehyde + NADH + H+
-
i.e. phenylmethanol
-
-
?
benzyl alcohol + NAD+
benzaldehyde + NADH + H+
-
199% of the activity with 2-phenylethanol
-
-
?
benzyl alcohol + NAD+
benzaldehyde + NADH + H+
-
-
-
-
?
benzyl alcohol + NAD+
benzaldehyde + NADH + H+
-
-
-
-
?
benzyl alcohol + NAD+
benzaldehyde + NADH + H+
-
-
33% of the activity with butan-2-ol
-
r
benzyl alcohol + NAD+
benzaldehyde + NADH + H+
-
-
-
-
?
benzyl alcohol + NAD+
benzaldehyde + NADH + H+
-
-
-
-
?
benzyl alcohol + NAD+
benzaldehyde + NADH + H+
-
-
-
?
benzyl alcohol + NAD+
benzaldehyde + NADH + H+
-
-
-
?
benzyl alcohol + NAD+
benzaldehyde + NADH + H+
100% activity
-
-
r
benzyl alcohol + NAD+
benzaldehyde + NADH + H+
-
-
-
r
benzyl alcohol + NAD+
benzaldehyde + NADH + H+
-
-
-
r
benzyl alcohol + NAD+
benzaldehyde + NADH + H+
-
-
-
-
?
benzyl alcohol + NAD+
benzaldehyde + NADH + H+
-
-
-
-
r
benzyl alcohol + NAD+
benzaldehyde + NADH + H+
-
-
-
-
?
benzyl alcohol + NAD+
benzaldehyde + NADH + H+
-
-
-
-
r
benzyl alcohol + NAD+
benzaldehyde + NADH + H+
-
-
-
-
r
benzyl alcohol + NAD+
benzaldehyde + NADH + H+
-
-
-
?
benzyl alcohol + NAD+
benzaldehyde + NADH + H+
-
-
-
?
benzyl alcohol + NAD+
benzaldehyde + NADH + H+
-
-
-
-
?
benzyl alcohol + NAD+
benzaldehyde + NADH + H+
-
178% activity compared to cyclohexanone
-
r
benzyl alcohol + NAD+
benzaldehyde + NADH + H+
47% activity compared to cyclohexanol
154% activity compared to cyclohexanone
-
r
benzyl alcohol + NADP+

benzaldehyde + NADPH + H+
-
-
-
r
benzyl alcohol + NADP+
benzaldehyde + NADPH + H+
-
-
-
r
benzylacetone + NADH + H+

4-phenylbutan-2-ol + NAD+
-
-
-
-
r
benzylacetone + NADH + H+
4-phenylbutan-2-ol + NAD+
-
activity is 1.3fold higher than with N-benzyl-3-pyrrolidinone
-
-
?
benzylalcohol + NAD+

benzaldehyde + NADH + H+
weak activity
-
-
r
benzylalcohol + NAD+
benzaldehyde + NADH + H+
-
-
-
-
r
benzylalcohol + NAD+
benzaldehyde + NADH + H+
-
-
-
?
benzylalcohol + NAD+
benzaldehyde + NADH + H+
-
-
-
?
butan-2-ol + NAD+

butan-2-one + NADH
-
-
-
-
?
butan-2-ol + NAD+
butan-2-one + NADH
-
-
-
-
?
butan-2-ol + NAD+
butan-2-one + NADH
-
-
-
-
?
butan-2-ol + NAD+
butan-2-one + NADH
-
-
-
-
?
butan-2-ol + NAD+
butan-2-one + NADH
-
(R)-2-butanol and (S)-2-butanol
-
?
butan-2-ol + NAD+
butan-2-one + NADH
-
-
-
-
?
butan-2-ol + NAD+
butan-2-one + NADH
-
-
-
-
?
butan-2-ol + NAD+
butan-2-one + NADH
-
-
-
-
?
butan-2-ol + NAD+
butan-2-one + NADH
-
-
-
-
?
butan-2-ol + NAD+
butan-2-one + NADH
-
-
-
-
?
butan-2-ol + NAD+
butan-2-one + NADH
-
-
-
-
?
butan-2-ol + NAD+
butan-2-one + NADH
Sporotrichum pulverulentum
-
-
-
-
?
butan-2-ol + NAD+
butan-2-one + NADH
-
-
-
-
?
butan-2-ol + NAD+
butan-2-one + NADH
-
no activity
-
-
-
butan-2-ol + NAD+
butan-2-one + NADH
-
weak activity
-
-
?
butan-2-one + NADH

butan-2-ol + NAD+
-
-
-
r
butan-2-one + NADH
butan-2-ol + NAD+
-
-
-
-
r
butanol + NAD+

1-butanal + NADH + H+
about 15% of the activity with ethanol or 1-propanol
-
-
?
butanol + NAD+
1-butanal + NADH + H+
about 15% of the activity with ethanol or 1-propanol
-
-
?
butanol + NAD+

butyraldehyde + NADH
-
-
-
-
?
butanol + NAD+
butyraldehyde + NADH
-
-
-
-
?
butanol + NAD+
butyraldehyde + NADH
-
-
-
-
?
butanol + NAD+
butyraldehyde + NADH
-
-
-
-
?
butanol + NAD+
butyraldehyde + NADH
-
-
-
-
?
butanol + NAD+
butyraldehyde + NADH
-
-
-
-
?
butanol + NAD+
butyraldehyde + NADH
-
-
-
-
?
butanol + NAD+
butyraldehyde + NADH
-
-
-
-
?
butanol + NAD+
butyraldehyde + NADH
-
-
-
-
?
butanol + NAD+
butyraldehyde + NADH
-
-
-
-
?
butanol + NAD+
butyraldehyde + NADH
-
-
-
-
?
butanol + NAD+
butyraldehyde + NADH
-
-
-
-
?
butanol + NAD+
butyraldehyde + NADH
-
-
-
-
?
butanol + NAD+
butyraldehyde + NADH
-
pH 10.0: oxidized by ADH-1 and ADH-3, no activity with isoenzyme ADH-2
-
-
?
butanol + NAD+
butyraldehyde + NADH
-
-
-
-
?
butanol + NAD+
butyraldehyde + NADH
-
weak
-
-
-
butanol + NAD+
butyraldehyde + NADH
-
-
-
-
?
butanol + NAD+
butyraldehyde + NADH
-
-
-
-
?
butanol + NAD+
butyraldehyde + NADH
-
-
-
-
?
butanol + NAD+
butyraldehyde + NADH
-
-
-
-
?
butanol + NAD+
butyraldehyde + NADH
-
-
-
-
?
butanol + NAD+
butyraldehyde + NADH
-
activity with ADH I, no activity with ADH II
-
-
?
butanol + NAD+
butyraldehyde + NADH
-
oxidized by enzyme form ADH-I, no activity with enzyme form ADH-II
-
-
-
butanol + NAD+

butyraldehyde + NADH + H+
-
immobilized HLAD shows about 95% reaction rate and free HLAD shows about 90% reaction rate with butanol compared to ethanol at pH 8.8 and 30°C
-
-
?
butanol + NAD+
butyraldehyde + NADH + H+
-
359% activity compared to cyclohexanone
-
r
butyraldehyde + NADH

n-butanol + NAD+
-
-
-
-
r
butyraldehyde + NADH
n-butanol + NAD+
-
-
-
-
r
butyraldehyde + NADH
n-butanol + NAD+
-
activity with enzyme form ADH I, no activity with enzyme form ADH II
-
r
butyraldehyde + NADH + H+

n-butanol + NAD+
-
best substrate for isozyme ADH II
-
-
r
butyraldehyde + NADH + H+
n-butanol + NAD+
-
best substrate for isozyme ADH II
-
-
r
butyraldehyde + NADH + H+
n-butanol + NAD+
-
-
-
-
?
butyraldehyde + NADH + H+
n-butanol + NAD+
-
-
-
-
?
capronaldehyde + NADH

1-hexanol + NAD+
-
best substrate
-
-
r
capronaldehyde + NADH
1-hexanol + NAD+
-
best substrate
-
-
r
capronaldehyde + NADH
1-hexanol + NAD+
-
best substrate
-
-
r
chloroacetone + NADH + H+

1-chloropropan-2-ol + NAD+
-
41.7fold higher activity compared to activity with N-benzyl-3-pyrrolidinone
-
-
?
chloroacetone + NADH + H+
1-chloropropan-2-ol + NAD+
-
238% of the activity with phenyl trifluoromethyl ketone
-
-
?
cinnamyl alcohol + NAD+

cinnamaldehyde + NADH
-
-
-
-
?
cinnamyl alcohol + NAD+
cinnamaldehyde + NADH
-
-
-
-
?
cinnamyl alcohol + NAD+
cinnamaldehyde + NADH
-
-
-
-
?
crotyl alcohol + NAD+

crotonaldehyde + NADH
-
-
-
-
?
crotyl alcohol + NAD+
crotonaldehyde + NADH
-
product is toxic in mouse hepatocytes due to cell protein carbonylation following exposure to crotyl alcohol
-
-
?
crotyl alcohol + NAD+
crotonaldehyde + NADH
-
-
product is toxic in mouse hepatocytes due to cell protein carbonylation following exposure to crotyl alcohol
-
?
crotyl alcohol + NAD+
crotonaldehyde + NADH
-
-
-
-
?
cycloheptanol + NAD+

cycloheptanone + NADH + H+
-
-
-
?
cycloheptanol + NAD+
cycloheptanone + NADH + H+
-
-
-
r
cycloheptanol + NAD+
cycloheptanone + NADH + H+
37% of activity compared to (S)-1-indanol
-
-
?
cycloheptanone + NADH

cycloheptanol + NAD+
-
-
-
-
?
cycloheptanone + NADH
cycloheptanol + NAD+
-
-
-
-
?
cyclohexanol + NAD+

cyclohexanone + NADH
-
-
-
-
?
cyclohexanol + NAD+
cyclohexanone + NADH
-
-
-
-
?
cyclohexanol + NAD+
cyclohexanone + NADH
-
-
-
-
?
cyclohexanol + NAD+
cyclohexanone + NADH
-
reaction is catalyzed by the cathodic pyrazole-sensitive enzyme, no activity with the cathodic pyrazole-insensitive enzyme and by the anodic pyrazole-insensitive enzyme
-
-
?
cyclohexanol + NAD+
cyclohexanone + NADH
-
-
-
-
?
cyclohexanol + NAD+
cyclohexanone + NADH
-
oxidized by isoenzyme A2, no activity with isoenzymes B2 and C2
-
-
?
cyclohexanol + NAD+
cyclohexanone + NADH
-
-
-
-
?
cyclohexanol + NAD+
cyclohexanone + NADH
-
-
-
-
?
cyclohexanol + NAD+
cyclohexanone + NADH
-
-
-
-
?
cyclohexanol + NAD+
cyclohexanone + NADH
-
-
-
-
?
cyclohexanol + NAD+
cyclohexanone + NADH
-
no activity
-
-
-
cyclohexanol + NAD+

cyclohexanone + NADH + H+
-
-
-
-
?
cyclohexanol + NAD+
cyclohexanone + NADH + H+
12% of the activity with 2,3-butanediol
-
-
r
cyclohexanol + NAD+
cyclohexanone + NADH + H+
-
-
-
-
?
cyclohexanol + NAD+
cyclohexanone + NADH + H+
-
-
-
-
r
cyclohexanol + NAD+
cyclohexanone + NADH + H+
-
-
-
-
?
cyclohexanol + NAD+
cyclohexanone + NADH + H+
52% of the activity with 1-phenylethanol
-
-
?
cyclohexanol + NAD+
cyclohexanone + NADH + H+
100% activity
100% activity
-
r
cyclohexanol + NAD+
cyclohexanone + NADH + H+
-
13% of the activity with (S)-(-)-1-phenylethanol
-
-
?
cyclohexanone + NADH

cyclohexanol + NAD+
-
-
-
-
?
cyclohexanone + NADH
cyclohexanol + NAD+
-
best cyclic ketone substrate
-
-
?
cyclohexanone + NADH + H+

cyclohexanol + NAD+
-
-
-
-
?
cyclohexanone + NADH + H+
cyclohexanol + NAD+
38% of the activity with acetoin
-
-
r
cyclohexanone + NADH + H+
cyclohexanol + NAD+
-
-
-
?
cyclohexanone + NADH + H+
cyclohexanol + NAD+
-
-
-
?
cyclopentanol + NAD+

? + NADH
-
-
-
-
?
cyclopentanol + NAD+
? + NADH
-
-
-
-
?
cyclopentanol + NAD+

cyclopentanone + NADH + H+
-
-
92% of the activity with butan-2-ol
-
?
cyclopentanol + NAD+
cyclopentanone + NADH + H+
55% of the activity compared to isoborneol
-
-
?
D-glucitol + NAD+

? + NADH
-
-
-
-
?
D-glucitol + NAD+
? + NADH
-
-
-
-
?
diacetyl + NADH + H+

?
-
16.1fold higher activity compared to activity with N-benzyl-3-pyrrolidinone
-
-
?
diacetyl + NADH + H+
?
-
16.1fold higher activity compared to activity with N-benzyl-3-pyrrolidinone
-
-
?
ethanol + 2 NAD+ + H2O

acetic acid + 2 NADH + 2 H+
best substrate
-
-
r
ethanol + 2 NAD+ + H2O
acetic acid + 2 NADH + 2 H+
about 45% of the activity with 1-octanol
-
-
r
ethanol + NAD+

acetaldehyde + NADH
-
-
acetaldehyde is the best substrate for isozyme ADH I
-
r
ethanol + NAD+
acetaldehyde + NADH
-
-
acetaldehyde is the best substrate for isozyme ADH I
-
r
ethanol + NAD+
acetaldehyde + NADH
-
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH
-
-
-
-
r
ethanol + NAD+
acetaldehyde + NADH
-
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH
-
76% of the activity with 2-phenylethanol
-
-
?
ethanol + NAD+
acetaldehyde + NADH
-
76% of the activity with 2-phenylethanol
-
-
?
ethanol + NAD+
acetaldehyde + NADH
-
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH
-
the reduction of acetaldehyde of ADH-MII is about 7times higher than that of the oxidation of ethanol
-
r
ethanol + NAD+
acetaldehyde + NADH
-
the reduction of acetaldehyde of ADH-MII is about 7times higher than that of the oxidation of ethanol
-
r
ethanol + NAD+
acetaldehyde + NADH
-
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH
-
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH
-
no cooperativity between the 2 active sites of the enzyme
-
-
r
ethanol + NAD+
acetaldehyde + NADH
-
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH
-
the reduction of acetaldehyde is 4.9fold faster than the oxidation of ethanol
-
r
ethanol + NAD+
acetaldehyde + NADH
-
-
-
-
-
ethanol + NAD+
acetaldehyde + NADH
-
-
-
-
r
ethanol + NAD+
acetaldehyde + NADH
-
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH
-
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH
-
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH
-
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH
-
-
-
-
-
ethanol + NAD+
acetaldehyde + NADH
-
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH
-
-
-
r
ethanol + NAD+
acetaldehyde + NADH
-
-
-
r
ethanol + NAD+
acetaldehyde + NADH
-
-
-
r
ethanol + NAD+
acetaldehyde + NADH
-
plays an important role in the metabolism of ethanol
-
-
?
ethanol + NAD+
acetaldehyde + NADH
isomerization of the enzyme-NAD+ complex is the rate-limiting step for acetaldehyde reduction by the wild-type enzyme
-
-
r
ethanol + NAD+
acetaldehyde + NADH
-
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH
-
-
-
-
r
ethanol + NAD+
acetaldehyde + NADH
-
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH
-
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH
proton and hydride equivalent transfer in the alcohol dehydrogenase enzymatic reaction are modulated by the correlated motions between NAD+ and the cofactor domain
-
-
?
ethanol + NAD+
acetaldehyde + NADH
-
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH
-
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH
-
-
285565, 285567, 285568, 285569, 285572, 285573, 285575, 285608, 285651, 655197, 655206, 655479 -
-
?
ethanol + NAD+
acetaldehyde + NADH
-
class III isoenzyme chi-ADH oxidizes ethanol very poorly
-
-
?
ethanol + NAD+
acetaldehyde + NADH
-
no oxidation with class III enzyme
-
-
-
ethanol + NAD+
acetaldehyde + NADH
-
chi-ADH plays an important role in the metabolism of long chain alcohols and aldehydes
-
-
?
ethanol + NAD+
acetaldehyde + NADH
-
the anodic enzyme form may contribute significantly to alcohol elimination in man, particularly at high concentrations when the other enzyme species are saturated
-
-
?
ethanol + NAD+
acetaldehyde + NADH
-
the enzyme plays a significant role in first-pass metabolism of ethanol in human
-
-
?
ethanol + NAD+
acetaldehyde + NADH
-
enzyme is responsible for the major ethanol oxidation capacity in the body. The products acetaldehyde and NADH are responsible for the most of the toxic effects and metabolic disturbances produced by ethanol ingestion
-
?
ethanol + NAD+
acetaldehyde + NADH
-
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH
-
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH
-
-
-
r
ethanol + NAD+
acetaldehyde + NADH
-
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH
-
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH
-
no activity with isoenzyme B2, oxidized by isoenzyme A2 and C2
-
-
?
ethanol + NAD+
acetaldehyde + NADH
-
role of the major liver isoenzyme A2 in ethanol metabolism
-
-
?
ethanol + NAD+
acetaldehyde + NADH
-
DH3 plays an important role in systemic ethanol metabolism at higher levels of blood ethanol through activation by cytoplasmic solution hydrophobicity
-
-
?
ethanol + NAD+
acetaldehyde + NADH
-
-
-
-
r
ethanol + NAD+
acetaldehyde + NADH
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH
-
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH
-
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH
-
-
-
r
ethanol + NAD+
acetaldehyde + NADH
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH
-
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH
-
-
-
-
-
ethanol + NAD+
acetaldehyde + NADH
-
-
-
-
r
ethanol + NAD+
acetaldehyde + NADH
-
isoenzyme ADH-1 and ADH-3, no activity with isoenzyme ADH-2
-
-
ethanol + NAD+
acetaldehyde + NADH
-
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH
-
-
-
-
-
ethanol + NAD+
acetaldehyde + NADH
-
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH
-
-
-
r
ethanol + NAD+
acetaldehyde + NADH
-
-
-
-
r
ethanol + NAD+
acetaldehyde + NADH
-
rate-limiting step of the alcoholic fermentation
-
-
?
ethanol + NAD+
acetaldehyde + NADH
-
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH
Sporotrichum pulverulentum
-
-
-
r
ethanol + NAD+
acetaldehyde + NADH
-
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH
-
best substrate
-
-
r
ethanol + NAD+
acetaldehyde + NADH
-
-
-
-
-
ethanol + NAD+
acetaldehyde + NADH
-
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH
-
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH
-
-
-
-
-
ethanol + NAD+
acetaldehyde + NADH
-
-
-
-
r
ethanol + NAD+

acetaldehyde + NADH + H+
-
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH + H+
-
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH + H+
-
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH + H+
-
-
-
r
ethanol + NAD+
acetaldehyde + NADH + H+
-
no activity with NADP+, in reverse reaction no activity with NADPH
-
-
r
ethanol + NAD+
acetaldehyde + NADH + H+
-
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH + H+
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH + H+
-
-
-
r
ethanol + NAD+
acetaldehyde + NADH + H+
-
-
-
-
r
ethanol + NAD+
acetaldehyde + NADH + H+
-
-
83% of the activity with butan-2-ol
-
r
ethanol + NAD+
acetaldehyde + NADH + H+
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH + H+
-
-
-
r
ethanol + NAD+
acetaldehyde + NADH + H+
-
-
-
r
ethanol + NAD+
acetaldehyde + NADH + H+
the enzyme shows a preference for short-chain alcohols ethanol and 1-propanol
-
-
?
ethanol + NAD+
acetaldehyde + NADH + H+
the enzyme shows a preference for short-chain alcohols ethanol and 1-propanol
-
-
?
ethanol + NAD+
acetaldehyde + NADH + H+
-
-
-
-
r
ethanol + NAD+
acetaldehyde + NADH + H+
substrate for isozyme ADH1C, extremely poor substrate for isozyme ADH3
-
-
r
ethanol + NAD+
acetaldehyde + NADH + H+
substrate for isozyme ADH2
-
-
r
ethanol + NAD+
acetaldehyde + NADH + H+
substrate for isozyme ADH4
-
-
r
ethanol + NAD+
acetaldehyde + NADH + H+
-
-
-
r
ethanol + NAD+
acetaldehyde + NADH + H+
-
-
-
r
ethanol + NAD+
acetaldehyde + NADH + H+
-
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH + H+
-
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH + H+
-
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH + H+
-
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH + H+
-
the enzyme is highly specific for ethanol with NAD+ as the coenzyme
-
-
r
ethanol + NAD+
acetaldehyde + NADH + H+
-
-
-
-
r
ethanol + NAD+
acetaldehyde + NADH + H+
-
best substrate
-
-
r
ethanol + NAD+
acetaldehyde + NADH + H+
-
-
-
r
ethanol + NAD+
acetaldehyde + NADH + H+
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH + H+
-
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH + H+
-
-
-
-
r
ethanol + NAD+
acetaldehyde + NADH + H+
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH + H+
-
-
-
-
-
ethanol + NAD+
acetaldehyde + NADH + H+
-
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH + H+
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH + H+
-
-
-
-
r
ethanol + NAD+
acetaldehyde + NADH + H+
-
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH + H+
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH + H+
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH + H+
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH + H+
-
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH + H+
-
-
-
-
r
ethanol + NAD+
acetaldehyde + NADH + H+
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH + H+
-
-
-
?
ethanol + NAD+
acetaldehyde + NADH + H+
100% activity
-
-
?
ethanol + NAD+
acetaldehyde + NADH + H+
88% activity compared to cyclohexanol
-
-
r
ethanol + NAD+
acetaldehyde + NADH + H+
-
-
-
-
?
ethanol + NADP+

acetaldehyde + NADPH + H+
8.5% of the activity with ethanol and NAD+
-
-
?
ethanol + NADP+
acetaldehyde + NADPH + H+
-
-
-
-
r
ethyl benzoylformate + NADH + H+

ethyl (R)-mandelate + NAD+
-
50% enantiomeric excess
-
r
ethyl benzoylformate + NADH + H+
ethyl (R)-mandelate + NAD+
-
50% enantiomeric excess
-
r
ethyl pyruvate + NADH

?
about 15% of the activity compared to isatin
-
-
?
ethyl pyruvate + NADH
?
about 15% of the activity compared to isatin
-
-
?
ethylene glycol + NAD+

?
-
poor substrate
-
-
r
ethylene glycol + NAD+
?
-
poor substrate
-
-
r
ethylene glycol + NAD+

? + NADH
-
no activity
-
-
-
ethylene glycol + NAD+
? + NADH
-
-
-
-
?
ethylene glycol + NAD+
? + NADH
-
class III isoenzyme chi-ADH shows no activity
-
-
-
ethylene glycol + NAD+
? + NADH
-
-
-
-
?
ethylene glycol + NAD+
? + NADH
-
-
-
-
?
ethylene glycol + NAD+
? + NADH
-
oxidized by isoenzyme A2, no activity with isoenzyme B2 and C2
-
-
?
formaldehyde + NADH + H+

methanol + NAD+
-
-
-
-
?
formaldehyde + NADH + H+
methanol + NAD+
-
reduced by isoenzyme A2, no activity with isoenzyme B2 and C2
-
-
r
formaldehyde + NADH + H+
methanol + NAD+
-
-
-
-
?
formaldehyde + NADH + H+
methanol + NAD+
-
activity with ADH I, no activity with ADH II
-
-
?
furfuryl alcohol + NAD+

furfural + NADH
-
-
-
-
?
furfuryl alcohol + NAD+
furfural + NADH
-
activity with ADH I, no activity with ADH II
-
r
glycerol + NAD+

dihydroxyacetone + NADH
-
-
-
-
?
glycerol + NAD+
dihydroxyacetone + NADH
-
-
-
-
?
hexan-1-ol + NAD+

n-hexanal + NADH
-
-
-
-
?
hexan-1-ol + NAD+
n-hexanal + NADH
-
-
-
-
?
hexan-1-ol + NAD+
n-hexanal + NADH
-
-
-
-
?
hexan-1-ol + NAD+
n-hexanal + NADH
-
-
-
-
?
hexan-1-ol + NAD+
n-hexanal + NADH
-
-
-
-
?
hexan-1-ol + NAD+
n-hexanal + NADH
-
-
-
-
?
hexanol + NAD+

n-hexanal + NADH
-
-
-
-
?
hexanol + NAD+
n-hexanal + NADH
-
-
-
?
hexanol + NAD+
n-hexanal + NADH
-
-
-
-
?
hexanol + NAD+
n-hexanal + NADH
-
-
-
-
-
hexanol + NAD+
n-hexanal + NADH
-
-
-
-
?
hexanol + NAD+
n-hexanal + NADH
-
-
-
-
-
hexanol + NAD+
n-hexanal + NADH
-
-
-
-
?
hexanol + NAD+
n-hexanal + NADH
Sporotrichum pulverulentum
-
-
-
-
?
hexanol + NAD+
n-hexanal + NADH
-
activity with ADH I, no activity with ADH II
-
-
-
isatin + NADH + H+

? + NAD+
-
-
-
r
isatin + NADH + H+
? + NAD+
-
-
-
r
iso-propanol + NAD+

isopropanal + NADH + H+
-
-
-
?
iso-propanol + NAD+
isopropanal + NADH + H+
-
-
-
?
isopropanol + 2 NAD+ + H2O

? + 2 NADH + 2 H+
about 10% of the activity with 1-octanol
-
-
r
isopropanol + 2 NAD+ + H2O
? + 2 NADH + 2 H+
about 15% of the activity with 1-octanol
-
-
r
isopropanol + NAD+

acetone + NADH + H+
low affinity
-
-
?
isopropanol + NAD+
acetone + NADH + H+
-
-
-
-
?
m-nitrobenzaldehyde + NADH + H+

m-nitrobenzyl alcohol + NAD+
-
substrate of class IV ADH isozyme
-
-
r
m-nitrobenzaldehyde + NADH + H+
m-nitrobenzyl alcohol + NAD+
-
-
-
-
methanol + NAD+

formaldehyde + NADH + H+
-
-
-
-
?
methanol + NAD+
formaldehyde + NADH + H+
-
reaction is extremly weak
-
-
?
methanol + NAD+
formaldehyde + NADH + H+
-
reaction is extremly weak
-
-
?
methanol + NAD+
formaldehyde + NADH + H+
-
-
-
-
?
methanol + NAD+
formaldehyde + NADH + H+
-
no activity
-
-
-
methanol + NAD+
formaldehyde + NADH + H+
-
-
-
-
ir
methanol + NAD+
formaldehyde + NADH + H+
-
-
-
-
?
methanol + NAD+
formaldehyde + NADH + H+
-
no activity
-
-
-
methanol + NAD+
formaldehyde + NADH + H+
-
anodic enzyme form shows no activity
-
-
-
methanol + NAD+
formaldehyde + NADH + H+
-
class I isoenzymes
-
-
?
methanol + NAD+
formaldehyde + NADH + H+
-
no activity with isoenzyme beta3,beta3
-
-
?
methanol + NAD+
formaldehyde + NADH + H+
-
activity detected with class II isoenzyme pi-ADH
-
-
-
methanol + NAD+
formaldehyde + NADH + H+
-
oxidized by isoenzyme beta1,beta1
-
-
?
methanol + NAD+
formaldehyde + NADH + H+
-
class III isoenzyme chi-ADH shows no activity
-
-
-
methanol + NAD+
formaldehyde + NADH + H+
-
no activity with class III enzyme
-
-
-
methanol + NAD+
formaldehyde + NADH + H+
-
reaction is catalyzed by the cathodic pyrazole-sensitive enzyme, no activity by the cathodic pyrazole-insensitive enzyme and by the anodic pyrazole-insensitive enzyme
-
-
?
methanol + NAD+
formaldehyde + NADH + H+
-
-
-
-
r
methanol + NAD+
formaldehyde + NADH + H+
-
no activity
-
-
-
methanol + NAD+
formaldehyde + NADH + H+
-
no activity
-
-
-
methanol + NAD+
formaldehyde + NADH + H+
-
weak activity with isoenzyme A2, no activity with isoenzyme B2 and C2
-
-
?, r
methanol + NAD+
formaldehyde + NADH + H+
-
-
-
-
?
methanol + NAD+
formaldehyde + NADH + H+
-
no activity at pH 7.5, slight activity at pH 10.8
-
-
?
methanol + NAD+
formaldehyde + NADH + H+
-
-
-
?
methanol + NAD+
formaldehyde + NADH + H+
-
oxidized with ADH-3, no activity with ADH-1 and ADH-2
-
-
?
methanol + NAD+
formaldehyde + NADH + H+
-
reaction is catalyzed by the pyrazole-sensitive enzyme, no activity with the pyrazole-insensitive enzyme
-
-
?
methanol + NAD+
formaldehyde + NADH + H+
Sporotrichum pulverulentum
-
no activity
-
-
-
methanol + NAD+
formaldehyde + NADH + H+
-
low activity
-
-
?
methanol + NAD+
formaldehyde + NADH + H+
49.9% activity compared to ethanol
-
-
?
methanol + NAD+
formaldehyde + NADH + H+
-
no activity
-
-
-
methanol + NAD+
formaldehyde + NADH + H+
-
no activity
-
-
-
methyl benzoylformate + NADH

?
about 35% of the activity compared to isatin
-
-
?
methyl benzoylformate + NADH
?
about 35% of the activity compared to isatin
-
-
?
N-benzyl-3-pyrrolidinone + NADH + H+

(S)-N-benzyl-3-pyrrolidinol + NAD+
-
-
-
-
r
N-benzyl-3-pyrrolidinone + NADH + H+
(S)-N-benzyl-3-pyrrolidinol + NAD+
-
(S)-N-benzyl-3-pyrrolidinol is produced at an enantiomeric excess of more than 99.9%
-
-
r
n-butanol + NAD+

butylaldehyde + NADH + H+
-
-
-
-
?
n-butanol + NAD+
butylaldehyde + NADH + H+
-
-
-
-
r
n-butanol + NAD+

n-butanal + NADH
-
-
-
-
?
n-butanol + NAD+
n-butanal + NADH
-
-
-
-
r
n-propanol + NAD+

propanal + NADH
-
-
-
?
n-propanol + NAD+
propanal + NADH
-
-
-
-
?
n-propanol + NAD+

propanal + NADH + H+
-
-
-
-
r
n-propanol + NAD+
propanal + NADH + H+
-
-
-
?
n-propanol + NAD+
propanal + NADH + H+
-
-
-
?
octan-1-ol + NAD+

n-octanal + NADH
-
-
-
-
?
octan-1-ol + NAD+
n-octanal + NADH
-
-
-
-
?
octan-1-ol + NAD+
n-octanal + NADH
-
-
-
-
?
octan-1-ol + NAD+
n-octanal + NADH
-
-
-
-
?
octan-1-ol + NAD+
n-octanal + NADH
-
-
-
-
?
octan-1-ol + NAD+
n-octanal + NADH
-
-
-
-
?
octan-1-ol + NAD+
n-octanal + NADH
-
-
-
-
?
octan-1-ol + NAD+
n-octanal + NADH
Sporotrichum pulverulentum
-
weak activity
-
-
?
octan-1-ol + NAD+
n-octanal + NADH
-
-
-
-
?
octan-1-ol + NAD+
n-octanal + NADH
-
activity with ADH I, no activity with ADH II
-
-
?
octan-2-ol + NAD+

octan-2-one + NADH
-
(R)-2-octanol and (S)-2-octanol
-
-
?
octan-2-ol + NAD+
octan-2-one + NADH
-
-
-
-
?
octan-2-ol + NAD+
octan-2-one + NADH
-
-
-
-
?
octan-2-ol + NAD+
octan-2-one + NADH
Sporotrichum pulverulentum
-
weak activity
-
-
?
octanal + NAD+ + H2O

octanoic acid + NADH + H+
about 25% of the activity with 1-octanol
-
-
r
octanal + NAD+ + H2O
octanoic acid + NADH + H+
about 25% of the activity with 1-octanol
-
-
r
octanal + NADH + H+

octanol + NAD+
-
-
-
-
?
octanal + NADH + H+
octanol + NAD+
-
-
-
-
?
octanal + NADH + H+
octanol + NAD+
-
-
-
-
?
octanol + NAD+

octanal + NADH
-
-
-
-
?
octanol + NAD+
octanal + NADH
-
-
-
?
octanol + NAD+
octanal + NADH
-
-
-
-
?
octanol + NAD+

octanal + NADH + H+
-
-
-
?
octanol + NAD+
octanal + NADH + H+
-
immobilized and free HLAD show 100% reaction rate with octanol compared to ethanol at pH 8.8 and 30°C
-
-
?
octanol + NAD+
octanal + NADH + H+
-
-
-
?
octanol + NAD+
octanal + NADH + H+
-
-
-
-
?
octanol + NAD+
octanal + NADH + H+
-
178% activity compared to cyclohexanone
-
r
p-methoxybenzyl alcohol + NAD+

p-methoxybenzaldehyde + NADH + H+
-
-
-
r
p-methoxybenzyl alcohol + NAD+
p-methoxybenzaldehyde + NADH + H+
-
-
-
-
r
pentan-2-ol + NAD+

2-pentanone + NADH
-
(R)-2-pentanol and (S)-2-pentanol
-
r
pentan-2-ol + NAD+
2-pentanone + NADH
-
-
-
-
?
pentan-2-ol + NAD+
2-pentanone + NADH
-
-
-
-
?
pentan-2-ol + NAD+
2-pentanone + NADH
Sporotrichum pulverulentum
-
-
-
-
?
pentan-2-ol + NAD+
2-pentanone + NADH
-
-
-
-
?
pentan-2-ol + NAD+

pentan-2-one + NADH + H+
-
-
93% of the activity with butan-2-ol
-
?
pentan-2-ol + NAD+
pentan-2-one + NADH + H+
-
9% of the activity with 2,3-butanediol
-
?
pentan-3-ol + NAD+

3-pentanone + NADH
-
-
-
r
pentan-3-ol + NAD+
3-pentanone + NADH
-
-
-
-
?
pentanol + NAD+

n-pentanal + NADH
-
-
-
-
?
pentanol + NAD+
n-pentanal + NADH
-
-
-
-
?
pentanol + NAD+
n-pentanal + NADH
-
-
-
-
?
pentanol + NAD+
n-pentanal + NADH
-
-
-
-
?
pentanol + NAD+
n-pentanal + NADH
-
-
-
-
?
pentanol + NAD+
n-pentanal + NADH
-
-
-
-
?
pentanol + NAD+
n-pentanal + NADH
-
-
-
-
?
pentanol + NAD+
n-pentanal + NADH
-
-
-
-
?
pentanol + NAD+
n-pentanal + NADH
-
-
-
-
?
pentanol + NAD+
n-pentanal + NADH
-
-
-
-
?
pentanol + NAD+
n-pentanal + NADH
-
-
-
-
?
pentanol + NAD+
n-pentanal + NADH
-
activity with ADH I, no activity with ADH II
-
-
?
pentanol + NAD+

pentanal + NADH + H+
-
-
-
?
pentanol + NAD+
pentanal + NADH + H+
-
-
-
-
?
pentanol + NAD+

pentanone + NADH
-
-
-
-
?
pentanol + NAD+
pentanone + NADH
-
-
-
-
?
pentanol + NAD+
pentanone + NADH
-
-
-
-
?
pentanol + NAD+
pentanone + NADH
-
oxidized by isoenzyme A2 and B2, no activity with isoenzyme C2
-
-
?
pentanol + NAD+
pentanone + NADH
-
-
-
-
?
pentanol + NAD+
pentanone + NADH
Sporotrichum pulverulentum
-
-
-
-
?
phenylacetaldehyde + NADH

phenylethanol + NAD+
-
-
-
-
r
phenylacetaldehyde + NADH
phenylethanol + NAD+
-
-
-
-
r
phenylacetaldehyde + NADH
phenylethanol + NAD+
-
-
-
-
r
primary or secondary alcohol + NAD+

aldehyde or ketone + NADH
-
-
-
-
r
primary or secondary alcohol + NAD+
aldehyde or ketone + NADH
-
-
-
-
r
propan-1,2-diol + NAD+

? + NADH
-
-
-
-
?
propan-1,2-diol + NAD+
? + NADH
-
-
-
-
?
propan-1-ol + NAD+

propanal + NADH + H+
-
-
-
?
propan-1-ol + NAD+
propanal + NADH + H+
-
-
-
?
propan-2-ol + NAD+

acetone + NADH
-
-
-
-
?
propan-2-ol + NAD+
acetone + NADH
-
3% of the activity with ethanol
-
-
-
propan-2-ol + NAD+
acetone + NADH
-
-
-
-
?
propan-2-ol + NAD+
acetone + NADH
-
-
-
-
?
propan-2-ol + NAD+
acetone + NADH
-
-
-
-
?
propan-2-ol + NAD+
acetone + NADH
-
-
-
-
?
propan-2-ol + NAD+
acetone + NADH
-
-
-
-
?
propan-2-ol + NAD+
acetone + NADH
-
-
-
-
?
propan-2-ol + NAD+
acetone + NADH
-
-
-
-
?
propan-2-ol + NAD+
acetone + NADH
-
-
-
-
?
propan-2-ol + NAD+
acetone + NADH
-
-
-
-
?
propan-2-ol + NAD+
acetone + NADH
-
no activity
-
-
-
propan-2-ol + NAD+
acetone + NADH
-
-
-
-
?
propan-2-ol + NAD+
acetone + NADH
Sporotrichum pulverulentum
-
-
-
-
?
propan-2-ol + NAD+
acetone + NADH
-
-
-
-
?
propan-2-ol + NAD+
acetone + NADH
-
-
-
-
?
propan-2-ol + NAD+
acetone + NADH
-
-
-
-
-
propan-2-ol + NAD+
acetone + NADH
-
very low activity
-
-
-
propan-2-ol + NAD+

acetone + NADH + H+
-
-
synthesis of [4R-(2)H]NADH with high yield by enzymatic oxidation of 2-propanol-d(8)
-
?
propan-2-ol + NAD+
acetone + NADH + H+
-
6% activity compared to cyclohexanone
-
r
propanol + NADH

propionaldehyde + NADH
-
-
-
-
?
propanol + NADH
propionaldehyde + NADH
-
-
-
-
?
propanol + NADH
propionaldehyde + NADH
-
-
-
-
?
propanol + NADH
propionaldehyde + NADH
-
-
-
-
?
propanol + NADH
propionaldehyde + NADH
-
-
-
-
?
propanol + NADH
propionaldehyde + NADH
-
-
-
-
?
propanol + NADH
propionaldehyde + NADH
-
-
-
-
?
propanol + NADH
propionaldehyde + NADH
-
-
-
-
?
propanol + NADH
propionaldehyde + NADH
-
-
-
-
?
propanol + NADH
propionaldehyde + NADH
-
-
-
-
?
propanol + NADH
propionaldehyde + NADH
-
-
-
-
?
propanol + NADH
propionaldehyde + NADH
Sporotrichum pulverulentum
-
-
-
-
?
propanol + NADH
propionaldehyde + NADH
-
-
-
-
?
propanol + NADH
propionaldehyde + NADH
-
-
-
-
?
propanol + NADH
propionaldehyde + NADH
-
-
-
-
?
propanol + NADH
propionaldehyde + NADH
-
-
-
-
?
propanol + NADH
propionaldehyde + NADH
-
-
-
-
-
propanol + NADH
propionaldehyde + NADH
-
-
-
-
propionaldehyde + NADH

propanol + NAD+
-
-
-
-
?
propionaldehyde + NADH
propanol + NAD+
-
-
-
-
?
propionaldehyde + NADH
propanol + NAD+
-
94% of the activity with acetaldehyde
-
-
?
propionaldehyde + NADH
propanol + NAD+
-
-
-
-
?
trans-cinnamaldehyde + NADH + H+

cinnamyl alcohol + NAD+
-
% of the activity with phenyl trifluoromethyl ketone
-
-
?
trans-cinnamaldehyde + NADH + H+
cinnamyl alcohol + NAD+
-
-
-
-
r
valeraldehyde + NADH

1-pentanol + NAD+
-
-
-
-
r
valeraldehyde + NADH
1-pentanol + NAD+
-
-
-
-
r
valeraldehyde + NADH
1-pentanol + NAD+
-
-
-
-
r
additional information

?
-
-
substrate specificity of the 2 isozmyes with various substrates, overview, isozymes are highly specific for the (R)-stereoisomers and enantioselctive for the R(-)isomers
-
-
-
additional information
?
-
-
substrate specificity of the 2 isozmyes with various substrates, overview, isozymes are highly specific for the (R)-stereoisomers and enantioselctive for the R(-)isomers
-
-
-
additional information
?
-
-
the enzyme shows broad substrate specificity and prefers aliphatic alcohols and ketones. There are no large differences in the reactivities between primary and secondary alcohols. The enzyme produces (S)-alcohols from the corresponding ketones. The values of the enantiomeric excess increase with the increase of chain length except for the reduction of 2-hexanone. The highest enantioselectivity is shown with the reduction of 2-nonanone
-
-
-
additional information
?
-
-
the enzyme shows broad substrate specificity and prefers aliphatic alcohols and ketones. There are no large differences in the reactivities between primary and secondary alcohols. The enzyme produces (S)-alcohols from the corresponding ketones. The values of the enantiomeric excess increase with the increase of chain length except for the reduction of 2-hexanone. The highest enantioselectivity is shown with the reduction of 2-nonanone
-
-
-
additional information
?
-
-
substrate specificity and enantiospecificity, overview. The (R)-specific alcohol dehydrogenase requires NADH and reduces various kinds of carbonyl compounds, including ketones and aldehydes. AFPDH reduces acetylpyridine derivatives, beta-keto esters, and some ketones compounds with high enantiospecificity, overview. No activity with 2-chlorobenzaldehyde and 2-tetralone, poor activity with 1-tetralone, pyruvate, 2-oxobutyrate, oxalacetate, cyclopentanone, cyclohexanone, cycloheptanone, and dipropylketone. No activity with 1,2-propanediol, 3-chloro-1,2-propanediol, 3-bromo-1,2-propanediol, glycerol, 1-pentanol, poor activity with 1-butanol, 1-propanol, ethanol, and methanol
-
-
-
additional information
?
-
-
substrate specificity and enantiospecificity, overview. The (R)-specific alcohol dehydrogenase requires NADH and reduces various kinds of carbonyl compounds, including ketones and aldehydes. AFPDH reduces acetylpyridine derivatives, beta-keto esters, and some ketones compounds with high enantiospecificity, overview. No activity with 2-chlorobenzaldehyde and 2-tetralone, poor activity with 1-tetralone, pyruvate, 2-oxobutyrate, oxalacetate, cyclopentanone, cyclohexanone, cycloheptanone, and dipropylketone. No activity with 1,2-propanediol, 3-chloro-1,2-propanediol, 3-bromo-1,2-propanediol, glycerol, 1-pentanol, poor activity with 1-butanol, 1-propanol, ethanol, and methanol
-
-
-
additional information
?
-
-
one constitutive enzyme, ADH-MI and one inducible enzyme, ADH-MII
-
-
-
additional information
?
-
-
one constitutive enzyme, ADH-MI and one inducible enzyme, ADH-MII
-
-
-
additional information
?
-
-
the enzyme exhibits broad substrate specificity towards aliphatic ketones, cycloalkanones, aromatic ketones, and ketoesters
-
-
-
additional information
?
-
-
the enzyme exhibits broad substrate specificity towards aliphatic ketones, cycloalkanones, aromatic ketones, and ketoesters
-
-
-
additional information
?
-
-
no activity with methanol, 2-propanol, and isoamyl alcohol
-
-
-
additional information
?
-
-
Cm-ADH2 cannot reduce branched aldehydes
-
-
-
additional information
?
-
-
when NADH is replaced with NADPH, the reaction rate is reduced by 0.6%
-
-
-
additional information
?
-
-
when NADH is replaced with NADPH, the reaction rate is reduced by 0.6%
-
-
-
additional information
?
-
-
no activity detected with: N-benzyl-2-pyrrolidinone, 2-pyrrolidinone, 3-hexanone, 4-hydroxy-2-butanone, (R)-N-benzyl-3-pyrrolidinol, ethanol, 1,3-propanediol, 1-butanol, 1,4-butanediol, 1,2,3-butanetriol, 1,2,4-butanetriol, acetol, 2-phenyl-1-propanol, 3-phenyl-1-propanol, benzyl alcohol and glycerol. No activity with NADP+ or NADPH
-
-
-
additional information
?
-
-
activity is severely reduced towards aliphatic alcohols of more than 8 carbon atoms for the free enzyme, but not so with immobilized HLAD, exhibiting an activity towards C22 and C24 aliphatic alcohols higher than 50% of the highest value, obtained with C8
-
-
-
additional information
?
-
no activity is detected using 1 mM NADP+
-
-
-
additional information
?
-
-
no activity with methanol
-
-
-
additional information
?
-
no activity with methanol
-
-
-
additional information
?
-
no activity with methanol
-
-
-
additional information
?
-
-
broad substrate specificity
-
-
-
additional information
?
-
-
mutation at the substrate-binding site, or at a dimer interface, alters kinetic properties and protein oligomeric structure, active site flexibility is correlated with subunit interactions 20 A away
-
-
-
additional information
?
-
substrates are a broad range of alkyl alcohols from ethanol to 1-triacontanol
-
-
-
additional information
?
-
substrates are a broad range of alkyl alcohols from ethanol to 1-triacontanol
-
-
-
additional information
?
-
substrates are a broad range of alkyl alcohols from ethanol to 1-triacontanol
-
-
-
additional information
?
-
substrates are a broad range of alkyl alcohols from ethanol to 1-triacontanol
-
-
-
additional information
?
-
-
substrates are a broad range of alkyl alcohols from ethanol to 1-triacontanol
-
-
-
additional information
?
-
-
no activity towards methanol
-
-
-
additional information
?
-
no activity towards methanol
-
-
-
additional information
?
-
no activity towards methanol
-
-
-
additional information
?
-
the NAD+-dependent HvADH1 shows a preference for short-chain alcohols, no activity with methanol
-
-
-
additional information
?
-
-
the NAD+-dependent HvADH1 shows a preference for short-chain alcohols, no activity with methanol
-
-
-
additional information
?
-
the NAD+-dependent HvADH1 shows a preference for short-chain alcohols, no activity with methanol
-
-
-
additional information
?
-
-
differences in the activities of total ADH and class I ADH isoenzyme between cancer liver tissues and healthy hepatocytes may be a factor in ethanol metabolism disorders, which can intensify carcinogenesis
-
-
-
additional information
?
-
1-octanal is no substrate for isozyme ADH1C
-
-
-
additional information
?
-
1-octanal is no substrate for isozyme ADH1C
-
-
-
additional information
?
-
1-octanal is no substrate for isozyme ADH2
-
-
-
additional information
?
-
1-octanal is no substrate for isozyme ADH2
-
-
-
additional information
?
-
1-octanal is no substrate for isozyme ADH4
-
-
-
additional information
?
-
1-octanal is no substrate for isozyme ADH4
-
-
-
additional information
?
-
-
the enzyme does not act on short-chain normal alkyl alcohols, including methanol and ethanol
-
-
-
additional information
?
-
-
the enzyme does not act on short-chain normal alkyl alcohols, including methanol and ethanol
-
-
-
additional information
?
-
-
alcohol dehydrogenase activity may not limit alcohol supply for ester production during ripening
-
-
-
additional information
?
-
-
involvement in the development of male hamster reproductive system
-
-
-
additional information
?
-
mutation at the substrate-binding site, or at a dimer interface, alters kinetic properties and protein oligomeric structure, active site flexibility is correlated with subunit interactions 20 A away
-
-
-
additional information
?
-
mutation at the substrate-binding site, or at a dimer interface, alters kinetic properties and protein oligomeric structure, active site flexibility is correlated with subunit interactions 20 A away
-
-
-
additional information
?
-
-
ADH1 preferrs primary alcohols containing C3-C8 carbons to secondary alcohols such as 2-propanol and 2-butanol. ADH1 possesses specific carboxylate ester-forming activity
-
-
-
additional information
?
-
ADH1 preferrs primary alcohols containing C3-C8 carbons to secondary alcohols such as 2-propanol and 2-butanol. ADH1 possesses specific carboxylate ester-forming activity
-
-
-
additional information
?
-
-
ADH1 preferrs primary alcohols containing C3-C8 carbons to secondary alcohols such as 2-propanol and 2-butanol. ADH1 possesses specific carboxylate ester-forming activity
-
-
-
additional information
?
-
ADH1 preferrs primary alcohols containing C3-C8 carbons to secondary alcohols such as 2-propanol and 2-butanol. ADH1 possesses specific carboxylate ester-forming activity
-
-
-
additional information
?
-
-
no activity with methanol, 1-butanol, glycerol or 2-propanol
-
-
-
additional information
?
-
-
no activity with methanol, 1-butanol, glycerol or 2-propanol
-
-
-
additional information
?
-
methanol, formaldehyde, and acetone are no substrates for HpADH3
-
-
-
additional information
?
-
methanol, formaldehyde, and acetone are no substrates for HpADH3
-
-
-
additional information
?
-
-
enzyme may be involved in the metabolism of dietary wax esters in salmonid fish
-
-
-
additional information
?
-
-
no activity towards methanol, ethanol, 1-propanol, triethylene glycol, polyethylene glycol 400, polyethylene glycol 1000, D-sorbitol, D-sorbose, formaldehyde, acetaldehyde, propionaldehyde, butyraldehyde, and valeraldehyde
-
-
-
additional information
?
-
no activity towards methanol, ethanol, 1-propanol, triethylene glycol, polyethylene glycol 400, polyethylene glycol 1000, D-sorbitol, D-sorbose, formaldehyde, acetaldehyde, propionaldehyde, butyraldehyde, and valeraldehyde
-
-
-
additional information
?
-
no activity towards methanol, ethanol, 1-propanol, triethylene glycol, polyethylene glycol 400, polyethylene glycol 1000, D-sorbitol, D-sorbose, formaldehyde, acetaldehyde, propionaldehyde, butyraldehyde, and valeraldehyde
-
-
-
additional information
?
-
-
broad substrate specificity with a preference for the reduction of ketones and the oxidation of secondary alcohols
-
-
-
additional information
?
-
broad substrate specificity with a preference for the reduction of ketones and the oxidation of secondary alcohols
-
-
-
additional information
?
-
-
constitutive enzyme
-
-
-
additional information
?
-
-
substrate specificity and stereospecificity, substrate binding pocket structure of the 3 isozymes, involving Met294, Trp57, and Trp93
-
-
-
additional information
?
-
-
effects of pressure on deuterium isotope effects of yeast alcohol dehydrogenase using alternative substrates
-
-
-
additional information
?
-
-
the enzyme oxidizes alcohols to aldehydes or ketones both for detoxification and metabolic purposes
-
-
-
additional information
?
-
the physiological direction of the catalytic reaction is reduction rather than oxidation
-
-
-
additional information
?
-
the enzyme displays a preference for the reduction of alicyclic, bicyclic and aromatic ketones and alpha-ketoesters, but is poorly active on aliphatic, cyclic and aromatic alcohols, showing no activity on aldehydes
-
-
-
additional information
?
-
-
the enzyme displays a preference for the reduction of alicyclic, bicyclic and aromatic ketones and alpha-ketoesters, but is poorly active on aliphatic, cyclic and aromatic alcohols, showing no activity on aldehydes
-
-
-
additional information
?
-
the enzyme shows no activity on aliphatic linear and branched alcohols, except for a poor activity on 2-propyn-1-ol, 3-methyl-1-butanol and 2-pentanol; however, it shows a discrete activity on aliphatic cyclic and bicyclic alcohols. Benzyl alcohol and 4-bromobenzyl alcohol are not found to be substrates. The S and R enantiomers of a-(trifluoromethyl)benzyl alcohol and methyl and ethyl mandelates show no apparent activity with SaADH. The enzyme shows poor activity on (+/-)-1-phenyl-1-propanol, 1-(1-naphthyl)ethanol and the two enantiomers of 1-(2-naphthyl)ethanol. The enzyme is not active on aliphatic and aromatic aldehydes, and on aliphatic linear, branched and cyclic ketones except for 3-methylcyclohexanone. Catalytic inactivity is observed with acetophenone and (S)-a-(trifluoromethyl)benzyl
-
-
-
additional information
?
-
enzyme displays a preference for the reduction of alicyclic, bicyclic and aromatic ketones and alpha-keto esters, but is poorly active on aliphatic, cyclic and aromatic alcohols, and shows no activity on aldehydes
-
-
-
additional information
?
-
the physiological direction of the catalytic reaction is reduction rather than oxidation
-
-
-
additional information
?
-
the enzyme shows no activity on aliphatic linear and branched alcohols, except for a poor activity on 2-propyn-1-ol, 3-methyl-1-butanol and 2-pentanol; however, it shows a discrete activity on aliphatic cyclic and bicyclic alcohols. Benzyl alcohol and 4-bromobenzyl alcohol are not found to be substrates. The S and R enantiomers of a-(trifluoromethyl)benzyl alcohol and methyl and ethyl mandelates show no apparent activity with SaADH. The enzyme shows poor activity on (+/-)-1-phenyl-1-propanol, 1-(1-naphthyl)ethanol and the two enantiomers of 1-(2-naphthyl)ethanol. The enzyme is not active on aliphatic and aromatic aldehydes, and on aliphatic linear, branched and cyclic ketones except for 3-methylcyclohexanone. Catalytic inactivity is observed with acetophenone and (S)-a-(trifluoromethyl)benzyl
-
-
-
additional information
?
-
the enzyme displays a preference for the reduction of alicyclic, bicyclic and aromatic ketones and alpha-ketoesters, but is poorly active on aliphatic, cyclic and aromatic alcohols, showing no activity on aldehydes
-
-
-
additional information
?
-
enzyme displays a preference for the reduction of alicyclic, bicyclic and aromatic ketones and alpha-keto esters, but is poorly active on aliphatic, cyclic and aromatic alcohols, and shows no activity on aldehydes
-
-
-
additional information
?
-
the enzyme undergoes a substantial conformational change in the apo-holo transition, accompanied by loop movements at the domain interface
-
-
-
additional information
?
-
-
ethanol dehydrogenase activity of Thermoanaerobium brockii is both NAD and NADP linked, reversible, and not inhibited by low levels of reaction products
-
-
-
additional information
?
-
enzyme reduces aldehydes to (R)-alcohols with more than 99.8% enantiomeric excess
-
-
-
additional information
?
-
-
enzyme shows high substrate specificity towards primary aliphatic alcohols, no activity with 2-butanol, tert-butanol, isoamyl alcohol, isobutyl alcohol, 1,6-hexadiol, and mono-, di-, and triethanolamine
-
-
-
additional information
?
-
TADH is a NAD(H)-dependent enzyme and shows a very broad substrate spectrum producing exclusively the (S)-enantiomer in high enantiomeric excess (more than 99%) during asymmetric reduction of ketones
-
-
-
additional information
?
-
ADH exhibits a clear preference for primary alcohols and corresponding aldehydes for aliphatic substrates, in the oxidative direction activity steeply increases with chain length until 1-propanol and then decreases slightly again with growing chain length, alpha,beta-unsaturated ketones like 3-penten-2-one and cyclohexenone are not converted by ADH, almost no conversion of methanol (0.2%) and (+)-carvone (0.4%) is detected
-
-
-
additional information
?
-
TADH is a NAD(H)-dependent enzyme and shows a very broad substrate spectrum producing exclusively the (S)-enantiomer in high enantiomeric excess (more than 99%) during asymmetric reduction of ketones
-
-
-
additional information
?
-
ADH exhibits a clear preference for primary alcohols and corresponding aldehydes for aliphatic substrates, in the oxidative direction activity steeply increases with chain length until 1-propanol and then decreases slightly again with growing chain length, alpha,beta-unsaturated ketones like 3-penten-2-one and cyclohexenone are not converted by ADH, almost no conversion of methanol (0.2%) and (+)-carvone (0.4%) is detected
-
-
-
additional information
?
-
-
preference for reduction of aromatic ketones and alpha-keto esters, and poor activity on aromatic alcohols and aldehydes
-
-
-
additional information
?
-
-
the enzyme transfers the pro-S hydrogen of [4R-(2)H]NADH and exhibits Prelog specificity
-
-
-
additional information
?
-
-
the enzyme transfers the pro-S hydrogen of [4R-(2)H]NADH and exhibits Prelog specificity
-
-
-
additional information
?
-
enzyme selectively reduces the C=O bond of allylic aldehydes/ketones to the corresponding alpha,beta-unsaturated alcohols and also has the capacity of stereoselectively reducing aromatic ketones to (S)-enantioselective alcohols. The enzyme preferentially catalyzes oxidation of allylic/benzyl aldehydes
-
-
-
additional information
?
-
enzyme selectively reduces the C=O bond of allylic aldehydes/ketones to the corresponding alpha,beta-unsaturated alcohols and also has the capacity of stereoselectively reducing aromatic ketones to (S)-enantioselective alcohols. The enzyme preferentially catalyzes oxidation of allylic/benzyl aldehydes
-
-
-
additional information
?
-
-
key enzyme in ethanol production
-
-
-
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0.25
(+)-2-methylcyclohexanone
-
-
-
9.1
(2E)-but-2-en-1-ol
pH 8.0, 55°C
-
3.3
(2E)-but-2-enal
pH 6.5, 65°C
2.4
(R)-1-indanol
65°C, pH 8.0; pH 8.0, 65°C
0.41 - 37.4
(R)-2-butanol
0.22 - 31.7
(R)-2-pentanol
0.02
(R)-2-phenylpropanol
-
-
1.28 - 1.3
(R)-alpha-tetralol
18.1
(S)-(-)-1-phenylethanol
-
65°C
18.1
(S)-1-phenylethanol
-
pH 10.5, 65°C
0.012 - 120
(S)-2-butanol
0.07 - 8.8
(S)-2-pentanol
0.157
(S)-2-phenylpropanol
-
-
1.05 - 4.2
(S)-alpha-tetralol
8.47
(S)-N-benzyl-3-pyrrolidinol
-
pH 8.0
1.65 - 15.84
1,2-propanediol
26.7
1,3-Propanediol
-
pH 9.0, 60°C, recombinant enzyme
0.000036 - 0.0038
1-formyl-6-methylpyrene
0.000035 - 0.00149
1-formyl-8-methylpyrene
0.0005 - 0.012
1-formylpyrene
0.00028 - 0.0064
1-hydroxymethyl-6-methylpyrene
0.00031 - 0.059
1-hydroxymethyl-8-methylpyrene
0.00059 - 0.076
1-hydroxymethylpyrene
27.6
1-Indanone
-
65°C; pH 10.5, 65°C
5.9
1-phenyl-1,2-propandione
-
pH 10.5, 65°C
5 - 12.1
1-phenyl-1,2-propanedione
0.025 - 244
1-phenylethanol
0.2
11-cis-retinal
-
reduction with NADH
0.018 - 0.28
11-cis-retinol
0.013 - 2.3
12-hydroxydodecanoate
0.056
12-Hydroxydodecanoic acid
-
-
0.086 - 0.1
12-hydroxylauric acid methyl ester
-
0.066
12-oxolauric acid methyl ester
-
wild-type, pH 8.0, 60°C
-
0.11 - 0.137
13-cis-retinal
0.257
13-cis-retinol
-
oxidation with NAD+
0.06
16-hydroxyhexadecanoate
-
-
11.9
2',3',4',5',6'-pentafluoroacetophenone
-
65°C, pH 5.0
6.3 - 23.5
2,2,2-trifluoroacetophenone
0.11
2,2-dichloroacetophenone
-
65°C, pH 5.0
61.3
2,3-Butanediol
pH 9.0, 70°C
0.147
2-decanone
-
pH 8.0, 60°C
0.25
2-ethoxyethanol
-
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.0021 - 0.009
2-formylpyrene
1.16
2-Heptanone
-
pH 8.0, 60°C
5.01
2-Hexanone
-
pH 8.0, 60°C
0.00048 - 0.106
2-hydroxymethylpyrene
0.021
2-Methylcyclohexanol
-
-
2.7
2-methylpropan-1-ol
-
-
0.215
2-nonanone
-
pH 8.0, 60°C
0.286
2-Octanone
-
pH 8.0, 60°C
0.752
2-Pentanol
-
pH 8.0, 60°C
0.864
2-phenylpropionaldehyde
-
-
0.025 - 0.026
3,4-dihydro-retinal
0.024 - 0.028
3,4-dihydro-retinol
0.14 - 0.9
3-bromobenzyl alcohol
4.4
3-Methoxybenzaldehyde
-
65°C
1.2 - 3.3
3-Methoxybenzyl alcohol
7.5
3-methylbutan-2-one
-
-
9.9
3-Methylcyclohexanol
65°C, pH 8.0
66
3-methylcyclohexanone
65°C, pH 5.5
0.031
3-oxo-5beta-androstan-17beta-ol
-
-
0.032
3-Phenyl-1-propanol
-
-
0.033
3-phenylpropanol
-
-
0.128 - 0.31
3beta,12alpha-dihydroxy-5beta-cholanoate
0.149 - 0.182
3beta,7alpha,12alpha-trihydroxy-5beta-cholanoate
0.35
3beta,7alpha-12alpha-trihydroxy-5beta-cholanoate
-
isoenzyme 4
0.032 - 0.071
3beta-7alpha-dihydroxy-5beta-cholanoate
0.12 - 0.4
4-bromobenzyl alcohol
0.28
4-carboxybenzaldehyde
0.14 - 1.11
4-chloroacetophenone
0.00048 - 0.0038
4-formylpyrene
0.004 - 0.015
4-hydroxy-retinol
0.00037 - 0.04
4-hydroxymethylpyrene
0.024 - 4.4
4-methoxybenzaldehyde
0.07 - 61
4-methoxybenzyl alcohol
1.3
4-methoxybenzylalcohol
-
in the presence of 3 mM NAD+, in 0.1 M glycine-NaOH, pH 9.2, at 65°C
0.131
4-methoxyphenylacetone
-
pH 8.0, 60°C
-
6.7 - 53.8
4-Methyl-1-pentanol
0.81
4-methylpentan-1-ol
-
-
0.017 - 0.027
4-oxo-retinal
0.0036 - 0.04
5alpha-androstan-17beta-ol-3-one
0.011 - 0.046
5beta-androstan-17beta-ol-3-one
0.123 - 0.164
5beta-androstan-3beta-ol-17 one
0.003 - 0.058
5beta-androstan-3beta-ol-17-one
0.008 - 0.25
5beta-cholanic acid-3-one
0.047
5beta-Pregnan-21-ol-3,20-dione hemisuccinate
-
-
0.0036
5beta-pregnan-3,20-dione
-
pH 7.3, 37°C, ADH1C*2 (gamma2gamma2)
0.027
5beta-Pregnan-3beta-ol-20-one
-
pH 7.3, 37°C, ADH1C*2 (gamma2gamma2)
0.16 - 0.242
9-cis-retinal
0.011 - 0.3
9-cis-retinol
0.0086 - 121
acetaldehyde
0.011 - 0.34
all-trans-retinal
0.009 - 0.059
all-trans-retinol
0.39
allyl alcohol
-
pH 7.3, 37°C
5.3
alpha-tetralol
-
65°C
5.8
alpha-tetralone
-
65°C; pH 10.5, 65°C
0.0025 - 0.09
Anisaldehyde
0.43
benzil
-
65°C, pH 5.0
0.007 - 49
benzyl alcohol
0.0145 - 0.8
benzylalcohol
86.8
Butan-2,3-diol
-
pH 8.8, 70°C
0.06 - 0.27
Butyraldehyde
0.14 - 0.25
Capronaldehyde
0.03
cinnamaldehyde
-
pH 7.7, 60°C
0.11 - 4.58
cinnamyl alcohol
1
cis-4-methylcyclohexanol
-
-
4.2
cis-decahydro-1-naphthol
65°C, pH 8.0
0.71
crotyl alcohol
-
pH 7.3, 37°C
0.008 - 1900
Cyclohexanol
1.39 - 14.1
cyclohexanone
16.6
ethyl 3-methyl-2-oxobutyrate
-
65°C, pH 5.0
1 - 5.3
ethyl benzoylformate
5.3
ethyl pyruvate
-
pH 6.5, 50°C, recombinant enzyme
0.71
isatin
65°C, pH 5.5; pH 5.5, 65°C
2.4
iso-propanol
2 mM zinc sulfate in 100 mM glycine-NaOH (pH 10.5) at 65°C
0.81
isoborneol
-
65°C, pH 10.5
0.26 - 0.76
Isobutyraldehyde
197
Isopentanol
at pH 7.0 and 25°C
1.4
m-nitrobenzaldehyde
-
isoenzyme ADH-1, pH 7.5
6.5
methyl benzoylformate
65°C, pH 5.5
2.8
methyl o-chlorobenzoylformate
65°C, pH 5.5; pH 5.5, 65°C
2.7
methyl oxo(phenyl)acetate
0.13 - 0.58
N-benzyl-3-pyrrolidinone
10.3
n-Pentanal
-
33°C, pH 8
0.011
octan-1-ol
-
enzyme form ADH-3
2.9 - 5.9
p-Methoxybenzaldehyde
6.9 - 22
p-methoxybenzyl alcohol
13.6
phenyl trifluoromethyl ketone
-
25°C, pH 7.0
0.19 - 0.91
phenylacetaldehyde
24.45 - 37.55
phenylglyoxylic acid
0.59
propanal
-
33°C, pH 7
2.01
propanone
-
33°C, pH 7
3.4
R-(+)-1-phenylethanol
-
-
1.2
R-(+)-cis-verbenol
-
-
1.5
R-(+)-trans-bicyclo(2.2.1)-heptanol
-
-
3.9
S-(-)-1-phenylethanol
-
-
0.95
S-(-)-2-methylbutan-1-ol
-
-
3.8
S-(-)-cis-bicyclo(2.2.1)-heptanol
-
-
0.85
S-(-)-trans-bicyclo(2.2.1)-heptanol
-
-
0.694
tert-butyl acetoacetate
-
pH 8.0, 60°C
-
8.5
tetralin-1-ol
-
65°C, pH 10.5
1.1
trans-4-methylcyclohexanol
-
-
0.009 - 0.23
trans-cinnamaldehyde
0.034 - 11
Vanillyl alcohol
additional information
additional information
-
0.41
(R)-2-butanol

-
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
37.4
(R)-2-butanol
-
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.22
(R)-2-pentanol

-
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
11.7
(R)-2-pentanol
-
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
1.28
(R)-alpha-tetralol

pH 8.0, 65°C
1.3
(R)-alpha-tetralol
65°C, pH 8.0
4.2
(S)-(+)-1-indanol

-
65°C
4.2
(S)-(+)-alpha-tetraol
-
65°C
4.2
(S)-1-indanol

-
pH 10.5, 65°C
6.4
(S)-1-indanol
65°C, pH 8.0; pH 8.0, 65°C
0.012
(S)-2-butanol

-
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
29.8
(S)-2-butanol
-
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.07
(S)-2-pentanol

-
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
8.8
(S)-2-pentanol
-
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
1.05
(S)-alpha-tetralol

65°C, pH 8.0; pH 8.0, 65°C
4.2
(S)-alpha-tetralol
-
pH 10.5, 65°C
1.65
1,2-propanediol

-
-
11.5
1,2-propanediol
-
pH 8.0, 60°C, recombinant enzyme
15.84
1,2-propanediol
-
-
0.08
1-butanol

-
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.17
1-butanol
-
isoenzyme ADH-3, pH 10.0
0.596
1-butanol
-
pH 8.0, 60°C
1.6
1-butanol
-
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
17
1-butanol
-
isoenzyme ADH-1, pH 10.0
230
1-butanol
-
isoenzyme ADH-1, pH 7.5
0.000036
1-formyl-6-methylpyrene

isozyme ADH4, at 21-23°C
0.00032
1-formyl-6-methylpyrene
isozyme ADH2, at 21-23°C
0.0009
1-formyl-6-methylpyrene
isozyme ADH1C, at 21-23°C
0.0038
1-formyl-6-methylpyrene
isozyme ADH3, at 21-23°C
0.000035
1-formyl-8-methylpyrene

isozyme ADH4, at 21-23°C
0.00024
1-formyl-8-methylpyrene
isozyme ADH2, at 21-23°C
0.00131
1-formyl-8-methylpyrene
isozyme ADH1C, at 21-23°C
0.00149
1-formyl-8-methylpyrene
isozyme ADH3, at 21-23°C
0.0005
1-formylpyrene

isozyme ADH1C, at 21-23°C
0.00055
1-formylpyrene
isozyme ADH3, at 21-23°C
0.00092
1-formylpyrene
isozyme ADH4, at 21-23°C
0.012
1-formylpyrene
isozyme ADH2, at 21-23°C
0.038
1-Heptanol

-
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.21
1-Heptanol
-
mutant enzyme W95L, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.67
1-Heptanol
-
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.035
1-Hexanol

-
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.04
1-Hexanol
-
mutant enzyme W95L, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.147
1-Hexanol
-
pH 8.0, 60°C
0.88
1-Hexanol
-
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.00028
1-hydroxymethyl-6-methylpyrene

isozyme ADH4, at 21-23°C
0.00057
1-hydroxymethyl-6-methylpyrene
isozyme ADH3, at 21-23°C
0.00115
1-hydroxymethyl-6-methylpyrene
isozyme ADH1C, at 21-23°C
0.0064
1-hydroxymethyl-6-methylpyrene
isozyme ADH2, at 21-23°C
0.00031
1-hydroxymethyl-8-methylpyrene

isozyme ADH3, at 21-23°C
0.0016
1-hydroxymethyl-8-methylpyrene
isozyme ADH4, at 21-23°C
0.0064
1-hydroxymethyl-8-methylpyrene
isozyme ADH2, at 21-23°C
0.059
1-hydroxymethyl-8-methylpyrene
isozyme ADH1C, at 21-23°C
0.00059
1-hydroxymethylpyrene

isozyme ADH3, at 21-23°C
0.00075
1-hydroxymethylpyrene
isozyme ADH1C, at 21-23°C
0.0283
1-hydroxymethylpyrene
isozyme ADH4, at 21-23°C
0.076
1-hydroxymethylpyrene
isozyme ADH2, at 21-23°C
5.1
1-Indanol

-
65°C
8.7
1-Indanol
-
65°C, pH 10.5
0.025
1-Octanol

-
isoenzyme ADH-3, pH 10.0
0.1
1-Octanol
-
isoenzyme ADH-2
0.39
1-Octanol
isozyme ADH3, at 21-23°C
0.5
1-Octanol
-
isoenzyme ADH-1, pH 7.5
0.51
1-Octanol
-
isoenzyme ADH-2, pH 10.0
3.88
1-Octanol
cosubstrate NADP+, pH 8.0, 60°C
0.027
1-Pentanol

-
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.08
1-Pentanol
-
isoenzyme ADH-3, pH 10.0
0.26
1-Pentanol
-
mutant enzyme W95L, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.396
1-Pentanol
-
pH 8.0, 60°C
1.9
1-Pentanol
-
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
3.1
1-Pentanol
-
isoenzyme ADH-1, pH 10.0
78
1-Pentanol
-
isoenzyme ADH-2, pH 10.0
5
1-phenyl-1,2-propanedione

-
65°C, pH 5.0
5.9
1-phenyl-1,2-propanedione
-
65°C
12.1
1-phenyl-1,2-propanedione
65°C, pH 5.5
0.025
1-phenylethanol

-
-
244
1-phenylethanol
pH 9.0, 70°C
0.24
1-propanol

-
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.33
1-propanol
-
native enzyme, at 55°C
0.33
1-propanol
-
in the presence of 3 mM NAD+, in 0.1 M glycine-NaOH, pH 9.2, at 65°C
0.6
1-propanol
-
wild type enzyme, at 65°C
1.03
1-propanol
-
pH 8.0, 60°C
1.7
1-propanol
-
mutant enzyme N249Y, at 65°C
2.4
1-propanol
-
carboxymethylated enzyme, at 55°C
6.5
1-propanol
-
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.018
11-cis-retinol

-
isozyme ADH1B2, pH 7.5, 25°C
0.035
11-cis-retinol
-
isozyme ADH1B1, pH 7.5, 25°C
0.28
11-cis-retinol
-
oxidation with NAD+
0.013
12-hydroxydodecanoate

-
isoenzyme ADH-3, pH 10.0
0.02
12-hydroxydodecanoate
-
isoenzyme BB-ADH
0.04
12-hydroxydodecanoate
-
enzyme form ADH-3
0.047
12-hydroxydodecanoate
-
-
0.048
12-hydroxydodecanoate
-
-
0.05
12-hydroxydodecanoate
-
isoenzyme BB-ADH and isoenzyme ADH-TT
0.06
12-hydroxydodecanoate
-
isoenzyme AA-ADH
0.1
12-hydroxydodecanoate
-
-
0.1
12-hydroxydodecanoate
-
isoenzyme ADH-1, pH 10.0
0.23
12-hydroxydodecanoate
-
-
1.4
12-hydroxydodecanoate
-
isoenzyme ADH-1, pH 10.0
2.3
12-hydroxydodecanoate
-
isoenzyme TT-ADH
0.086
12-hydroxylauric acid methyl ester

-
wild-type, pH 8.0, 60°C
-
0.1
12-hydroxylauric acid methyl ester
-
mutant C257L, pH 8.0, 60°C
-
0.11
13-cis-retinal

-
oxidation with NAD+
0.137
13-cis-retinal
-
reduction with NADH
6.3
2,2,2-trifluoroacetophenone

-
65°C, pH 5.0
11.2
2,2,2-trifluoroacetophenone
-
65°C; pH 10.5, 65°C
23.5
2,2,2-trifluoroacetophenone
65°C, pH 5.5
1.05
2-butanol

-
pH 8.0, 60°C
11.9
2-butanol
-
33°C, pH 8
0.35
2-Buten-1-ol

-
isoenzyme ADH-3, pH 10.0
2.5
2-Buten-1-ol
-
isoenzyme ADH-1, pH 10.0
60
2-Buten-1-ol
-
isoenzyme ADH-2, pH 10.0
0.0021
2-formylpyrene

isozyme ADH1C, at 21-23°C
0.0029
2-formylpyrene
isozyme ADH2, at 21-23°C
0.0069
2-formylpyrene
isozyme ADH4, at 21-23°C
0.009
2-formylpyrene
isozyme ADH3, at 21-23°C
0.00048
2-hydroxymethylpyrene

isozyme ADH1C, at 21-23°C
0.0044
2-hydroxymethylpyrene
isozyme ADH2, at 21-23°C
0.033
2-hydroxymethylpyrene
isozyme ADH4, at 21-23°C
0.106
2-hydroxymethylpyrene
isozyme ADH3, at 21-23°C
5.15
2-Pentanone

-
pH 8.0, 60°C
0.6
2-propanol

-
native enzyme, at 55°C
0.6
2-propanol
-
in the presence of 3 mM NAD+, in 0.1 M glycine-NaOH, pH 9.2, at 65°C
2.44
2-propanol
-
pH 8.0, 60°C
19.6
2-propanol
-
33°C, pH 8
53
2-propanol
-
carboxymethylated enzyme, at 55°C
57.5
2-propanol
-
25°C, pH 7.0
57.8
2-propanol
-
pH 7.7, 60°C
268
2-propanol
-
wilde-type enzyme ADh1
0.025
3,4-dihydro-retinal

-
pH 7.5, 25°C, isozyme ADH1B2
0.026
3,4-dihydro-retinal
-
pH 7.5, 25°C, isozyme ADH4
0.024
3,4-dihydro-retinol

-
pH 7.5, 25°C, isozyme ADH1B2
0.028
3,4-dihydro-retinol
-
pH 7.5, 25°C, isozyme ADH4
0.14
3-bromobenzyl alcohol

-
native enzyme, at 55°C
0.9
3-bromobenzyl alcohol
-
carboxymethylated enzyme, at 55°C
0.14
3-bromobenzylalcohol
-
in the presence of 3 mM NAD+, in 0.1 M glycine-NaOH, pH 9.2, at 65°C
1.2
3-Methoxybenzyl alcohol

-
native enzyme, at 55°C
3.3
3-Methoxybenzyl alcohol
-
carboxymethylated enzyme, at 55°C
1.2
3-methoxybenzylalcohol
-
in the presence of 3 mM NAD+, in 0.1 M glycine-NaOH, pH 9.2, at 65°C; in the presence of 5 mM benzylalcohol, in 0.1 M glycine-NaOH, pH 9.2, at 65°C
0.27
3-Pentanol

-
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.128
3beta,12alpha-dihydroxy-5beta-cholanoate

-
isoenzyme 2
0.16
3beta,12alpha-dihydroxy-5beta-cholanoate
-
isoenzyme 3
0.248
3beta,12alpha-dihydroxy-5beta-cholanoate
-
isoenzyme 4
0.31
3beta,12alpha-dihydroxy-5beta-cholanoate
-
isoenzyme I
0.149
3beta,7alpha,12alpha-trihydroxy-5beta-cholanoate

-
isoenzyme I
0.16
3beta,7alpha,12alpha-trihydroxy-5beta-cholanoate
-
isoenzyme 2
0.182
3beta,7alpha,12alpha-trihydroxy-5beta-cholanoate
-
isoenzyme 3
0.032
3beta-7alpha-dihydroxy-5beta-cholanoate

-
isoenzyme 3
0.064
3beta-7alpha-dihydroxy-5beta-cholanoate
-
isoenzyme 2
0.066
3beta-7alpha-dihydroxy-5beta-cholanoate
-
isoenzyme 1
0.071
3beta-7alpha-dihydroxy-5beta-cholanoate
-
isoenzyme 4
0.12
4-bromobenzyl alcohol

-
native enzyme, at 55°C
0.4
4-bromobenzyl alcohol
-
carboxymethylated enzyme, at 55°C
0.12
4-bromobenzylalcohol
-
in the presence of 3 mM NAD+, in 0.1 M glycine-NaOH, pH 9.2, at 65°C
0.28
4-carboxybenzaldehyde

-
native enzyme, at 55°C
0.28
4-carboxybenzaldehyde
-
in 50 mM Tris-HCl (pH 7.5) containing 0.2 mM NADH + H+, at 65°C
0.14
4-chloroacetophenone

-
pH 6.0, 37°C, recombinant enzyme expressed from Hansenula polymorpha
0.53
4-chloroacetophenone
-
pH 6.0, 37°C, recombinant enzyme expressed from Saccharomyces cerevisiae
1.11
4-chloroacetophenone
-
pH 6.0, 37°C, recombinant enzyme expressed from Arxula adeninivorans
0.00048
4-formylpyrene

isozyme ADH4, at 21-23°C
0.001
4-formylpyrene
isozyme ADH1C, at 21-23°C
0.0021
4-formylpyrene
isozyme ADH3, at 21-23°C
0.0038
4-formylpyrene
isozyme ADH2, at 21-23°C
0.004
4-hydroxy-retinol

-
pH 7.5, 25°C, isozyme ADH1B1
0.011
4-hydroxy-retinol
-
pH 7.5, 25°C, isozyme ADH1B2
0.015
4-hydroxy-retinol
-
pH 7.5, 25°C, isozyme ADH4
0.00037
4-hydroxymethylpyrene

isozyme ADH3, at 21-23°C
0.001
4-hydroxymethylpyrene
isozyme ADH1C, at 21-23°C
0.0029
4-hydroxymethylpyrene
isozyme ADH4, at 21-23°C
0.04
4-hydroxymethylpyrene
isozyme ADH2, at 21-23°C
0.024
4-methoxybenzaldehyde

-
wild type enzyme, at 65°C
0.14
4-methoxybenzaldehyde
-
native enzyme, at 55°C
0.14
4-methoxybenzaldehyde
-
in 50 mM Tris-HCl (pH 7.5) containing 0.2 mM NADH + H+, at 65°C
0.86
4-methoxybenzaldehyde
-
mutant enzyme N249Y, at 65°C
4.4
4-methoxybenzaldehyde
-
pH 6.0, 65°C
0.07
4-methoxybenzyl alcohol

-
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.12
4-methoxybenzyl alcohol
-
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.23
4-methoxybenzyl alcohol
-
wild type enzyme, at 65°C
1.13
4-methoxybenzyl alcohol
-
pH 8.0, 60°C
1.24
4-methoxybenzyl alcohol
-
mutant enzyme N249Y, at 65°C
1.3
4-methoxybenzyl alcohol
-
native enzyme, at 55°C
1.3
4-methoxybenzyl alcohol
-
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
1.4
4-methoxybenzyl alcohol
-
carboxymethylated enzyme, at 55°C
61
4-methoxybenzyl alcohol
-
65°C; pH 10.5, 65°C
6.7
4-Methyl-1-pentanol

-
mutant enzyme W54L
53.8
4-Methyl-1-pentanol
-
wilde-type enzyme ADh1
0.017
4-oxo-retinal

-
pH 7.5, 25°C, isozyme ADH4
0.025
4-oxo-retinal
-
pH 7.5, 25°C, isozyme ADH1B1
0.027
4-oxo-retinal
-
pH 7.5, 25°C, isozyme ADH1B2
0.0036
5alpha-androstan-17beta-ol-3-one

-
pH 7.3, 37°C, ADH1C*2 (gamma2gamma2)
0.021
5alpha-androstan-17beta-ol-3-one
-
isoenzyme 2
0.025
5alpha-androstan-17beta-ol-3-one
-
isoenzyme 2
0.027
5alpha-androstan-17beta-ol-3-one
-
isoenzyme 4
0.04
5alpha-androstan-17beta-ol-3-one
-
isoenzyme 1
0.011
5beta-androstan-17beta-ol-3-one

-
pH 7.3, 37°C, recombinant enzyme
0.046
5beta-androstan-17beta-ol-3-one
-
pH 7.3, 37°C, ADH1C*2 (gamma2gamma2)
0.123
5beta-androstan-3beta-ol-17 one

-
isoenzyme 4
0.125
5beta-androstan-3beta-ol-17 one
-
isoenzyme 2
0.14
5beta-androstan-3beta-ol-17 one
-
isoenzyme 3
0.164
5beta-androstan-3beta-ol-17 one
-
isoenzyme 1
0.003
5beta-androstan-3beta-ol-17-one

-
pH 7.3, 37°C, recombinant enzyme
0.058
5beta-androstan-3beta-ol-17-one
-
pH 7.3, 37°C, ADH1C*2 (gamma2gamma2)
0.008
5beta-cholanic acid-3-one

-
pH 7.3, 37°C, recombinant enzyme
0.25
5beta-cholanic acid-3-one
-
pH 7.3, 37°C, ADH1C*2 (gamma2gamma2)
0.16
9-cis-retinal

-
oxidation with NAD+
0.242
9-cis-retinal
-
reduction with NADH
0.011
9-cis-retinol

-
isozyme ADH1B1, pH 7.5, 25°C
0.023
9-cis-retinol
-
isozyme ADH1B2, pH 7.5, 25°C
0.3
9-cis-retinol
-
oxidation with NAD+
0.0086
acetaldehyde

Sporotrichum pulverulentum
-
-
0.013
acetaldehyde
-
70°C, pH 9.0, 50 mM Tris-HCl, 4 M NaCl
0.059
acetaldehyde
-
40°C, pH 9.0, 50 mM Tris-HCl, 4 M NaCl
0.085
acetaldehyde
-
isoenzyme beta1,beta1
0.086
acetaldehyde
-
enzyme form ADH I
0.1
acetaldehyde
-
pyrazole-sensitive enzyme, pH 7.0
0.217
acetaldehyde
-
isoenzyme 3
0.23
acetaldehyde
-
isoenzyme 2
0.24
acetaldehyde
-
isoenzyme gamma2,gamma2; isoenzymes beta2,beta2
0.24
acetaldehyde
-
isoenzymes beta2,beta2
0.276
acetaldehyde
-
isoenzyme 4
0.3
acetaldehyde
-
isozyme ADH I, pH 5.5, 25°C
0.31
acetaldehyde
-
isoenzyme 1
0.33
acetaldehyde
-
isoenzyme gamma1,gamma1
0.34
acetaldehyde
isozyme ADH1C, at 21-23°C
0.364
acetaldehyde
-
wild-type, pH 8.0, 60°C
0.67
acetaldehyde
pH 6.0, 22°C, recombinant enzyme
0.92
acetaldehyde
-
33°C, pH 7
1.2
acetaldehyde
pH 7.0, temperature not specified in the publication
1.25
acetaldehyde
-
isoenzyme II
1.3
acetaldehyde
-
enzyme form ADH II
1.45
acetaldehyde
-
isoenzyme I
1.9
acetaldehyde
-
pH 8.5
2.25
acetaldehyde
cosubstrate NADPH, pH 8.0, 60°C
2.6
acetaldehyde
-
pH 8.0, 60°C, recombinant enzyme
3.4
acetaldehyde
-
isoenzyme beta3,beta3
4
acetaldehyde
pH 7.0, temperature not specified in the publication
4.3
acetaldehyde
-
isoenzyme alpha,alpha
4.71
acetaldehyde
cosubstrate NADH, pH 8.0, 60°C
11.5
acetaldehyde
-
pyrazole-insensitive enzyme, pH 7.0
12.7
acetaldehyde
isozyme ADH4, at 21-23°C
24
acetaldehyde
-
isozyme ADH II, pH 6.0, 25°C
26
acetaldehyde
isozyme ADH2, at 21-23°C
28
acetaldehyde
pH 6.0, temperature not specified in the publication
34
acetaldehyde
wild-type, 60°C, pH 6.0, presence of 0.1 mM Zn2+
70
acetaldehyde
-
mutant enzyme ADH1-1S1108
85
acetaldehyde
-
wild-type enzyme ADH1-1S
121
acetaldehyde
alcohol dehydrogenase domain, 60°C, pH 6.0, presence of 0.1 mM Zn2+
6.5
acetoin

-
reduction reaction, pH 6.1, 70°C
30.2
acetoin
pH 6.0, 70°C
6.6
acetone

-
-
0.2
acetophenone

-
pH 6.0, 37°C, recombinant enzyme expressed from Hansenula polymorpha
0.4
acetophenone
-
pH 6.0, 37°C, recombinant enzyme expressed from Arxula adeninivorans
0.66
acetophenone
-
pH 6.0, 37°C, recombinant enzyme expressed from Saccharomyces cerevisiae
0.011
all-trans-retinal

-
pH 7.5, 25°C, isozyme ADH1B1
0.012
all-trans-retinal
-
pH 7.5, 25°C, isozyme ADH1B2
0.034
all-trans-retinal
-
pH 7.5, 25°C, isozyme ADH4
0.26
all-trans-retinal
-
oxidation with NAD+
0.34
all-trans-retinal
-
reduction with NADH
0.009
all-trans-retinol

-
-
0.02
all-trans-retinol
-
isoenzyme TT-ADH
0.023
all-trans-retinol
-
pH 7.5, 25°C, isozyme ADH4
0.03
all-trans-retinol
-
isoenzyme BB-ADH
0.03
all-trans-retinol
-
pH 7.5, 25°C, isozyme ADH1B1
0.03
all-trans-retinol
-
isozyme ADH1B1, pH 7.5, 25°C
0.033
all-trans-retinol
-
pH 7.5, 25°C, isozyme ADH1B2
0.033
all-trans-retinol
-
isozyme ADH1B2, pH 7.5, 25°C
0.05
all-trans-retinol
-
isoenzyme AA-ADH
0.059
all-trans-retinol
-
oxidation with NAD+
0.0025
Anisaldehyde

-
-
0.03
benzaldehyde

-
wild type enzyme, at 65°C
0.03
benzaldehyde
-
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.07
benzaldehyde
-
mutant enzyme W95L, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.2
benzaldehyde
-
carboxymethylated enzyme, at 55°C
0.26
benzaldehyde
-
mutant enzyme N249Y, at 65°C
0.28
benzaldehyde
-
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.3
benzaldehyde
-
native enzyme, at 55°C
0.3
benzaldehyde
-
in 50 mM Tris-HCl (pH 7.5) containing 0.2 mM NADH + H+, at 65°C
0.333
benzaldehyde
-
pH 8.0, 60°C
0.4
benzaldehyde
pH 6.5, 65°C
2 - 3
benzaldehyde
in the presence of 0.25 mM NADH, in 83 mM potassium phosphate, 40 mM KCl, and 0.25 mM EDTA buffer, pH 7.3, at 30°C
14
benzaldehyde
-
isozyme ADH2, in the presence of 0.25 mM NADH, in 83 mM potassium phosphate, 40 mM KCl, and 0.25 mM EDTA buffer, pH 7.3, at 30°C
33
benzaldehyde
-
isozyme ADH1, in the presence of 0.25 mM NADH, in 83 mM potassium phosphate, 40 mM KCl, and 0.25 mM EDTA buffer, pH 7.3, at 30°C
0.007
benzyl alcohol

-
-
0.013
benzyl alcohol
-
enzyme form ADH-2
0.05
benzyl alcohol
-
isoenzyme ADH-3, pH 10.0
0.12
benzyl alcohol
-
mutant enzyme W95L, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.26
benzyl alcohol
-
wild type enzyme, at 65°C
0.26
benzyl alcohol
-
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.8
benzyl alcohol
-
native enzyme, at 55°C
0.8
benzyl alcohol
-
mutant Y25A/W49F/W167Y, 30°C, pH not specified in the publication
1.4
benzyl alcohol
-
isoenzyme ADH-1, pH 10.0
1.5
benzyl alcohol
-
mutant enzyme N249Y, at 65°C
1.5
benzyl alcohol
-
mutant W87A, pH 7.0, 30°C
2
benzyl alcohol
-
carboxymethylated enzyme, at 55°C
3.1
benzyl alcohol
-
mutant W49F/W167Y, 30°C, pH not specified in the publication
3.8
benzyl alcohol
-
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
4.2
benzyl alcohol
-
mutant V260A, 30°C, pH not specified in the publication
5.4
benzyl alcohol
-
mutant Y25A/W49F/W167Y/V260A, 30°C, pH not specified in the publication
5.43
benzyl alcohol
-
pH 8.0, 60°C
6
benzyl alcohol
-
mutant W49F/W87F, 30°C, pH not specified in the publication
6.8
benzyl alcohol
-
wild-type, 30°C, pH not specified in the publication
6.9
benzyl alcohol
-
wild-type, pH 7.0, 30°C
7.2
benzyl alcohol
-
mutant Y25A, pH 7.0, 30°C
8.2
benzyl alcohol
-
mutant W49F/W167Y/V260A, 30°C, pH not specified in the publication
10.1
benzyl alcohol
-
mutant Y25A/W49F/W87F/V260A, 30°C, pH not specified in the publication
10.9
benzyl alcohol
-
mutant Y25A/W49F/W87F, 30°C, pH not specified in the publication
12.9
benzyl alcohol
-
mutant W49F/W87F/V260A, 30°C, pH not specified in the publication
14
benzyl alcohol
pH 8.0, 55°C
16
benzyl alcohol
mutant A25Y, pH 7.0, 30°C
16.5
benzyl alcohol
-
mutant Y25A, 30°C, pH not specified in the publication
24
benzyl alcohol
in the presence of 2 mM NAD+, in 83 mM potassium phosphate, 40 mM KCl, and 0.25 mM EDTA buffer, pH 7.3, at 30°C
29.4
benzyl alcohol
wild-type, pH 7.0, 30°C
34
benzyl alcohol
-
isozyme ADH1, in the presence of 2 mM NAD+, in 83 mM potassium phosphate, 40 mM KCl, and 0.25 mM EDTA buffer, pH 7.3, at 30°C
49
benzyl alcohol
-
isozyme ADH2, in the presence of 2 mM NAD+, in 83 mM potassium phosphate, 40 mM KCl, and 0.25 mM EDTA buffer, pH 7.3, at 30°C
0.0145
benzylalcohol

in 50 mM glycine-NaOH at pH 10.0
0.44
benzylalcohol
2 mM zinc sulfate in 100 mM glycine-NaOH (pH 10.5) at 65°C
0.8
benzylalcohol
-
in the presence of 3 mM NAD+, in 0.1 M glycine-NaOH, pH 9.2, at 65°C
240
beta-NAD+

-
in presence of 0.5 mM SDS
243
beta-NAD+
-
in presence of 0.5 mM SDS
350
beta-NAD+
-
in presence of 1 mM SDS
710
beta-NAD+
-
in presence of 3 mM SDS
1230
beta-NAD+
-
in presence of 50 mM SDS
2290
beta-NAD+
-
in presence of 15 mM SDS
0.055
butan-1-ol

-
-
2
butan-1-ol
-
enzyme form ADH-3
0.35
butan-2-ol

-
-
93.3
butan-2-ol
-
wilde-type enzyme ADh1
3
Butanal

-
isoenzyme ADH-1, pH 7.5
4.24
Butanal
-
33°C, pH 7
0.012
butanol

-
pyrazole-sensitive enzyme, pH 7.5
0.02
butanol
-
pyrazole-sinensitive enzyme, pH 10.0
0.06
butanol
-
pyrazole-insensitive enzyme, pH 7.5
0.09
butanol
-
pyrazole-sensitive enzyme, pH 10.0
29.4
butanol
-
mutant enzyme W54L
53.9
butanol
-
wilde-type enzyme ADh1
0.06
Butyraldehyde

-
-
0.07
Butyraldehyde
-
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.108
Butyraldehyde
-
wild-type, pH 8.0, 60°C
0.13
Butyraldehyde
-
mutant enzyme W95L, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.27
Butyraldehyde
-
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.14
Capronaldehyde

-
pH 6.0, 37°C, recombinant enzyme expressed from Arxula adeninivorans
0.22
Capronaldehyde
-
pH 6.0, 37°C, recombinant enzyme expressed from Saccharomyces cerevisiae
0.25
Capronaldehyde
-
pH 6.0, 37°C, recombinant enzyme expressed from Hansenula polymorpha
0.11
cinnamyl alcohol

-
pH 7.7, 60°C
1.5
cinnamyl alcohol
-
mutant enzyme W54L
4.58
cinnamyl alcohol
-
wilde-type enzyme ADh1
5.1
cycloheptanol

-
65°C, pH 10.5
9.3
cycloheptanol
65°C, pH 8.0; pH 8.0, 65°C
0.008
Cyclohexanol

-
isoenzyme alpha,gamma1
0.03
Cyclohexanol
-
wild type enzyme, at 65°C
0.03
Cyclohexanol
-
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.04
Cyclohexanol
-
isoenzyme BB-ADH
0.045
Cyclohexanol
-
native enzyme, at 55°C
0.045
Cyclohexanol
-
in the presence of 3 mM NAD+, in 0.1 M glycine-NaOH, pH 9.2, at 65°C
0.23
Cyclohexanol
-
carboxymethylated enzyme, at 55°C
0.703
Cyclohexanol
-
pH 8.0, 60°C
0.76
Cyclohexanol
-
isoenzyme AA-ADH
1.9
Cyclohexanol
-
mutant enzyme N249Y, at 65°C
2.09
Cyclohexanol
in 200 mM bicine, pH 9.0, at 60°C
2.2
Cyclohexanol
-
isoenzyme ADH-3, pH 10.0
4.5
Cyclohexanol
-
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
5.2
Cyclohexanol
-
enzyme form ADH-3
23
Cyclohexanol
-
isoenzyme beta1,beta1
220
Cyclohexanol
-
isoenzyme ADH-1, pH 10.0
320
Cyclohexanol
-
isoenzyme TT-ADH
1900
Cyclohexanol
-
isoenzyme ADH-2, pH 10.0
1.39
cyclohexanone

-
pH 8.0, 60°C
3.68
cyclohexanone
in 200 mM bicine, pH 6.0, at 60°C
0.0172
ethanol

in 50 mM glycine-NaOH at pH 10.0
0.022
ethanol
-
isoenzyme beta1,beta1
0.022
ethanol
-
70°C, pH 9.0, 50 mM Tris-HCl, 4 M NaCl
0.049
ethanol
-
isoenzyme beta1,beta1
0.147
ethanol
20°C, pH 9.0
0.168
ethanol
30°C, pH 9.0
0.18
ethanol
-
pyrazole-sensitive enzyme, pH 7.5
0.19
ethanol
10°C, pH 9.0
0.248
ethanol
40°C, pH 9.0
0.34
ethanol
-
40°C, pH 9.0, 50 mM Tris-HCl, 4 M NaCl
0.43
ethanol
50°C, pH 9.0
0.45
ethanol
pH 9.0, 22°C, recombinant enzyme
0.48
ethanol
-
recombinant isozyme ADH1, pH 7.5, 25°C
0.5
ethanol
-
isoenzyme AA-ADH
0.63
ethanol
-
isoenzyme gamma2,gamma2
0.7
ethanol
-
native enzyme, at 55°C
0.7
ethanol
-
in the presence of 3 mM NAD+, in 0.1 M glycine-NaOH, pH 9.2, at 65°C
0.76
ethanol
-
isoenzyme 2
0.76
ethanol
-
isoenzyme BB-ADH
0.77
ethanol
isozyme ADH1C, at 21-23°C
0.8
ethanol
-
pyrazole-sensitive enzyme, pH 10.0
0.83
ethanol
-
recombinant isozyme ADH1, pH 10.5, 25°C
0.84
ethanol
-
isoenzyme beta2,beta2
0.91
ethanol
-
wild-type, pH 8.0, 60°C
0.94
ethanol
-
isoenzyme beta2,beta2
1
ethanol
-
isoenzyme gamma1,gamma1
1.04
ethanol
60°C, pH 9.0
1.33
ethanol
2 mM zinc sulfate in 100 mM glycine-NaOH (pH 10.5) at 65°C
1.37
ethanol
-
isoenzyme 1
1.4
ethanol
-
isoenzyme ADH-3, pH 10.0 and pH 7.5
1.41
ethanol
-
isoenzyme 4
1.5
ethanol
-
isoenzyme alpha,alpha
1.5
ethanol
-
mutant C257L, pH 8.0, 60°C
1.6
ethanol
-
isoenzyme beta1,beta1
1.7
ethanol
-
isoenzyme alpha,gamma1
1.7
ethanol
-
isoenzyme gamma2,gamma2
1.7
ethanol
-
isoenzyme III
1.87
ethanol
-
isoenzyme 3
2.3
ethanol
-
isozyme ADH I, pH 8.5, 25°C
2.46
ethanol
-
pH 7.3, 37°C
2.5
ethanol
Sporotrichum pulverulentum
-
-
3.1
ethanol
at pH 9.0 in bis-Tris propane buffer
3.2
ethanol
-
isoenzyme gamma1,gamma1
3.2
ethanol
at pH 7.0 and 25°C
3.5
ethanol
-
isoenzyme III from leaf
3.6
ethanol
isozyme ADH4, at 21-23°C
4
ethanol
-
pyrazole-insensitive enzyme, pH 7.5
4
ethanol
-
wild-type enzyme Adh1
4.2
ethanol
-
isoenzyme alpha,alpha
4.6
ethanol
-
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
4.8
ethanol
-
enzyme form ADH I
5
ethanol
-
pyrazole-insensitive enzyme, pH 10.0
5.7
ethanol
-
20°C, pH 9.0
5.7
ethanol
in 100 mM Tris-HCl, at pH 5.0 and 75°C
7.2
ethanol
-
isoenzyme II from leaf
7.3
ethanol
-
isozyme ADH II, pH 8.5, 25°C
7.55
ethanol
cosubstrate NAD+, pH 8.0, 60°C
7.75
ethanol
-
in 50 mM Tris-HCl, pH 8.0 at 25°C
8.1
ethanol
-
enzyme form ADH-3
8.56
ethanol
-
30°C, pH 9.0
9
ethanol
-
recombinant allozyme Ile308, pH 7.5, 25°C
9.03
ethanol
-
10°C, pH 9.0
10.4
ethanol
-
pH 9.0, 60°C, recombinant enzyme
10.6
ethanol
-
recombinant allozyme Val308, pH 7.5, 25°C
12
ethanol
-
pH 7.5, 25°C, Co-ADH
12.1
ethanol
-
40°C, pH 9.0
13
ethanol
-
in presence of 0.5 mM SDS
13.4
ethanol
-
mutant enzyme W54L
13.7
ethanol
-
pH 8.0, 60°C
14
ethanol
-
in presence of 3 mM SDS
17
ethanol
-
in presence of 1 mM SDS
17
ethanol
pH 7.0, temperature not specified in the publication
22
ethanol
-
mutant enzyme ADH1S-1108
24
ethanol
-
wild-type enzyme ADH1-1S
24.2
ethanol
-
pH 10.0, 50°C
26
ethanol
pH 7.0, temperature not specified in the publication
27
ethanol
-
enzyme form ADH II
27.5
ethanol
-
isoenzyme I from leaf
28
ethanol
-
isoenzyme TT-ADH
31
ethanol
pH 11.0, temperature not specified in the publication
33
ethanol
isozyme ADH2, at 21-23°C
36
ethanol
-
isoenzyme beta3,beta3
37.6
ethanol
cosubstrate NADP+, pH 8.0, 60°C
39
ethanol
-
pH 7.5, 25°C, Cu-ADH
39.7
ethanol
pH 7.0, temperature not specified in the publication
41
ethanol
-
in presence of 15 mM SDS
49.5
ethanol
pH 7.0, temperature not specified in the publication
50
ethanol
-
in presence of 50 mM SDS
53
ethanol
-
pH 7.5, 25°C, native Zn-ADH
64
ethanol
Sporotrichum pulverulentum
-
-
66
ethanol
-
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
184
ethanol
-
carboxymethylated enzyme, at 55°C
255
ethanol
-
recombinant isozyme ADH4, pH 10.5, 25°C
340
ethanol
-
isoenzyme ADH-1, pH 10.0
1625
ethanol
-
recombinant isozyme ADH4, pH 7.5, 25°C
5000
ethanol
-
isoenzyme ADH-1, pH 7.5
1
ethyl benzoylformate

-
65°C
4.2
ethyl benzoylformate
-
65°C, pH 5.0
5.3
ethyl benzoylformate
65°C, pH 5.5
1
ethyl oxo(phenyl)acetate
-
pH 10.5, 65°C
-
50
ethylene glycol

-
isoenzyme alpha,gamma1
200
ethylene glycol
-
above
4.46
formaldehyde

-
-
2.58
glycerol

-
-
0.0007
Hexanol

-
-
0.006
Hexanol
-
recombinant isozyme ADH4, pH 7.5, 25°C
0.085
Hexanol
-
recombinant isozyme ADH1, pH 7.5, 25°C
0.63
Hexanol
-
recombinant isozyme ADH4, pH 10.5, 25°C
1.9
Hexanol
-
recombinant isozyme ADH1, pH 10.5, 25°C
6.5
Hexanol
-
mutant enzyme W54L
13.8
Hexanol
-
wilde-type enzyme ADh1
0.26
Isobutyraldehyde

-
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.33
Isobutyraldehyde
-
mutant enzyme W95L, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.76
Isobutyraldehyde
-
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
9
Isopropanol

-
-
20.3
Isopropanol
at pH 7.0 and 25°C
0.78
methanol

-
-
3.04
methanol
-
33°C, pH 8
12
methanol
-
pyrazole-sensitive enzyme, pH 7.5
14
methanol
-
pyrazole-sensitive enzyme, pH 10.0
150
methanol
-
isoenzyme alpha,gamma1
2.7
methyl oxo(phenyl)acetate

-
pH 10.5, 65°C
2.7
methylbenzoylformate
-
65°C
0.13
N-benzyl-3-pyrrolidinone

-
pH 6.0
0.58
N-benzyl-3-pyrrolidinone
-
-
0.03
n-butanol

-
isoenzyme BB-ADH
0.07
n-butanol
-
isoenzyme AA-ADH
0.611
n-butanol
-
wild-type, pH 8.0, 60°C
2.6
n-butanol
-
isoenzyme TT-ADH
35.1
n-butanol
-
33°C, pH 8
0.02
n-Hexanol

-
isoenzyme TT-ADH
0.04
n-Hexanol
-
isoenzyme BB-ADH
0.26
n-Hexanol
-
isoenzyme TT-ADH
0.19
n-Propanol

2 mM zinc sulfate in 100 mM glycine-NaOH (pH 10.5) at 65°C
7.49
n-Propanol
-
33°C, pH 8
0.001
NAD+

-
pH 8.0, 60°C
0.0074
NAD+
-
isoenzyme beta2,beta2
0.0074
NAD+
-
isoenzyme beta1,beta1
0.0079
NAD+
-
isoenzyme gamma1,gamma1
0.0087
NAD+
-
isoenzyme gamma2,gamma2
0.011
NAD+
-
oxidation of ethanol
0.013
NAD+
-
isoenzyme alpha,alpha
0.028
NAD+
-
oxidation of propan-2-ol
0.037
NAD+
-
wild-type enzyme ADH1-1S
0.038
NAD+
-
mutant enzyme ADH1-1S1108
0.04
NAD+
-
isoenzyme ADH-2, pH 10.0 and pH 7.5
0.04
NAD+
-
isozyme ADH II, pH 8.5, 25°C
0.05
NAD+
-
isoenzyme ADH-1, pH 7.5
0.05
NAD+
-
pH 9.0, 60°C, recombinant enzyme
0.063
NAD+
-
recombinant allozyme Ile308, pH 7.5, 25°C
0.073
NAD+
-
enzyme form ADH I
0.074
NAD+
-
oxidation of ethanol
0.074
NAD+
-
recombinant allozyme Val308, pH 7.5, 25°C
0.089
NAD+
cosubstrate 1-phenylethanol, pH 9.0, 70°C
0.1
NAD+
-
isoenzyme ADH-3
0.1
NAD+
at pH 9.0 in bis-Tris propane buffer
0.11
NAD+
-
enzyme form ADH II
0.127
NAD+
cosubstrate 2,3-butanediol, pH 9.0, 70°C
0.13
NAD+
in 200 mM bicine, pH 9.0, at 60°C
0.17
NAD+
Sporotrichum pulverulentum
-
-
0.18
NAD+
-
isoenzyme beta1,beta1
0.18
NAD+
-
65°C, pH 10.5
0.2
NAD+
-
isoenzyme ADH-1, pH 7.5
0.2
NAD+
-
native enzyme, at 55°C
0.2
NAD+
-
in 0.1 M glycine-NaOH, pH 9.2, at 65°C
0.2
NAD+
-
mutant W87A, pH 7.0, 30°C
0.23
NAD+
-
oxidation of ethanol
0.239
NAD+
-
wild-type, pH 8.0, 60°C
0.24
NAD+
-
65°C; pH 10.5, 65°C
0.25
NAD+
-
isoenzyme ADH-1, pH 10.0
0.29
NAD+
pH 11.0, temperature not specified in the publication
0.39
NAD+
at pH 7.0 and 25°C
0.44
NAD+
65°C, pH 8.0; pH 8.0, 65°C
0.5
NAD+
-
carboxymethylated enzyme, at 55°C
0.5
NAD+
-
wild type enzyme, at 65°C, in the presence of benzyl alcohol
0.5
NAD+
-
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.5
NAD+
-
mutant Y25A/W49F/W167Y, 30°C, pH not specified in the publication
0.5
NAD+
mutant A25Y, pH 7.0, 30°C
0.9
NAD+
-
reduction of ethanol
0.9
NAD+
-
mutant Y25A/W49F/W87F, 30°C, pH not specified in the publication
1
NAD+
-
mutant Y25A, 30°C, pH not specified in the publication
1
NAD+
-
mutant Y25A, pH 7.0, 30°C
1.1
NAD+
-
wild-type, 30°C, pH not specified in the publication
1.1
NAD+
-
wild-type, pH 7.0, 30°C
1.2
NAD+
-
isozyme ADH I, pH 8.5, 25°C
1.3
NAD+
-
mutant W49F/W87F, 30°C, pH not specified in the publication
1.44
NAD+
cosubstrate 1-octanol, pH 8.0, 60°C
1.51
NAD+
cosubstrate ethanol, pH 8.0, 60°C
1.8
NAD+
-
mutant W49F/W167Y, 30°C, pH not specified in the publication
4
NAD+
-
mutant enzyme W95L, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
7.4
NAD+
-
isoenzyme beta1,beta1
8.3
NAD+
-
mutant W49F/W167Y/V260A, 30°C, pH not specified in the publication
9.7
NAD+
-
mutant Y25A/W49F/W167Y/V260A, 30°C, pH not specified in the publication
10
NAD+
-
mutant V260A, 30°C, pH not specified in the publication
10.2
NAD+
-
mutant W49F/W87F/V260A, 30°C, pH not specified in the publication
12.4
NAD+
-
mutant enzyme N249Y, at 65°C, in the presence of benzyl alcohol
14.8
NAD+
-
mutant Y25A/W49F/W87F/V260A, 30°C, pH not specified in the publication
30
NAD+
-
isoenzyme II from leaf
31
NAD+
-
isoenzyme III from leaf
40
NAD+
-
enzyme from liver
50
NAD+
-
Km above 50 mM, mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
60
NAD+
-
enzyme from caecum
73
NAD+
-
isoenzyme I from leaf
180
NAD+
-
isoenzyme beta2,beta2
550
NAD+
-
reduction of propan-2-ol
710
NAD+
-
isoenzyme beta3,beta3
0.0004
NADH

-
pH 8.0, 60°C
0.0017
NADH
-
isoenzyme ADH-2, pH 7.5
0.0035
NADH
-
pH 10.5, 65°C
0.0051
NADH
-
reduction of octanal
0.0064
NADH
-
isoenzyme beta1,beta1
0.007
NADH
-
isoenzyme gamma1,gamma1
0.008
NADH
-
mutant enzyme W95L, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.01
NADH
in 200 mM bicine, pH 6.0, at 60°C
0.01
NADH
-
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.011
NADH
-
isoenzyme alpha,alpha
0.012
NADH
-
enzyme form ADH II
0.013
NADH
-
wild type enzyme, at 65°C, in the presence of benzaldehyde
0.016
NADH
-
pH 7.7, 60°C
0.02
NADH
-
reduction of acetaldehyde
0.021
NADH
Sporotrichum pulverulentum
-
-
0.027
NADH
-
enzyme form ADH I
0.03
NADH
-
isozyme ADH II, pH 6.0, 25°C
0.03
NADH
-
native enzyme, at 55°C
0.03
NADH
-
in 50 mM Tris-HCl (pH 7.5) containing 0.25 mM benzaldehyde, at 65°C
0.038
NADH
wild-type, 60°C, pH 6.0, presence of 0.1 mM Zn2+
0.04
NADH
-
carboxymethylated enzyme, at 55°C
0.042
NADH
-
mutant enzyme ADH1-1S1108
0.048
NADH
-
25°C, pH 7.0
0.062
NADH
alcohol dehydrogenase domain, 60°C, pH 6.0, presence of 0.1 mM Zn2+
0.071
NADH
pH 6.0, temperature not specified in the publication
0.072
NADH
-
wild-type, pH 8.0, 60°C
0.085
NADH
-
wild-type enzyme ADH1-1S
0.097
NADH
-
pH 6.1, 70°C
0.105
NADH
-
isoenzyme beta2,beta2
0.105
NADH
cosubstrate acetoin, pH 6.0, 70°C
0.113
NADH
cosubstrate acetoin, pH 6.0, 70°C
0.16
NADH
65°C, pH 5.5; pH 5.5, 65°C
0.2
NADH
-
isozyme ADH I, pH 5.5, 25°C
0.2
NADH
-
pH 8.0, 60°C, recombinant enzyme
0.21
NADH
-
mutant enzyme N249Y, at 65°C, in the presence of benzaldehyde
0.75
NADH
-
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
1.4
NADH
cosubstrate acetaldehyde, pH 8.0, 60°C
6.4
NADH
-
isoenzyme beta1,beta1
25
NADH
-
isoenzyme III from leaf
70
NADH
-
isoenzyme II from leaf
105
NADH
-
isoenzyme beta2,beta2
121
NADH
-
isoenzyme I from leaf
260
NADH
-
isoenzyme beta3,beta3
0.066
NADP+

cosubstrate 1-phenylethanol, pH 9.0, 70°C
0.113
NADP+
cosubstrate 2,3-butanediol, pH 9.0, 70°C
0.28
NADP+
cosubstrate ethanol, pH 8.0, 60°C
2.4
NADP+
cosubstrate 1-octanol, pH 8.0, 60°C
0.6
NADPH

pH 6.5, 65°C
0.84
NADPH
cosubstrate octanal, pH 8.0, 60°C
0.043
octanal

-
enzyme form ADH-2
0.3
octanal
-
isoenzyme ADH-1, pH 7.5
1.15
octanal
cosubstrate NADPH, pH 8.0, 60°C
3.5
octanal
-
isoenzyme ADH-1, pH 7.5
9.6
octanal
isozyme ADH3, at 21-23°C
0.0014
Octanol

-
-
0.009
Octanol
-
recombinant allozyme Ile308, pH 7.5, 25°C
0.016
Octanol
-
recombinant allozyme Val308, pH 7.5, 25°C
0.08
Octanol
-
isoenzyme alpha,gamma1
0.6
Octanol
pH and temperature not specified in the publication
1.6
Octanol
-
isoenzyme ADH-2, pH 7.5
2.9
p-Methoxybenzaldehyde

-
isozyme ADH1, in the presence of 0.1 mM NADH, in 83 mM potassium phosphate, 40 mM KCl, and 0.25 mM EDTA buffer, pH 7.3, at 30°C
3.4
p-Methoxybenzaldehyde
in the presence of 0.1 mM NADH, in 83 mM potassium phosphate, 40 mM KCl, and 0.25 mM EDTA buffer, pH 7.3, at 30°C
5.9
p-Methoxybenzaldehyde
-
isozyme ADH2, in the presence of 0.1 mM NADH, in 83 mM potassium phosphate, 40 mM KCl, and 0.25 mM EDTA buffer, pH 7.3, at 30°C
6.9
p-methoxybenzyl alcohol

-
isozyme ADH2, in the presence of 2 mM NAD+, in 83 mM potassium phosphate, 40 mM KCl, and 0.25 mM EDTA buffer, pH 7.3, at 30°C
11
p-methoxybenzyl alcohol
-
isozyme ADH1, in the presence of 2 mM NAD+, in 83 mM potassium phosphate, 40 mM KCl, and 0.25 mM EDTA buffer, pH 7.3, at 30°C
22
p-methoxybenzyl alcohol
in the presence of 2 mM NAD+, in 83 mM potassium phosphate, 40 mM KCl, and 0.25 mM EDTA buffer, pH 7.3, at 30°C
0.2
pentan-1-ol

-
enzyme form ADH-3
0.012
Pentanol

-
pyrazole-sensitive enzyme, pH 7.5
0.018
Pentanol
-
pyrazole-sensitive enzyme, pH 10.0
11.8
Pentanol
-
mutant enzyme W54L
56.2
Pentanol
-
wilde-type enzyme ADh1
0.19
phenylacetaldehyde

-
pH 6.0, 37°C, recombinant enzyme expressed from Saccharomyces cerevisiae
0.26
phenylacetaldehyde
-
-
0.33
phenylacetaldehyde
-
pH 6.0, 37°C, recombinant enzyme expressed from Hansenula polymorpha
0.91
phenylacetaldehyde
-
pH 6.0, 37°C, recombinant enzyme expressed from Arxula adeninivorans
24.45
phenylglyoxylic acid

-
soluble enzyme
37.55
phenylglyoxylic acid
-
enzyme covalently immobilized to magnetic Fe3O4 nanoparticles via glutaraldehyde
0.019
propan-1-ol

-
-
0.5
propan-2-ol

-
-
64
propan-2-ol
Sporotrichum pulverulentum
-
-
193
propan-2-ol
-
mutant enzyme W54L
0.91
Propanol

-
-
13.6
Propanol
-
wilde-type enzyme ADh1
43.9
Propanol
-
mutant enzyme W54L
0.011
retinol

-
recombinant allozyme Ile308, pH 7.5, 25°C
0.012
retinol
-
recombinant allozyme Val308, pH 7.5, 25°C
0.009
trans-cinnamaldehyde

-
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.09
trans-cinnamaldehyde
-
mutant enzyme W95L,, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.23
trans-cinnamaldehyde
-
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.17
Valeraldehyde

-
pH 6.0, 37°C, recombinant enzyme expressed from Saccharomyces cerevisiae
0.28
Valeraldehyde
-
pH 6.0, 37°C, recombinant enzyme expressed from Arxula adeninivorans
2
Valeraldehyde
-
pH 6.0, 37°C, recombinant enzyme expressed from Hansenula polymorpha
0.034
Vanillyl alcohol

-
-
additional information
additional information

-
Km values for the class I isoenzymes with the substrates ethanol, methanol, ethylene glycol, benzyl alcohol, octanol, cyclohexanol and 16-hydroxyhexadecanoic acid
-
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
Km-values of active-site Co(II)substituted enzyme
-
additional information
additional information
-
kinetics of native and modified enzyme with coenzyme analogues
-
additional information
additional information
-
kinetics of ethanol oxidation
-
additional information
additional information
-
kinetics of ethanol oxidation
-
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
pH-dependence of Km-value
-
additional information
additional information
-
Michaelis-Menten kinetics
-
additional information
additional information
-
detailed kinetic mechanism, steady-state kinetics for wild-type and mutant enzymes, investigation of pH-dependency
-
additional information
additional information
-
steady-state kinetics, MW 25 kDa
-
additional information
additional information
-
steady-state kinetics
-
additional information
additional information
-
kinetics
-
additional information
additional information
-
Km for isozymes ADH1B1, ADH1B2, and ADH4 for all retinoid substrates in forward and reverse reaction
-
additional information
additional information
-
Km for isozymes ADH1, and ADH4 for all retinoid substrates in forward and reverse reaction
-
additional information
additional information
-
wild-type and mutant forms of the 3 isozymes, steady-state kinetics, detailed kinetic analysis, at different pH values and temperatures
-
additional information
additional information
-
kinetics
-
additional information
additional information
-
steady-state kinetics, kinetic mechanism
-
additional information
additional information
-
detailed kinetics, computational analysis of the reaction mechanism
-
additional information
additional information
-
effects of tert-butanol, butyramide, valeramide and capronamide on KM-value for ethanol
-
additional information
additional information
-
Michaelis-Menten kinetics
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
101
(2E)-but-2-en-1-ol
Yokenella sp.
W6CX26
pH 8.0, 55°C
-
405
(2E)-but-2-enal
Yokenella sp.
W6CX26
pH 6.5, 65°C
7.1
(R)-1-indanol
Sulfolobus acidocaldarius
Q4J702
65°C, pH 8.0; pH 8.0, 65°C
0.053
(R)-2-octanol
Equus caballus
-
-
1.01 - 1.9
(R)-2-pentanol
13.5
(R)-alpha-tetralol
Sulfolobus acidocaldarius
Q4J702
65°C, pH 8.0; pH 8.0, 65°C
61.4
(S)-(+)-1-indanol
Thermus thermophilus
-
65°C
57
(S)-(+)-alpha-tetraol
Thermus thermophilus
-
65°C
1.1
(S)-(-)-1-phenylethanol
Thermus thermophilus
-
65°C
13.7 - 61.4
(S)-1-indanol
1.1
(S)-1-phenylethanol
Thermus thermophilus
-
pH 10.5, 65°C
1.52
(S)-2-octanol
Equus caballus
-
-
0.87 - 6.7
(S)-2-pentanol
2.1 - 57
(S)-alpha-tetralol
8.3
1-Indanone
Thermus thermophilus
-
65°C; pH 10.5, 65°C
17.1
1-phenyl-1,2-propandione
Thermus thermophilus
-
pH 10.5, 65°C
3.1 - 17.1
1-phenyl-1,2-propanedione
35.7
1-phenylethanol
Thermococcus kodakarensis
Q5JI09
pH 9.0, 70°C
0.021
11-cis-retinal
Equus caballus
-
reduction with NADH
0.022
11-cis-retinol
Equus caballus
-
oxidation with NAD+
1.03 - 20
12-hydroxydodecanoate
0.333
12-Hydroxydodecanoic acid
Homo sapiens
-
-
27.6 - 34.3
12-hydroxylauric acid methyl ester
-
308
12-oxolauric acid methyl ester
Geobacillus stearothermophilus
-
wild-type, pH 8.0, 60°C
-
6.17
16-hydroxyhexadecanoate
Homo sapiens
-
-
3.2
2',3',4',5',6'-pentafluoroacetophenone
Sulfolobus acidocaldarius
-
65°C, pH 5.0
1.7 - 25.5
2,2,2-trifluoroacetophenone
0.65
2,2-dichloroacetophenone
Sulfolobus acidocaldarius
-
65°C, pH 5.0
43.8
2,3-Butanediol
Thermococcus kodakarensis
Q5JI09
pH 9.0, 70°C
0.12
2-butanone
Equus caballus
-
-
0.4
2-decanone
Aeropyrum pernix
-
pH 8.0, 60°C
2.83
2-deoxy-D-ribose
Homo sapiens
-
-
1.6
2-ethoxyethanol
Sulfolobus solfataricus
-
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.73
2-Heptanone
Aeropyrum pernix
-
pH 8.0, 60°C
1.08
2-Hexanone
Aeropyrum pernix
-
pH 8.0, 60°C
0.7 - 1
2-nonanone
Aeropyrum pernix
-
pH 8.0, 60°C
0.74
2-Octanone
Aeropyrum pernix
-
pH 8.0, 60°C
0.6
2-Pentanol
Aeropyrum pernix
-
pH 8.0, 60°C
0.0713 - 0.77
2-Pentanone
0.22 - 1.22
3,4-dihydro-retinal
0.088 - 2.5
3,4-dihydro-retinol
1.2 - 2
3-bromobenzyl alcohol
3.1
3-Methoxybenzaldehyde
Thermus thermophilus
-
65°C
1.4 - 16
3-Methoxybenzyl alcohol
1.2 - 1.4
3-methoxybenzylalcohol
0.08 - 0.27
3-methyl-1-butanol
7.72
3-methyl-cyclohexanol
Sulfolobus solfataricus
-
-
1.22
3-methylbutan-2-one
Sulfolobus solfataricus
-
-
2.17 - 3.8
3-methylcyclohexanone
2.05
3-oxo-5beta-androstan-17beta-ol
Equus caballus
-
-
0.36
3-Pentanone
Equus caballus
-
-
7.5
3-Phenyl-1-propanol
Homo sapiens
-
-
0.683
3-Pyridylcarbinol
Homo sapiens
-
pH 7.5, anodic enzyme form
1.1 - 3.5
4-bromobenzyl alcohol
0.7
4-carboxybenzaldehyde
145 - 432
4-chloroacetophenone
0.092 - 34.2
4-hydroxy-retinol
0.6 - 20.5
4-methoxybenzaldehyde
0.37 - 25.5
4-methoxybenzyl alcohol
1.5
4-methoxybenzylalcohol
Sulfolobus solfataricus
-
in the presence of 3 mM NAD+, in 0.1 M glycine-NaOH, pH 9.2, at 65°C
0.071
4-methoxyphenylacetone
Aeropyrum pernix
-
pH 8.0, 60°C
-
6.7 - 18.6
4-Methyl-1-pentanol
0.9
5beta-Pregnan-21-ol-3,20-dione hemisuccinate
Equus caballus
-
-
0.023
9-cis-retinal
Equus caballus
-
reduction with NADH
0.025
9-cis-retinol
Equus caballus
-
oxidation with NAD+
13.7
acetoin
Thermococcus kodakarensis
Q5JI09
pH 6.0, 70°C
0.35 - 1.9
all-trans retinol
0.018 - 2.5
all-trans-retinal
0.028 - 1.55
all-trans-retinol
48.1
alpha-tetralol
Thermus thermophilus
-
65°C
7.7
alpha-tetralone
Thermus thermophilus
-
65°C; pH 10.5, 65°C
0.983
Anisaldehyde
Sulfolobus solfataricus
-
-
1.6
benzil
Sulfolobus acidocaldarius
-
65°C, pH 5.0
0.35 - 93.3
benzyl alcohol
1.4
benzylalcohol
Sulfolobus solfataricus
-
in the presence of 3 mM NAD+, in 0.1 M glycine-NaOH, pH 9.2, at 65°C
1.93
butan-2-one
Sulfolobus solfataricus
-
-
0.94
Butyl alcohol
Equus caballus
-
-
55
cinnamaldehyde
Geobacillus stearothermophilus
-
pH 7.7, 60°C
43 - 133
cinnamyl alcohol
0.98
cis-decahydro-1-naphthol
Sulfolobus acidocaldarius
Q4J702
65°C, pH 8.0
0.167 - 15.9
Cyclohexanol
1.02
Cyclopentanone
Sulfolobus solfataricus
-
-
26
ethyl 3-methyl-2-oxobutyrate
Sulfolobus acidocaldarius
-
65°C, pH 5.0
1.9 - 50.1
ethyl benzoylformate
50.1
ethyl oxo(phenyl)acetate
Thermus thermophilus
-
pH 10.5, 65°C
-
3.97
ethyl pyruvate
Carboxydothermus hydrogenoformans
-
pH 6.5, 50°C, recombinant enzyme
0.75 - 4.5
ethylene glycol
56.6
hexaldehyde
Equus caballus
-
-
0.99
hexyl alcohol
Equus caballus
-
-
22
isatin
Sulfolobus acidocaldarius
Q4J702
65°C, pH 5.5; pH 5.5, 65°C
16.6
isoborneol
Sulfolobus acidocaldarius
-
65°C, pH 10.5
0.82 - 29
Isobutyraldehyde
0.58
Isopropanol
Equus caballus
-
-
6.2
methyl benzoylformate
Sulfolobus acidocaldarius
Q4J702
65°C, pH 5.5
9.2
methyl o-chlorobenzoylformate
Sulfolobus acidocaldarius
Q4J702
65°C, pH 5.5; pH 5.5, 65°C
38.1
methyl oxo(phenyl)acetate
2.63 - 8.58
methylcrotonyl alcohol
1.85
octan-1-ol
Coturnix coturnix
-
enzyme form ADH-3
0.24
p-nitrophenyl octanoate
Equus caballus
-
-
3.82
pentan2-ol
Sulfolobus solfataricus
-
-
202 - 303
phenylacetaldehyde
4.03
propan-1-ol
Sulfolobus solfataricus
-
-
41.9
propionaldehyde
Equus caballus
-
-
1.17
Propyl alcohol
Equus caballus
-
-
0.072
tert-butyl acetoacetate
Aeropyrum pernix
-
pH 8.0, 60°C
-
9.6
tetralin-1-ol
Sulfolobus acidocaldarius
-
65°C, pH 10.5
0.21 - 18.9
trans-cinnamaldehyde
1.83
tryptophol
Homo sapiens
-
-
0.467 - 8.67
Vanillyl alcohol
additional information
additional information
-
1
(R)-2-butanol

Sulfolobus solfataricus
-
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
1.7
(R)-2-butanol
Sulfolobus solfataricus
-
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
2
(R)-2-butanol
Equus caballus
-
-
1.01
(R)-2-pentanol

Equus caballus
-
-
1.1
(R)-2-pentanol
Sulfolobus solfataricus
-
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
1.9
(R)-2-pentanol
Sulfolobus solfataricus
-
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
13.7
(S)-1-indanol

Sulfolobus acidocaldarius
Q4J702
65°C, pH 8.0; pH 8.0, 65°C
61.4
(S)-1-indanol
Thermus thermophilus
-
pH 10.5, 65°C
1
(S)-2-butanol

Equus caballus
-
-
1.2
(S)-2-butanol
Sulfolobus solfataricus
-
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
2.17
(S)-2-butanol
Homo sapiens
-
-
4.8
(S)-2-butanol
Sulfolobus solfataricus
-
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.87
(S)-2-pentanol

Equus caballus
-
-
2.6
(S)-2-pentanol
Sulfolobus solfataricus
-
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
6.7
(S)-2-pentanol
Sulfolobus solfataricus
-
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
2.1
(S)-alpha-tetralol

Sulfolobus acidocaldarius
Q4J702
65°C, pH 8.0; pH 8.0, 65°C
57
(S)-alpha-tetralol
Thermus thermophilus
-
pH 10.5, 65°C
0.41
1-butanol

Aeropyrum pernix
-
pH 8.0, 60°C
0.833
1-butanol
Rattus norvegicus
-
isoenzyme ADH-3, pH 10.0
3.1
1-butanol
Sulfolobus solfataricus
-
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
7.5
1-butanol
Mesocricetus auratus
-
-
15.1
1-butanol
Sulfolobus solfataricus
-
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
48.8
1-butanol
Rattus norvegicus
-
isoenzyme ADH-1, pH 10.0
0.51
1-Heptanol

Sulfolobus solfataricus
-
mutant enzyme W95L, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
1.6
1-Heptanol
Sulfolobus solfataricus
-
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
11.9
1-Heptanol
Sulfolobus solfataricus
-
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.37
1-Hexanol

Aeropyrum pernix
-
pH 8.0, 60°C
0.42
1-Hexanol
Sulfolobus solfataricus
-
mutant enzyme W95L, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
2
1-Hexanol
Sulfolobus solfataricus
-
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
3.03
1-Hexanol
Mesocricetus auratus
-
-
13.3
1-Hexanol
Sulfolobus solfataricus
-
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
6.2
1-Indanol

Sulfolobus acidocaldarius
-
65°C, pH 10.5
45.7
1-Indanol
Thermus thermophilus
-
65°C
1
1-Octanol

Rattus norvegicus
-
isoenzyme ADH-3, pH 10.0
2.33
1-Octanol
Rattus norvegicus
-
isoenzyme ADH-2, pH 10.0
2.83
1-Octanol
Homo sapiens
-
-
60.8
1-Octanol
Rattus norvegicus
-
isoenzyme ADH-1, pH 10.0
260.7
1-Octanol
Geobacillus thermodenitrificans
A4IP64, A4ISB9
cosubstrate NADP+, pH 8.0, 60°C
0.183
1-Pentanol

Homo sapiens
-
-
0.45
1-Pentanol
Aeropyrum pernix
-
pH 8.0, 60°C
0.53
1-Pentanol
Sulfolobus solfataricus
-
mutant enzyme W95L, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
1.17
1-Pentanol
Rattus norvegicus
-
isoenzyme ADH-3, pH 10.0
2.5
1-Pentanol
Sulfolobus solfataricus
-
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
3.53
1-Pentanol
Rattus norvegicus
-
isoenzyme ADH-2, pH 10.0
16.3
1-Pentanol
Sulfolobus solfataricus
-
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
48.8
1-Pentanol
Rattus norvegicus
-
isoenzyme ADH-1, pH 10.0
3.1
1-phenyl-1,2-propanedione

Sulfolobus acidocaldarius
Q4J702
65°C, pH 5.5
5.3
1-phenyl-1,2-propanedione
Sulfolobus acidocaldarius
-
65°C, pH 5.0
17.1
1-phenyl-1,2-propanedione
Thermus thermophilus
-
65°C
0.26
1-propanol

Aeropyrum pernix
-
pH 8.0, 60°C
1.2
1-propanol
Sulfolobus solfataricus
-
native enzyme, at 55°C
1.2
1-propanol
Sulfolobus solfataricus
-
in the presence of 3 mM NAD+, in 0.1 M glycine-NaOH, pH 9.2, at 65°C
2.2
1-propanol
Sulfolobus solfataricus
-
carboxymethylated enzyme, at 55°C
2.5
1-propanol
Sulfolobus solfataricus
-
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
2.6
1-propanol
Sulfolobus solfataricus
-
wild type enzyme, at 65°C
15.7
1-propanol
Sulfolobus solfataricus
-
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
16.6
1-propanol
Sulfolobus solfataricus
-
mutant enzyme N249Y, at 65°C
1.03
12-hydroxydodecanoate

Mesocricetus auratus
-
isoenzyme TT-ADH
1.27
12-hydroxydodecanoate
Rattus norvegicus
-
isoenzyme ADH-3, pH 10.0
1.45
12-hydroxydodecanoate
Coturnix coturnix
-
enzyme form ADH-3
2.2
12-hydroxydodecanoate
Mesocricetus auratus
-
isoenzyme BB-ADH
2.43
12-hydroxydodecanoate
Mesocricetus auratus
-
-
2.77
12-hydroxydodecanoate
Mesocricetus auratus
-
isoenzyme AA-ADH
2.95
12-hydroxydodecanoate
Homo sapiens
-
-
3.03
12-hydroxydodecanoate
Homo sapiens
-
-
3.83
12-hydroxydodecanoate
Rattus norvegicus
-
isoenzyme ADH-12, pH 10.0
4
12-hydroxydodecanoate
Homo sapiens
-
-
20
12-hydroxydodecanoate
Rattus norvegicus
-
isoenzyme ADH-1, pH 10.0
27.6
12-hydroxylauric acid methyl ester

Geobacillus stearothermophilus
-
wild-type, pH 8.0, 60°C
-
34.3
12-hydroxylauric acid methyl ester
Geobacillus stearothermophilus
-
mutant C257L, pH 8.0, 60°C
-
0.018
13-cis-retinal

Equus caballus
-
reduction with NADH
0.018
13-cis-retinol
Equus caballus
-
oxidation with NAD+
1.7
2,2,2-trifluoroacetophenone

Sulfolobus acidocaldarius
-
65°C, pH 5.0
10
2,2,2-trifluoroacetophenone
Sulfolobus acidocaldarius
Q4J702
65°C, pH 5.5
25.5
2,2,2-trifluoroacetophenone
Thermus thermophilus
-
65°C; pH 10.5, 65°C
0.48
2-butanol

Aeropyrum pernix
-
pH 8.0, 60°C
4.75
2-butanol
Mesocricetus auratus
-
-
1.67
2-Buten-1-ol

Rattus norvegicus
-
isoenzyme ADH-3, pH 10.0
5.83
2-Buten-1-ol
Rattus norvegicus
-
isoenzyme ADH-2, pH 10.0
213
2-Buten-1-ol
Rattus norvegicus
-
isoenzyme ADH-1, pH 10.0
0.0713
2-Pentanone

Equus caballus
-
-
0.77
2-Pentanone
Aeropyrum pernix
-
pH 8.0, 60°C
0.24
2-propanol

Aeropyrum pernix
-
pH 8.0, 60°C
0.25
2-propanol
Sulfolobus solfataricus
-
native enzyme, at 55°C
0.25
2-propanol
Sulfolobus solfataricus
-
in the presence of 3 mM NAD+, in 0.1 M glycine-NaOH, pH 9.2, at 65°C
0.45
2-propanol
Sulfolobus solfataricus
-
carboxymethylated enzyme, at 55°C
0.75
2-propanol
Homo sapiens
-
-
287
2-propanol
Geobacillus stearothermophilus
-
pH 7.7, 60°C
0.22
3,4-dihydro-retinal

Homo sapiens
-
pH 7.5, 25°C, isozyme ADH1B2
1.22
3,4-dihydro-retinal
Homo sapiens
-
pH 7.5, 25°C, isozyme ADH4
0.088
3,4-dihydro-retinol

Homo sapiens
-
pH 7.5, 25°C, isozyme ADH1B2
2.5
3,4-dihydro-retinol
Homo sapiens
-
pH 7.5, 25°C, isozyme ADH4
1.2
3-bromobenzyl alcohol

Sulfolobus solfataricus
-
native enzyme, at 55°C
2
3-bromobenzyl alcohol
Sulfolobus solfataricus
-
carboxymethylated enzyme, at 55°C
1.2
3-bromobenzylalcohol
Sulfolobus solfataricus
-
in the presence of 3 mM NAD+, in 0.1 M glycine-NaOH, pH 9.2, at 65°C
1.4
3-Methoxybenzyl alcohol

Sulfolobus solfataricus
-
native enzyme, at 55°C
16
3-Methoxybenzyl alcohol
Sulfolobus solfataricus
-
carboxymethylated enzyme, at 55°C
1.2
3-methoxybenzylalcohol

Sulfolobus solfataricus
-
in the presence of 5 mM benzylalcohol, in 0.1 M glycine-NaOH, pH 9.2, at 65°C
1.4
3-methoxybenzylalcohol
Sulfolobus solfataricus
-
in the presence of 3 mM NAD+, in 0.1 M glycine-NaOH, pH 9.2, at 65°C
0.08
3-methyl-1-butanol

Equus caballus
-
mutant enzyme W54L
0.27
3-methyl-1-butanol
Equus caballus
-
wild-type enzyme Adh 1
2.17
3-methylcyclohexanone

Sulfolobus solfataricus
-
-
3.8
3-methylcyclohexanone
Sulfolobus acidocaldarius
Q4J702
65°C, pH 5.5
1.5
3-Pentanol

Sulfolobus solfataricus
-
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
2.26
3-Pentanol
Equus caballus
-
-
1.1
4-bromobenzyl alcohol

Sulfolobus solfataricus
-
native enzyme, at 55°C
3.5
4-bromobenzyl alcohol
Sulfolobus solfataricus
-
carboxymethylated enzyme, at 55°C
1.1
4-bromobenzylalcohol
Sulfolobus solfataricus
-
in the presence of 3 mM NAD+, in 0.1 M glycine-NaOH, pH 9.2, at 65°C
0.7
4-carboxybenzaldehyde

Sulfolobus solfataricus
-
native enzyme, at 55°C
0.7
4-carboxybenzaldehyde
Sulfolobus solfataricus
-
in 50 mM Tris-HCl (pH 7.5) containing 0.2 mM NADH + H+, at 65°C
145
4-chloroacetophenone

Rhodococcus ruber
-
pH 6.0, 37°C, recombinant enzyme expressed from Hansenula polymorpha
230
4-chloroacetophenone
Rhodococcus ruber
-
pH 6.0, 37°C, recombinant enzyme expressed from Saccharomyces cerevisiae
432
4-chloroacetophenone
Rhodococcus ruber
-
pH 6.0, 37°C, recombinant enzyme expressed from Arxula adeninivorans
0.092
4-hydroxy-retinol

Homo sapiens
-
pH 7.5, 25°C, isozyme ADH1B1
2.83
4-hydroxy-retinol
Homo sapiens
-
pH 7.5, 25°C, isozyme ADH1B2
34.2
4-hydroxy-retinol
Homo sapiens
-
pH 7.5, 25°C, isozyme ADH4
0.6
4-methoxybenzaldehyde

Sulfolobus solfataricus
-
native enzyme, at 55°C
0.6
4-methoxybenzaldehyde
Sulfolobus solfataricus
-
in 50 mM Tris-HCl (pH 7.5) containing 0.2 mM NADH + H+, at 65°C
3.1
4-methoxybenzaldehyde
Thermus thermophilus
-
pH 6.0, 65°C
3.3
4-methoxybenzaldehyde
Sulfolobus solfataricus
-
wild type enzyme, at 65°C
20.5
4-methoxybenzaldehyde
Sulfolobus solfataricus
-
mutant enzyme N249Y, at 65°C
0.37
4-methoxybenzyl alcohol

Sulfolobus solfataricus
-
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.6
4-methoxybenzyl alcohol
Aeropyrum pernix
-
pH 8.0, 60°C
1.5
4-methoxybenzyl alcohol
Sulfolobus solfataricus
-
native enzyme, at 55°C
1.6
4-methoxybenzyl alcohol
Thermus thermophilus
-
65°C; pH 10.5, 65°C
2.9
4-methoxybenzyl alcohol
Sulfolobus solfataricus
-
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
3.3
4-methoxybenzyl alcohol
Sulfolobus solfataricus
-
wild type enzyme, at 65°C
16.8
4-methoxybenzyl alcohol
Sulfolobus solfataricus
-
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
20.5
4-methoxybenzyl alcohol
Sulfolobus solfataricus
-
carboxymethylated enzyme, at 55°C
25.5
4-methoxybenzyl alcohol
Sulfolobus solfataricus
-
mutant enzyme N249Y, at 65°C
6.7
4-Methyl-1-pentanol

Equus caballus
-
wild-type enzyme Adh 1
18.6
4-Methyl-1-pentanol
Equus caballus
-
mutant enzyme W54L
4.6
4-Nitrobenzaldehyde

Sulfolobus solfataricus
-
native enzyme, at 55°C
4.6
4-Nitrobenzaldehyde
Sulfolobus solfataricus
-
in 50 mM Tris-HCl (pH 7.5) containing 0.2 mM NADH + H+, at 65°C
0.167
4-oxo-retinal

Homo sapiens
-
pH 7.5, 25°C, isozyme ADH1B1
7.83
4-oxo-retinal
Homo sapiens
-
pH 7.5, 25°C, isozyme ADH1B2
20
4-oxo-retinal
Homo sapiens
-
pH 7.5, 25°C, isozyme ADH4
0.3
acetaldehyde

Natronomonas pharaonis
-
40°C, pH 9.0, 50 mM Tris-HCl, 4 M NaCl
3.33
acetaldehyde
Saimiri sciureus
-
pyrazole-insensitive enzyme, pH 7.0
5.4
acetaldehyde
Natronomonas pharaonis
-
70°C, pH 9.0, 50 mM Tris-HCl, 4 M NaCl
16.7
acetaldehyde
Saimiri sciureus
-
pyrazole-sensitive enzyme, pH 7.0
31.8
acetaldehyde
Equus caballus
-
-
53
acetaldehyde
Kluyveromyces marxianus
G3FFC9, V9SCJ1, V9SDP6, V9SFA1
pH 7.0, temperature not specified in the publication
83
acetaldehyde
Geobacillus thermoglucosidasius
A0A0J9X1L6
wild-type, 60°C, pH 6.0, presence of 0.1 mM Zn2+
138.1
acetaldehyde
Geobacillus thermodenitrificans
A4IP64, A4ISB9
cosubstrate NADPH, pH 8.0, 60°C
283
acetaldehyde
Kluyveromyces marxianus
G3FFC9, V9SCJ1, V9SDP6, V9SFA1
pH 7.0, temperature not specified in the publication
350
acetaldehyde
Geobacillus thermoglucosidasius
A0A0J9X1L6
alcohol dehydrogenase domain, 60°C, pH 6.0, presence of 0.1 mM Zn2+
404.8
acetaldehyde
Geobacillus thermodenitrificans
A4IP64, A4ISB9
cosubstrate NADH, pH 8.0, 60°C
681
acetaldehyde
Geobacillus stearothermophilus
-
wild-type, pH 8.0, 60°C
3500
acetaldehyde
Ogataea angusta
H9ZGN0
pH 6.0, 22°C, recombinant enzyme
0.317
acetone

Sulfolobus solfataricus
-
-
0.33
acetone
Equus caballus
-
-
211
acetophenone

Rhodococcus ruber
-
pH 6.0, 37°C, recombinant enzyme expressed from Hansenula polymorpha
259
acetophenone
Rhodococcus ruber
-
pH 6.0, 37°C, recombinant enzyme expressed from Saccharomyces cerevisiae
288
acetophenone
Rhodococcus ruber
-
pH 6.0, 37°C, recombinant enzyme expressed from Arxula adeninivorans
0.35
all-trans retinol

Mesocricetus auratus
-
isoenzyme AA-ADH
1.3
all-trans retinol
Mesocricetus auratus
-
-
1.3
all-trans retinol
Mesocricetus auratus
-
isoenzyme TT-ADH
1.9
all-trans retinol
Mesocricetus auratus
-
isoenzyme BB-ADH
0.018
all-trans-retinal

Homo sapiens
-
pH 7.5, 25°C, isozyme ADH1B1
0.022
all-trans-retinal
Equus caballus
-
oxidation with NAD+
0.55
all-trans-retinal
Homo sapiens
-
pH 7.5, 25°C, isozyme ADH1B2
1.83
all-trans-retinal
Homo sapiens
-
pH 7.5, 25°C, isozyme ADH4
2.5
all-trans-retinal
Equus caballus
-
reduction with NADH
0.028
all-trans-retinol

Homo sapiens
-
pH 7.5, 25°C, isozyme ADH1B1
0.25
all-trans-retinol
Homo sapiens
-
pH 7.5, 25°C, isozyme ADH1B2
0.4
all-trans-retinol
Homo sapiens
-
-
0.9
all-trans-retinol
Homo sapiens
-
pH 7.5, 25°C, isozyme ADH4
1.55
all-trans-retinol
Equus caballus
-
oxidation with NAD+
0.36
benzaldehyde

Sulfolobus solfataricus
-
mutant enzyme W95L, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
1.22
benzaldehyde
Aeropyrum pernix
-
pH 8.0, 60°C
1.8
benzaldehyde
Sulfolobus solfataricus
-
carboxymethylated enzyme, at 55°C
3.5
benzaldehyde
Sulfolobus solfataricus
-
native enzyme, at 55°C
3.5
benzaldehyde
Sulfolobus solfataricus
-
in 50 mM Tris-HCl (pH 7.5) containing 0.2 mM NADH + H+, at 65°C
11.3
benzaldehyde
Sulfolobus solfataricus
-
wild type enzyme, at 65°C
11.3
benzaldehyde
Sulfolobus solfataricus
-
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
13.1
benzaldehyde
Sulfolobus solfataricus
-
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
25.5
benzaldehyde
Sulfolobus solfataricus
-
mutant enzyme N249Y, at 65°C
29.5
benzaldehyde
Equus caballus
-
-
360
benzaldehyde
Yokenella sp.
W6CX26
pH 6.5, 65°C
0.35
benzyl alcohol

Sulfolobus solfataricus
-
mutant enzyme W95L, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.78
benzyl alcohol
Equus caballus
-
-
1
benzyl alcohol
Rattus norvegicus
-
isoenzyme ADH-3, pH 10.0
1.02
benzyl alcohol
Aeropyrum pernix
-
pH 8.0, 60°C
1.37
benzyl alcohol
Mesocricetus auratus
-
-
1.4
benzyl alcohol
Sulfolobus solfataricus
-
native enzyme, at 55°C
1.68
benzyl alcohol
Sulfolobus solfataricus
-
-
2.52
benzyl alcohol
Coturnix coturnix
-
enzyme form ADH-3
3.6
benzyl alcohol
Sulfolobus solfataricus
-
wild type enzyme, at 65°C
3.6
benzyl alcohol
Sulfolobus solfataricus
-
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
3.8
benzyl alcohol
Moraxella sp.
P81786
mutant A25Y, pH 7.0, 30°C
4.67
benzyl alcohol
Homo sapiens
-
isoenzyme alpha,gamma1
5.5
benzyl alcohol
Geobacillus stearothermophilus
-
mutant W87A, pH 7.0, 30°C
6.9
benzyl alcohol
Moraxella sp.
P81786
wild-type, pH 7.0, 30°C
9.17
benzyl alcohol
Homo sapiens
-
-
14
benzyl alcohol
Geobacillus stearothermophilus
-
mutant Y25A, pH 7.0, 30°C
14.5
benzyl alcohol
Sulfolobus solfataricus
-
carboxymethylated enzyme, at 55°C
20
benzyl alcohol
Sulfolobus solfataricus
-
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
21.7
benzyl alcohol
Sulfolobus solfataricus
-
mutant enzyme N249Y, at 65°C
24.9
benzyl alcohol
Geobacillus stearothermophilus
-
wild-type, pH 7.0, 30°C
89.7
benzyl alcohol
Rattus norvegicus
-
isoenzyme ADH-1, pH 10.0
93.3
benzyl alcohol
Yokenella sp.
W6CX26
pH 8.0, 55°C
1.97
butan-1-ol

Sulfolobus solfataricus
-
-
7.22
butan-1-ol
Coturnix coturnix
-
enzyme form ADH-3
0.31
butan-2-ol

Equus caballus
-
mutant enzyme W54L
3.55
butan-2-ol
Sulfolobus solfataricus
-
-
11.9
butan-2-ol
Equus caballus
-
wild-type enzyme Adh 1
0.0833
butanol

Saimiri sciureus
-
pyrazole-insensitive enzyme, pH 7.5
0.35
butanol
Saimiri sciureus
-
pyrazole-sensitive enzyme, pH 7.5
0.45
butanol
Saimiri sciureus
-
pyrazole-sensitive enzyme and pyrazole-insensitive enzyme, pH 10.0
0.7
butanol
Homo sapiens
-
pH 7.5, anodic enzyme form
1.1
butanol
Alligator mississippiensis
-
-
32.7
butanol
Equus caballus
-
mutant enzyme W54L
34.8
butanol
Homo sapiens
-
-
118
butanol
Equus caballus
-
wild-type enzyme Adh 1
0.36
Butyraldehyde

Sulfolobus solfataricus
-
mutant enzyme W95L, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
19.7
Butyraldehyde
Sulfolobus solfataricus
-
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C; wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
35.4
Butyraldehyde
Equus caballus
-
-
931
Butyraldehyde
Geobacillus stearothermophilus
-
wild-type, pH 8.0, 60°C
317
Capronaldehyde

Rhodococcus ruber
-
pH 6.0, 37°C, recombinant enzyme expressed from Arxula adeninivorans
410
Capronaldehyde
Rhodococcus ruber
-
pH 6.0, 37°C, recombinant enzyme expressed from Hansenula polymorpha
576
Capronaldehyde
Rhodococcus ruber
-
pH 6.0, 37°C, recombinant enzyme expressed from Saccharomyces cerevisiae
43
cinnamyl alcohol

Geobacillus stearothermophilus
-
pH 7.7, 60°C
98
cinnamyl alcohol
Equus caballus
-
mutant enzyme W54L
133
cinnamyl alcohol
Equus caballus
-
wild-type enzyme Adh 1
4.4
cycloheptanol

Sulfolobus acidocaldarius
Q4J702
65°C, pH 8.0; pH 8.0, 65°C
7
cycloheptanol
Sulfolobus acidocaldarius
-
65°C, pH 10.5
0.167
Cyclohexanol

Alligator mississippiensis
-
-
0.383
Cyclohexanol
Rattus norvegicus
-
isoenzyme ADH-2, pH 10.0
0.52
Cyclohexanol
Aeropyrum pernix
-
pH 8.0, 60°C
0.583
Cyclohexanol
Homo sapiens
-
-
0.65
Cyclohexanol
Sulfolobus solfataricus
-
native enzyme, at 55°C
0.65
Cyclohexanol
Sulfolobus solfataricus
-
in the presence of 3 mM NAD+, in 0.1 M glycine-NaOH, pH 9.2, at 65°C
0.75
Cyclohexanol
Coturnix coturnix
-
enzyme form ADH-2
1.2
Cyclohexanol
Sulfolobus solfataricus
-
carboxymethylated enzyme, at 55°C
1.2
Cyclohexanol
Sulfolobus solfataricus
-
wild type enzyme, at 65°C
1.2
Cyclohexanol
Sulfolobus solfataricus
-
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
1.5
Cyclohexanol
Rattus norvegicus
-
isoenzyme ADH-3, pH 10.0
1.75
Cyclohexanol
Rattus norvegicus
-
isoenzyme ADH-1, pH 10.0
1.75
Cyclohexanol
Mesocricetus auratus
-
isoenzyme BB-ADH
2.03
Cyclohexanol
Mesocricetus auratus
-
-
2.4
Cyclohexanol
Mesocricetus auratus
-
isoenzyme AA-ADH
3.55
Cyclohexanol
Coturnix coturnix
-
enzyme form ADH-3
8.5
Cyclohexanol
Mesocricetus auratus
-
isoenzyme TT-ADH
12.4
Cyclohexanol
Sulfolobus solfataricus
-
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
15.9
Cyclohexanol
Sulfolobus solfataricus
-
mutant enzyme N249Y, at 65°C
1.27
cyclohexanone

Aeropyrum pernix
-
pH 8.0, 60°C
5.9
cyclohexanone
Equus caballus
-
-
0.00086
ethanol

Picrophilus torridus
Q6L0S1
-
0.0833
ethanol
Saimiri sciureus
-
pyrazole-insensitive enzyme, pH 7.5
0.167
ethanol
Homo sapiens
-
recombinant allozyme Ile308, pH 7.5, 25°C
0.175
ethanol
Homo sapiens
-
recombinant allozyme Val308, pH 7.5, 25°C
0.23
ethanol
Aeropyrum pernix
-
pH 8.0, 60°C
0.4
ethanol
Saimiri sciureus
-
pyrazole-sensitive enzyme, pH 7.5
0.4
ethanol
Natronomonas pharaonis
-
40°C, pH 9.0, 50 mM Tris-HCl, 4 M NaCl
0.45
ethanol
Saimiri sciureus
-
pyrazole-insensitive enzyme, pH 10.0
0.467
ethanol
Alligator mississippiensis
-
-
0.5
ethanol
Sulfolobus solfataricus
-
native enzyme, at 55°C
0.5
ethanol
Sulfolobus solfataricus
-
in the presence of 3 mM NAD+, in 0.1 M glycine-NaOH, pH 9.2, at 65°C
0.567
ethanol
Saimiri sciureus
-
pyrazole-sensitive enzyme
0.7
ethanol
Homo sapiens
-
pH 7.5, anodic enzyme form
0.85
ethanol
Sulfolobus solfataricus
-
carboxymethylated enzyme, at 55°C
1
ethanol
Rattus norvegicus
-
isoenzyme ADH-3, pH 10.0
1.27
ethanol
Equus caballus
-
-
1.88
ethanol
Sulfolobus solfataricus
-
-
1.92
ethanol
Mus musculus
-
recombinant isozyme ADH1, pH 7.5, 25°C
2.5
ethanol
Homo sapiens
-
per active site
2.83
ethanol
Mesocricetus auratus
-
isoenzyme AA-ADH
3.1
ethanol
Sulfolobus solfataricus
-
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
3.38
ethanol
Mesocricetus auratus
-
isoenzyme BB-ADH
3.83
ethanol
Homo sapiens
-
isoenzyme gamma1,gamma1
4
ethanol
Homo sapiens
-
isoenzyme alpha,gamma1
4.1
ethanol
Sulfolobus solfataricus
-
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
4.42
ethanol
Mus musculus
-
recombinant isozyme ADH1, pH 10.5, 25°C
4.5
ethanol
Kluyveromyces marxianus
G3FFC9, V9SCJ1, V9SDP6, V9SFA1
pH 7.0, temperature not specified in the publication
4.92
ethanol
Coturnix coturnix
-
enzyme form ADH-2
6.63
ethanol
Coturnix coturnix
-
enzyme form ADH-3
7.83
ethanol
Homo sapiens
-
-
8
ethanol
Mesocricetus auratus
-
-
8.2
ethanol
Natronomonas pharaonis
-
70°C, pH 9.0, 50 mM Tris-HCl, 4 M NaCl
9.58
ethanol
Mesocricetus auratus
-
isoenzyme TT-ADH
10.2
ethanol
Homo sapiens
-
pH 10.0, anodic enzyme form
30.7
ethanol
Homo sapiens
-
-
33
ethanol
Kluyveromyces marxianus
G3FFC9, V9SCJ1, V9SDP6, V9SFA1
pH 7.0, temperature not specified in the publication
39.9
ethanol
Flavobacterium frigidimaris
Q8L3C9
10°C, pH 9.0
41.3
ethanol
Mus musculus
-
recombinant isozyme ADH4, pH 7.5, 25°C
62.48
ethanol
Flavobacterium frigidimaris
Q8L3C9
20°C, pH 9.0
62.7
ethanol
Rattus norvegicus
-
isoenzyme ADH-1, pH 10.0
102
ethanol
Equus caballus
-
mutant enzyme W54L
109.5
ethanol
Flavobacterium frigidimaris
Q8L3C9
30°C, pH 9.0
123
ethanol
Equus caballus
-
wild-type enzyme Adh 1
136.2
ethanol
Geobacillus thermodenitrificans
A4IP64, A4ISB9
cosubstrate NADP+, pH 8.0, 60°C
142
ethanol
Kluyveromyces marxianus
G3FFC9, V9SCJ1, V9SDP6, V9SFA1
pH 7.0, temperature not specified in the publication
143
ethanol
Saccharomyces cerevisiae
-
in 50 mM Tris-HCl, pH 8.0 at 25°C
158
ethanol
Kluyveromyces marxianus
G3FFC9, V9SCJ1, V9SDP6, V9SFA1
pH 7.0, temperature not specified in the publication
160.8
ethanol
Flavobacterium frigidimaris
Q8L3C9
40°C, pH 9.0
215
ethanol
Mus musculus
-
recombinant isozyme ADH4, pH 10.5, 25°C
251.3
ethanol
Saccharomyces cerevisiae
-
10°C, pH 9.0
262.3
ethanol
Flavobacterium frigidimaris
Q8L3C9
50°C, pH 9.0
305
ethanol
Geobacillus stearothermophilus
-
wild-type, pH 8.0, 60°C
308
ethanol
Equus caballus
-
wild-type enzyme Adh 1
365
ethanol
Geobacillus stearothermophilus
-
mutant C257L, pH 8.0, 60°C
432.3
ethanol
Saccharomyces cerevisiae
-
20°C, pH 9.0
474.9
ethanol
Flavobacterium frigidimaris
Q8L3C9
60°C, pH 9.0
638.7
ethanol
Geobacillus thermodenitrificans
A4IP64, A4ISB9
cosubstrate NAD+, pH 8.0, 60°C
786.1
ethanol
Saccharomyces cerevisiae
-
30°C, pH 9.0
895.4
ethanol
Saccharomyces cerevisiae
-
40°C, pH 9.0
3000
ethanol
Ogataea angusta
H9ZGN0
pH 9.0, 22°C, recombinant enzyme
1.9
ethyl benzoylformate

Sulfolobus acidocaldarius
-
65°C, pH 5.0
2.35
ethyl benzoylformate
Sulfolobus acidocaldarius
Q4J702
65°C, pH 5.5
50.1
ethyl benzoylformate
Thermus thermophilus
-
65°C
0.75
ethylene glycol

Homo sapiens
-
-
4.5
ethylene glycol
Pseudomonas aeruginosa
-
-
0.167
Hexanol

Alligator mississippiensis
-
-
0.45
Hexanol
Mus musculus
-
recombinant isozyme ADH1, pH 7.5, 25°C
3.83
Hexanol
Mus musculus
-
recombinant isozyme ADH1, pH 10.5, 25°C
19.5
Hexanol
Homo sapiens
-
-
23.9
Hexanol
Equus caballus
-
mutant enzyme W54L
29.6
Hexanol
Equus caballus
-
wild-type enzyme Adh 1
30.8
Hexanol
Mus musculus
-
recombinant isozyme ADH4, pH 7.5, 25°C
86.5
Hexanol
Mus musculus
-
recombinant isozyme ADH4, pH 10.5, 25°C
0.82
Isobutyraldehyde

Sulfolobus solfataricus
-
mutant enzyme W95L, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
18.1
Isobutyraldehyde
Sulfolobus solfataricus
-
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
29
Isobutyraldehyde
Sulfolobus solfataricus
-
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.05
methanol

Alligator mississippiensis
-
-
0.102
methanol
Homo sapiens
-
-
0.117
methanol
Saimiri sciureus
-
pyrazole-sensitive enzyme, pH 7.5
0.217
methanol
Saimiri sciureus
-
pyrazole-sensitive enzyme, pH 10.0
3.1
methanol
Sulfolobus solfataricus
-
-
38.1
methyl oxo(phenyl)acetate

Thermus thermophilus
-
pH 10.5, 65°C
38.1
methylbenzoylformate
Thermus thermophilus
-
65°C
2.63
methylcrotonyl alcohol

Mesocricetus auratus
-
isoenzyme BB-ADH
3
methylcrotonyl alcohol
Mesocricetus auratus
-
isoenzyme AA-ADH
8.58
methylcrotonyl alcohol
Mesocricetus auratus
-
isoenzyme TT-ADH
2.58
n-butanol

Mesocricetus auratus
-
isoenzymee BB-ADH
3.33
n-butanol
Mesocricetus auratus
-
isoenzyme AA-ADH
7.18
n-butanol
Mesocricetus auratus
-
isoenzyme TT-ADH
195
n-butanol
Geobacillus stearothermophilus
-
wild-type, pH 8.0, 60°C
2.35
n-Hexanol

Mesocricetus auratus
-
isoenzyme BB-ADH
2.8
n-Hexanol
Mesocricetus auratus
-
isoenzyme AA-ADH
4.08
n-Hexanol
Mesocricetus auratus
-
isoenzyme TT-ADH
0.4
NAD+

Aeropyrum pernix
-
pH 8.0, 60°C
0.52
NAD+
Sulfolobus solfataricus
-
mutant enzyme W95L, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.717
NAD+
Homo sapiens
-
pH 7.5, anodic enzyme form
0.84
NAD+
Thermus thermophilus
-
65°C; pH 10.5, 65°C
1
NAD+
Geobacillus stearothermophilus
-
mutant W49F/W87F/V260A, 30°C, pH not specified in the publication
1.4
NAD+
Sulfolobus solfataricus
-
native enzyme, at 55°C
1.4
NAD+
Sulfolobus solfataricus
-
in 0.1 M glycine-NaOH, pH 9.2, at 65°C
1.4
NAD+
Geobacillus stearothermophilus
-
mutant W49F/W167Y/V260A, 30°C, pH not specified in the publication
1.9
NAD+
Geobacillus stearothermophilus
-
mutant V260A, 30°C, pH not specified in the publication; mutant Y25A/W49F/W167Y/V260A, 30°C, pH not specified in the publication
2.5
NAD+
Geobacillus stearothermophilus
-
mutant Y25A/W49F/W167Y, 30°C, pH not specified in the publication; mutant Y25A/W49F/W87F/V260A, 30°C, pH not specified in the publication
3
NAD+
Geobacillus stearothermophilus
-
mutant W49F/W87F, 30°C, pH not specified in the publication
3.4
NAD+
Sulfolobus solfataricus
-
wild type enzyme, at 65°C, in the presence of benzyl alcohol
3.4
NAD+
Sulfolobus solfataricus
-
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
3.4
NAD+
Geobacillus stearothermophilus
-
mutant W49F/W167Y, 30°C, pH not specified in the publication
3.7
NAD+
Sulfolobus acidocaldarius
Q4J702
65°C, pH 8.0; pH 8.0, 65°C
4.67
NAD+
Coturnix coturnix
-
enzyme form ADH-2
5.1
NAD+
Geobacillus stearothermophilus
-
mutant Y25A, 30°C, pH not specified in the publication
6.18
NAD+
Coturnix coturnix
-
enzyme form ADH-3
6.3
NAD+
Geobacillus stearothermophilus
-
mutant Y25A/W49F/W87F, 30°C, pH not specified in the publication
7.75
NAD+
Mesocricetus auratus
-
oxidation of ethanol
8
NAD+
Geobacillus stearothermophilus
-
wild-type, 30°C, pH not specified in the publication
10.4
NAD+
Thermococcus kodakarensis
Q5JI09
cosubstrate 1-phenylethanol, pH 9.0, 70°C
18.5
NAD+
Sulfolobus solfataricus
-
carboxymethylated enzyme, at 55°C
19.3
NAD+
Sulfolobus acidocaldarius
-
65°C, pH 10.5
21
NAD+
Sulfolobus solfataricus
-
Km above 50 mM, mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
30.8
NAD+
Thermococcus kodakarensis
Q5JI09
cosubstrate 2,3-butanediol, pH 9.0, 70°C
33
NAD+
Geobacillus stearothermophilus
-
pH 7.7, 60°C
34.2
NAD+
Geobacillus stearothermophilus
-
wild-type, pH 8.0, 60°C
38.5
NAD+
Geobacillus thermodenitrificans
A4IP64, A4ISB9
cosubstrate 1-octanol, pH 8.0, 60°C
50
NAD+
Sulfolobus solfataricus
-
mutant enzyme N249Y, at 65°C, in the presence of benzyl alcohol
443.1
NAD+
Geobacillus thermodenitrificans
A4IP64, A4ISB9
cosubstrate ethanol, pH 8.0, 60°C
0.38
NADH

Sulfolobus solfataricus
-
mutant enzyme W95L, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.41
NADH
Aeropyrum pernix
-
pH 8.0, 60°C
2 - 8
NADH
Sulfolobus acidocaldarius
Q4J702
65°C, pH 5.5; pH 5.5, 65°C
2
NADH
Sulfolobus solfataricus
-
carboxymethylated enzyme, at 55°C
4.5
NADH
Sulfolobus solfataricus
-
native enzyme, at 55°C
4.5
NADH
Sulfolobus solfataricus
-
in 50 mM Tris-HCl (pH 7.5) containing 0.25 mM benzaldehyde, at 65°C
8.88
NADH
Coturnix coturnix
-
enzyme form ADH-3
10.3
NADH
Sulfolobus solfataricus
-
wild type enzyme, at 65°C, in the presence of benzaldehyde
10.3
NADH
Sulfolobus solfataricus
-
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
15.6
NADH
Thermococcus kodakarensis
Q5JI09
cosubstrate acetoin, pH 6.0, 70°C
17.3
NADH
Thermococcus kodakarensis
Q5JI09
cosubstrate acetoin, pH 6.0, 70°C
25.3
NADH
Sulfolobus solfataricus
-
mutant enzyme N249Y, at 65°C, in the presence of benzaldehyde
26.2
NADH
Sulfolobus acidocaldarius
-
65°C, pH 5.0
34.8
NADH
Sulfolobus solfataricus
-
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
43
NADH
Geobacillus stearothermophilus
-
pH 7.7, 60°C
52.4
NADH
Thermus thermophilus
-
65°C; pH 10.5, 65°C
326
NADH
Geobacillus stearothermophilus
-
wild-type, pH 8.0, 60°C
1646
NADH
Geobacillus thermodenitrificans
A4IP64, A4ISB9
cosubstrate acetaldehyde, pH 8.0, 60°C
10.5
NADP+

Thermococcus kodakarensis
Q5JI09
cosubstrate 1-phenylethanol, pH 9.0, 70°C
30
NADP+
Thermococcus kodakarensis
Q5JI09
cosubstrate 2,3-butanediol, pH 9.0, 70°C
43.9
NADP+
Geobacillus thermodenitrificans
A4IP64, A4ISB9
cosubstrate ethanol, pH 8.0, 60°C
194
NADP+
Yokenella sp.
W6CX26
pH 8.0, 55°C
283.1
NADP+
Geobacillus thermodenitrificans
A4IP64, A4ISB9
cosubstrate 1-octanol, pH 8.0, 60°C
433
NADPH

Yokenella sp.
W6CX26
pH 6.5, 65°C
4808
NADPH
Geobacillus thermodenitrificans
A4IP64, A4ISB9
cosubstrate octanal, pH 8.0, 60°C
3.33
octanal

Homo sapiens
-
-
17
octanal
Coturnix coturnix
-
enzyme form ADH-3
518.4
octanal
Geobacillus thermodenitrificans
A4IP64, A4ISB9
cosubstrate NADPH, pH 8.0, 60°C
0.038
Octanol

Homo sapiens
-
recombinant allozyme Ile308, pH 7.5, 25°C
0.04
Octanol
Homo sapiens
-
recombinant allozyme Val308, pH 7.5, 25°C
0.15
Octanol
Alligator mississippiensis
-
-
3.17
Octanol
Scyliorhinus canicula
P86884
pH and temperature not specified in the publication
4.33
Octanol
Homo sapiens
-
isoenzyme alpha,gamma1
7.33
Octanol
Homo sapiens
-
-
8.33
Octanol
Homo sapiens
-
-
2.92
pentan-1-ol

Coturnix coturnix
-
enzyme form ADH-3
2.92
pentan-3-ol
Sulfolobus solfataricus
-
-
0.245
Pentanol

Homo sapiens
-
-
0.283
Pentanol
Saimiri sciureus
-
pyrazole-sensitive enzyme, pH 7.5
0.333
Pentanol
Saimiri sciureus
-
pyrazole-sensitive enzyme, pH 10.0
0.667
Pentanol
Homo sapiens
-
pH 7.5, anodic enzyme form
8
Pentanol
Homo sapiens
-
-
16
Pentanol
Homo sapiens
-
-
18.4
Pentanol
Equus caballus
-
mutant enzyme W54L
56.2
Pentanol
Equus caballus
-
wild-type enzyme Adh 1
202
phenylacetaldehyde

Rhodococcus ruber
-
pH 6.0, 37°C, recombinant enzyme expressed from Saccharomyces cerevisiae
264
phenylacetaldehyde
Rhodococcus ruber
-
pH 6.0, 37°C, recombinant enzyme expressed from Hansenula polymorpha
303
phenylacetaldehyde
Rhodococcus ruber
-
pH 6.0, 37°C, recombinant enzyme expressed from Arxula adeninivorans
5.8
propan-2-ol

Equus caballus
-
mutant enzyme W54L
6.92
propan-2-ol
Sulfolobus solfataricus
-
-
53.1
propan-2-ol
Equus caballus
-
wild-type enzyme Adh 1
17.2
Propanol

Homo sapiens
-
-
28.7
Propanol
Equus caballus
-
mutant enzyme W54L
0.087
retinol

Homo sapiens
-
recombinant allozyme Ile308, pH 7.5, 25°C
0.11
retinol
Homo sapiens
-
recombinant allozyme Val308, pH 7.5, 25°C
0.21
trans-cinnamaldehyde

Sulfolobus solfataricus
-
mutant enzyme W95L,, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
2.1
trans-cinnamaldehyde
Sulfolobus solfataricus
-
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
18.9
trans-cinnamaldehyde
Sulfolobus solfataricus
-
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
418
Valeraldehyde

Rhodococcus ruber
-
pH 6.0, 37°C, recombinant enzyme expressed from Saccharomyces cerevisiae
432
Valeraldehyde
Rhodococcus ruber
-
pH 6.0, 37°C, recombinant enzyme expressed from Arxula adeninivorans
528
Valeraldehyde
Rhodococcus ruber
-
pH 6.0, 37°C, recombinant enzyme expressed from Hansenula polymorpha
0.467
Vanillyl alcohol

Homo sapiens
-
-
8.67
Vanillyl alcohol
Homo sapiens
-
-
additional information
additional information

Homo sapiens
-
turnover-numbers for the class I isoenzymes with the substrates ethanol, methanol, ethylene glycol, benzyl alcohol, octanol, cyclohexanol and 16-hydroxyhexadecanoic acid
-
additional information
additional information
Equus caballus
-
-
-
additional information
additional information
Equus caballus
-
Km-values of active-site Co(II)substituted enzyme
-
additional information
additional information
Drosophila melanogaster
-
kinetics of ethanol oxidation
-
additional information
additional information
Drosophila simulans
-
kinetics of ethanol oxidation
-
additional information
additional information
Homo sapiens
-
kcat for isozymes ADH1B1, ADH1B2, and ADH4 for all retinoid substrates in forward and reverse reaction
-
additional information
additional information
Mus musculus
-
kcat for isozymes ADH1, and ADH4 for all retinoid substrates in forward and reverse reaction
-
additional information
additional information
Mus musculus
-
effects of tert-butanol, butyramide, valeramide and capronamide on turnover-number of ethanol
-
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